DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Graf and AGD2

DIOPT Version :9

Sequence 1:	NP_001285296.1	Gene:	Graf / 32522	FlyBaseID:	FBgn0030685	Length:	1025	Species:	Drosophila melanogaster
Sequence 2:	NP_176283.1	Gene:	AGD2 / 842378	AraportID:	AT1G60860	Length:	776	Species:	Arabidopsis thaliana

Alignment Length:	772	Identity:	134/772 - (17%)
Similarity:	258/772 - (33%)	Gaps:	244/772 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    22 DSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLNDFNFECIGTAQTD 86
            |||.|::.:...|...|....:.:::.|.||..|.|....||.:...|..|.:|     |....|
plant     9 DSPMFQKQVFSLEGTSDELKDRCQKLYKGVKKFMGALGEASTGVSAFADSLEEF-----GAGHDD 68

  Fly    87 DENVICES--LKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENKKKFDKKTEKF 149
            ..:|....  :.:|...:..:...:|.:.:..:..::|.|.:|....:...||::::|||....:
plant    69 PVSVSIGGPVISKFINTLRELSSYKEFLRSQVEHVLLERLTNFMTVDLQEAKESRRRFDKAVHSY 133

  Fly   150 CQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEFVEILLAFISG 214
            .|::|:|:::......:.:.|.:..|...:..:.:...:.|..:..::.:.|:||:|.:.|.:..
plant   134 DQAREKFVSLKKNTRGDIVAELEEDLENSKSAFEKSRFNLVNSLMTIEAKKKYEFLESISAIMDS 198

  Fly   215 WLVFYHTAHEQAEDHRDYLQDLRHKVQKTRE-----------NFEEAREKVTELKTKYMEKRTKP 268
            ...::...::.......|:..:....|:::|           ..:|.|.: :||.::....:..|
plant   199 HFKYFKLGYDLLSQLEPYIHQVLTYAQQSKEQSKIEQDRFAQRIQEFRTQ-SELDSQQASAKADP 262

  Fly   269 EEI-------------------------FTKRGYLFLMEKSSILKISLLEPFKATWTKYYCTFKK 308
            .::                         .||:||  |:::|:.|        :|.|
plant   263 SDVGGNHVYRAIPRKNVEANSVSTADKEVTKQGY--LLKRSASL--------RADW--------- 308

  Fly   309 QKREFTMLQFNQMNHN-----------------------------FTRPEARDDEKLTLFS--C- 341
             ||.|.:|.    ||.                             |.|...|.:...:..|  |
plant   309 -KRRFFVLD----NHGSLYYYRNTGNKSAKSQHYYSGLGEHSSGVFGRFRTRHNRSASQGSLDCN 368

  Fly   342 ---------QRRASEFEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGKIK 397
                     :..|.:.:.|.||.:...:|   .||.||.:..|...|::.:.......|....::
plant   369 MIDLRTSLIKLDAEDTDLRLCFRIISPQK---TYTLQAENGADRMDWVNKITAAITIRLNSHFLQ 430

  Fly   398 VSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKE--------SDD 454
            .|.|.:||:..                      ..||..|:     .|.|||||        .||
plant   431 QSPARYLDKKN----------------------TSSGPATE-----NLTLNQKEDYNQRLNVGDD 468

  Fly   455 VFVDDKYRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQ 519
            |..  ..|::..:||.|..                                          ..|.
plant   469 VLT--ILREIPGNNTCAEC------------------------------------------NAPD 489

  Fly   520 PNFQMLDMVICHLTDVSRKYEKNKMSVFNLGV-------------VFGPTLLRPREESVAAILDI 571
            |::..|::.:....:.|..:.       ||||             |:.||           |||:
plant   490 PDWASLNLGVLMCIECSGVHR-------NLGVHISKVRSLTLDVKVWEPT-----------ILDL 536

  Fly   572 KFNNIVINILIDNYERIFKNKPSADIKLPDATKAPSIMYPSRSSPPT--------RMPRASQIGK 628
             |.|:........:|.:..:...      |:.|..:....|.|.|.:        :......:.|
plant   537 -FRNLGNGYCNSVWEELLHHLDD------DSEKGSTDTLASVSKPSSEDWFTLKEKYINGKYLEK 594

  Fly   629 AASTGAMGSSSNTAGNPMF-----LNQQKIYRVVSKTNCTEPTMSSSLQNIPNGDNY 680
            |........:::||.:.::     .|.:.|||::.|.:..  .:::...:|.:.|.|
plant   595 ALVVKDEREANSTASSRIWEAVQSRNIRDIYRLIVKADAN--IINTKFDDITDLDVY 649

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Graf	NP_001285296.1	BAR_RhoGAP_OPHN1-like	27..233	CDD:153286	36/207 (17%)
		BAR-PH_GRAF_family	273..390	CDD:269953	31/157 (20%)
		PH	279..383	CDD:278594	27/144 (19%)
		RhoGAP	383..584	CDD:295372	37/221 (17%)
		SH3_GRAF-like	967..1020	CDD:212815
AGD2	NP_176283.1	BAR_SFC_plant	15..216	CDD:153290	36/205 (18%)
		PH	291..420	CDD:278594	31/155 (20%)
		PH_ACAP	293..425	CDD:270070	30/158 (19%)
		ArfGap	470..604	CDD:214518	28/202 (14%)
		ANK repeat	676..714	CDD:293786
		ANK	<684..763	CDD:238125
		Ank_4	686..737	CDD:290365
		ANK repeat	716..747	CDD:293786
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910