DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Graf and Ophn1

DIOPT Version :9

Sequence 1:	NP_001285296.1	Gene:	Graf / 32522	FlyBaseID:	FBgn0030685	Length:	1025	Species:	Drosophila melanogaster
Sequence 2:	NP_001101318.1	Gene:	Ophn1 / 312108	RGDID:	1563435	Length:	802	Species:	Rattus norvegicus

Alignment Length:	900	Identity:	305/900 - (33%)
Similarity:	441/900 - (49%)	Gaps:	152/900 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 GLEPLEFEECIVDSPDFRENLNRHEKELDHTSHQIKRIIKEVKDLMSAAKILSTRMKQLAILLND 74
            |..||||.:|.:|||||||.|..:|:||:.|:..||.:||:...|:||.:..|:.:::.:..|..
  Rat     2 GHPPLEFSDCYLDSPDFRERLKCYEQELERTNKFIKDVIKDGSALISAMRSYSSAVQKFSQTLQS 66

  Fly    75 FNFECIGTAQTDDENVICESLKRFGAIIGNIEDEREKMLTLADKHIIESLEDFRKKQIGGVKENK 139
            |.|:.||...||||..|.||.|.|..::..:|:||..|:..|...:|:.||.|||:|||..||.|
  Rat    67 FQFDFIGDTLTDDEINIAESFKEFAELLNEVENERMMMVQNASDLLIKPLETFRKEQIGFTKERK 131

  Fly   140 KKFDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHEREYIQESLSYVLRIQEVQERIKFEF 204
            |||:|..|:|....:|.|::|:||.|:.:.|||..:......:.:.||.||.:||||||..||..
  Rat   132 KKFEKDGERFYSLLDRHLHLSSKKKESQLLEADLQVDKERHNFFESSLDYVYQIQEVQESKKFNI 196

  Fly   205 VEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRENFEEAREKVTELKTKYME--KRTK 267
            ||.:|||:....:......|..:|...|.|.|:..:|.||.:|...||::.|||.:..|  :..|
  Rat   197 VEPVLAFLHSLFISNSLTVELTQDFLPYKQQLQLSLQNTRNHFSSTREEMEELKKRMKEAPQTCK 261

  Fly   268 PEEIFTKRGYLFLMEKSSILKISLLEPFKATWTKYYCTFKKQKREFTMLQFNQMNHNFTRPEARD 332
            .....|..|||:..||.: |.||        |.||||.::|:.|..||....|      :|.|:.
  Rat   262 LPGQPTIEGYLYTQEKWA-LGIS--------WVKYYCRYEKETRTLTMTPTEQ------KPGAKQ 311

  Fly   333 DE-KLTLFSCQRRASE-FEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAMDGTEPTYLAPGK 395
            .. .|||..|.||.:| .:||||||:...|:.|.: |.||.||.:.|.|:.||||.||.|.:|
  Rat   312 GPVDLTLKYCVRRKTESIDKRFCFDIEANERTGTI-TLQAPSEANRRLWMEAMDGKEPIYHSP-- 373

  Fly   396 IKVSEAYHLDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDK 460
            |...|...|:|.||.|:|:||..:|.:|::.||:||..|...::.|||....:.|...||   |.
  Rat   374 ITKQEEMELNEVGFKFVRKCINFIETKGIKTEGLYRTVGSNIQVQKLLNAFFDPKCPGDV---DF 435

  Fly   461 YRDLMESNTIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQML 525
            |....:..||.|:||.|||||:||:|||:.|.:.:.|||.:.|:.|:..:|.||||||:.|.:||
  Rat   436 YNSDWDIKTITSSLKFYLRNLSEPVMTYKLHKELVSAAKSDNLDYRLGAIHSLVYKLPEKNREML 500

  Fly   526 DMVICHLTDVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKFNNIVINILIDNYERIFK 590
            :::|.||.:|....::|.|:..|:||:|||||:|.:|::|||:::|||.|||:.|||:::.:|:.
  Rat   501 ELLIKHLVNVCEHSKENLMTPSNMGVIFGPTLMRAQEDTVAAMMNIKFQNIVVEILIEHFGKIYL 565

  Fly   591 NKPSADIKLPDATKAPSIMYPSRSSPPTRMPRASQIGKAASTGAMGSSSNTAGNPMFLNQQKIYR 655
            ..|. |.::|..  .|..:...|..|.|...|                        .|.::.::.
  Rat   566 GPPE-DSQVPPV--PPPRVTARRHKPITISKR------------------------LLREKAVFY 603

  Fly   656 VVSKTNCTE----PTMSSSLQNIPNGDNYALGSNAMNSSGGAQCISLSPPMHMLNGILSPTIGSI 716
            ..|..:..:    ||        ||      |:.|.|.....|...|..||.. :|.:.|
  Rat   604 TPSLDDVEDEIHHPT--------PN------GTIASNLDPPKQLQHLKLPMQK-SGEMDP----- 648

  Fly   717 NNLHTISKNETSTRRYVPDTEIAP----DYG--IIGANNGGVTLQSHHAVPVSSTSNFRHPEYLM 775
                    ...|..|.|.|.:..|    |.|  :....:||.     .|:|.:|.
  Rat   649 --------GRKSPSRPVSDCQTEPCLEADMGKLVYRLQDGGT-----KAIPKASN---------- 690

  Fly   776 TTANPVTQSGSSSHIYTNTSS-----------AGANSSNRISLSNVSPP---NTAMRKERFLGSA 826
               .||..||     :|.|||           ......:....|.|.||   .|.:|
  Rat   691 ---GPVPGSG-----HTKTSSFHIKRPAPRPIVHHKEGDTDCFSKVRPPGEKQTIIR-------- 739

  Fly   827 SGPQQHPPVQRGLHSYGQTKHYSPLMPTSTSSSNDSVCDSLSSN-NGLGSSIVAN 880
              |...||              .|...:|||...:|..:::||| ..:.||:||:
  Rat   740 --PPVRPP--------------DPPCRSSTSQKPESKPETVSSNAEEIPSSVVAS 778

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Graf	NP_001285296.1	BAR_RhoGAP_OPHN1-like	27..233	CDD:153286	80/205 (39%)
		BAR-PH_GRAF_family	273..390	CDD:269953	49/118 (42%)
		PH	279..383	CDD:278594	39/105 (37%)
		RhoGAP	383..584	CDD:295372	91/200 (46%)
		SH3_GRAF-like	967..1020	CDD:212815
Ophn1	NP_001101318.1	BAR_OPHN1	19..225	CDD:153317	80/205 (39%)
		PH	266..363	CDD:278594	43/112 (38%)
		BAR-PH_GRAF_family	267..370	CDD:269953	49/118 (42%)
		RhoGAP_Graf	363..559	CDD:239839	91/200 (46%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	641..663		6/35 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	682..802		32/143 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166339957
Domainoid	1	1.000	237	1.000	Domainoid score	I2240
eggNOG	1	0.900	-	-		E1_KOG1451
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	516	1.000	Inparanoid score	I1242
OMA	1	1.010	-	-		QHG45894
OrthoDB	1	1.010	-	-		D306399at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm46182
orthoMCL	1	0.900	-	-		OOG6_101903
Panther	1	1.100	-	-	O	PTHR12552
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X614
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	14	13.770