DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Stim and NST1

DIOPT Version :9

Sequence 1:	NP_523357.2	Gene:	Stim / 32556	FlyBaseID:	FBgn0045073	Length:	570	Species:	Drosophila melanogaster
Sequence 2:	NP_014308.1	Gene:	NST1 / 855633	SGDID:	S000005035	Length:	1240	Species:	Saccharomyces cerevisiae

Alignment Length:	651	Identity:	121/651 - (18%)
Similarity:	227/651 - (34%)	Gaps:	190/651 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly    20 SISTLDHGPHTVSVDSNRHNTQHQYKQ-NPNVASQRHSSHESGQSLHN--SQSEHVTHIAASHAG 81
            |....|....|..:.|.:..|:.:|.. ..|:|.:..:::::......  ||.|.
Yeast   346 STDLTDPKEFTTMLHSGKPLTEDEYADLQRNIAERMTNAYDTASKKFKDVSQLEK---------- 400

  Fly    82 SGGEHSTHLAQNLHRSSYNLLSEAMSQAVSNEF--------------SSMGSGSADGACAAD--- 129
               |..|.......:.|:.   |.:.|:..|:|              |...|.|.|.:...|
Yeast   401 ---ELFTRFMSGRDKKSFR---ELIIQSFKNKFDGELGPSVLAATLSSCFSSQSKDTSLDTDSIY 459

  Fly   130 ------DFDCYSGSVQDR------FGMEAI-------------ASLHRQLDDD--------DNGN 161
                  |:|.||...:|.      .|:||:             .:||...|.|        .:.:
Yeast   460 EDEDEEDYDDYSEYAEDSEEVSEYEGIEAVEKPEHDEKSNGIRETLHLSYDHDHKRQNHPHHHYH 524

  Fly   162 IDLSESDDFLREELKYDSGYE---KRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTD-WL 222
            ...:.|:|.|.|| :|.|..|   ...|.||.:||:....::  |.....|......|.|.| .|
Yeast   525 STSTHSEDELSEE-EYISDIELPHDPHKHFHRDDDILDGDED--EPEEEDENEGDDEEDTYDSGL 586

  Fly   223 AQSVQLPQYVDLFKLHKVTGAALPRLAVNNL------------QYVGNVLGIKDPIHKQKISLKA 275
            .::.:|.:           |..|.::|:..|            |...|.|.:...:.::|...:
Yeast   587 DETDRLEE-----------GRKLIQIAITKLLQSRIMASYHEKQADNNRLKLLQELEEEKRKKR- 639

  Fly   276 MDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKNAKRHLRRMAQDMEGLQRAEQSLQE 340
                    .:|...:.|                   ::.:..|:.|:::|::.|..:|.|:. :.
Yeast   640 --------EKEEKKQKK-------------------REKEKEKKRLQQLAKEEEKRKREEEK-ER 676

  Fly   341 MQKELERARMEQENVATEKLDLERRLKEAPTLSSSNSDLEVQQLKKEIEMLRNELSRAEFELVDN 405
            ::||||...|.:.....:|::..:|.|:    ......||.||.::|::    |..|.:.|
Yeast   677 LKKELEEREMRRREAQRKKVEEAKRKKD----EERKRRLEEQQRREEMQ----EKQRKQKE---- 729

  Fly   406 CWSPPPQLQSWLQYTYELESK-NHQKKRTSAEKQLQSAREACEKLRKKRSSLVGAFVSTHGK-SI 468
                            ||:.| ..:|||...:|:|:..:...||..::|..|:........| :.
Yeast   730 ----------------ELKRKREEEKKRIREQKRLEQEKLQKEKEEEERQRLIAEDALRKQKLNE 778

  Fly   469 DDVDRSIVEAR----NALGD-VTNELQERLHRWKQIETC---------------------LGLNI 507
            :....:|:.|:    |.:|: |:::....:..:::..:|                     .|.|:
Yeast   779 EQTSANILSAKPFTENGVGNPVSSQSHPNMTNYQEDNSCSINDEILKMVNSVAASKPVSPTGFNV 843

  Fly   508 ------VNNNGLPYLENVLYGRNGGLQSSMGMSSTKGSRARITNSTEDLDDESIQGKLNFENFSL 566
                  ..||.:|.:|.....:.|...:..|.::...:....|.|:...::..:......||.||
Yeast   844 HDLLLPSTNNQMPAMEQSHLPQPGNQNNHFGTTTIPNALDLATKSSLQTENNYLMNSQTLENTSL 908

  Fly   567 L 567
            |
Yeast   909 L 909

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Stim	NP_523357.2	SAM_STIM-1,2-like	209..282	CDD:188903	14/85 (16%)
		SAM	210..271	CDD:197735	12/73 (16%)
		GBP_C	<312..392	CDD:303769	19/79 (24%)
		coiled coil	360..372	CDD:293879	2/11 (18%)
		coiled coil	381..392	CDD:293879	3/10 (30%)
		SOAR	410..507	CDD:293141	20/124 (16%)
NST1	NP_014308.1	NST1	<103..159	CDD:372833
NST1	NP_014308.1	ARGLU	<694..727	CDD:405931	10/40 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0.878984	Normalized mean entropy	S4367
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.950