Protein Alignment Graf and RGA2

DIOPT Version :9

• Sequence 1: NP_001285296.1
• Gene: Graf / 32522
• FlyBaseID: FBgn0030685
• Length: 1025
• Species: Drosophila melanogaster
• Sequence 2: NP_010667.1
• Gene: RGA2 / 851985
• SGDID: S000002787
• Length: 1009
• Species: Saccharomyces cerevisiae
• Alignment Length: 613
• Identity: 131/613 - (21%)
• Similarity: 222/613 - (36%)
• Gaps: 171/613 - (27%)

  Fly   102 IGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKE-----NKKKFDKKTEKFCQSQERFLNMST 161
            :.|::.:..:||.....|...|:::.......||..     .|.|..|......||.....|...
Yeast   442 VHNLKSKTSEMLDPKHPHHSTSIQESDTHSGWGVSSTHTNIRKSKAKKNPVSRGQSDSTIYNTLP 506

  Fly   162 KKPENTIQEADASLGMHEREYIQESLSYVLRIQE-----VQERIKFEFVEIL----LAFI----- 212
            :....|:.|.:     |::  .|.||..:.:.|.     ...||...|....    :|..
Yeast   507 QHGNFTVPEFN-----HKK--AQSSLGSISKKQNSNDTATNRRINGSFTSSSSGHHIAMFRTPPL 564

  Fly   213 -SGWLVFYHTAHEQAEDHRD-----------YLQDLRHKVQKTRENFEEAREK---VTELKTKYM 262
             ||.|....:...::..||.           .|:|...||..|.|: ..:.||   .|||..:.:
Yeast   565 ESGPLFKRPSLSSESAHHRSSSLQTSRSTNALLEDDSTKVDATDES-ATSLEKDFYFTELTLRKL 628

  Fly   263 EKRTKPEEIFTKRGYLFLMEKSSILKISLLEPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFT- 326
            :...:..| .||:..|..:|...:.|..||....      ..|.:|.|:..:..:..:...|..
Yeast   629 KLDVRELE-GTKKKLLQDVENLRLAKERLLNDVD------NLTREKDKQSASSRESLEQKENIAT 686

  Fly   327 -----RPEARDDEKLTLFSCQRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDH---------R 377
                 .|.:..|.|.::.:...: ..|.|.|.                  |.|||         |
Yeast   687 SITVKSPSSNSDRKGSISNASPK-PRFWKIFS------------------SAKDHQVGDLESQQR 732

  Fly   378 YWISAMDGT---------EPTYL-------APGKIKVS----------EAYHLDEAGF------- 409
            ...|:..||         .|..:       :|||:.:|          :..||..:..
Yeast   733 SPNSSSGGTTNIAQKEISSPKLIRVHDELPSPGKVPLSPSPKRLDYTPDGSHLYGSSLQARCAYE 797

  Fly   410 -----MFIRRCIQVLEIR--GLEDEGIYRKSGVGTKISKL--------------LALGLNQKESD 453
                 :.||.||..:|..  ||..||:|||||..|.:.::              |:..||...:.
Yeast   798 KSTVPIIIRCCIDRIEKDDIGLNMEGLYRKSGSQTLVEEIENEFAQNNSLHSDTLSPKLNALLNQ 862

  Fly   454 DVFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRV---NE------ 509
            |:            :.:||.||.|||.|.:|::::..:...|:..:...|.:|:   |:
Yeast   863 DI------------HAVASVLKRYLRKLPDPVLSFSIYDALIDLVRNNQLIERLPLNNDKFLDSP 915

  Fly   510 ----VHKLVYK--------LPQPNFQMLDMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPRE 562
                ::::|.|        ||..:.::|.::..|:..|.|..|:|.|::.||.:||.|:|:.. .
Yeast   916 QKVTIYEMVLKSLLEIFKILPVEHQEVLKVLAAHIGKVRRCSERNLMNLHNLSLVFAPSLIHD-F 979

  Fly   563 ESVAAILDIKFNNIVINILIDNYERIFK 590
            :....|:|:|..|.::..::.||..|||
Yeast   980 DGEKDIVDMKERNYIVEFILGNYRDIFK 1007

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Graf	NP_001285296.1	BAR_RhoGAP_OPHN1-like	27..233	CDD:153286	29/161 (18%)
		BAR-PH_GRAF_family	273..390	CDD:269953	26/140 (19%)
		PH	279..383	CDD:278594	20/118 (17%)
		RhoGAP	383..584	CDD:295372	62/275 (23%)
		SH3_GRAF-like	967..1020	CDD:212815
RGA2	NP_010667.1	LIM1_Rga	13..67	CDD:188780
		LIM2_Rga	70..123	CDD:188781
		RhoGAP	815..1002	CDD:214618	48/199 (24%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm9247
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1094
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	2.030