Protein Alignment Graf and rga3

DIOPT Version :9

• Sequence 1: NP_001285296.1
• Gene: Graf / 32522
• FlyBaseID: FBgn0030685
• Length: 1025
• Species: Drosophila melanogaster
• Sequence 2: NP_594871.1
• Gene: rga3 / 2542306
• PomBaseID: SPAC29A4.11
• Length: 969
• Species: Schizosaccharomyces pombe
• Alignment Length: 646
• Identity: 132/646 - (20%)
• Similarity: 248/646 - (38%)
• Gaps: 140/646 - (21%)

  Fly     2 GGGKNVRRGLEPLE-FEECIVDSPDFRENLNRHEKELDHTS--HQIKRIIKEVKDLMSAAKILST 63
            ||.|:.|......| .::.:|  |....|...|....||:|  .|.:| ..:|::|....:.|:.
pombe   407 GGHKSFRHSHSLSETSQQTLV--PSLGSNGEYHLPTNDHSSTPAQSER-DSDVEELREQLENLTA 468

  Fly    64 RMKQLAILLNDFNFECIGTAQTDDENV-------ICESLKRFGAIIGN--IEDEREKMLTLADKH 119
            ..|:|:..|:...|:.....:|:|::.       |||....|..:..:  ::|.:.:.|..|...
pombe   469 LTKKLSERLSSSTFDNSKFIRTEDKDTVRSAKLEICEKFFSFADVTDDPTLKDPKHQDLVAAANA 533

  Fly   120 IIESLEDFRKKQIGGVKENKKK----FDKKTEKFCQSQERFLNMSTKKPENTIQEADASLGMHER 180
            .:..|.:....:|..:.|.:.:    ::...:...:|.|..::::||    .::.||.:..:
pombe   534 YMAMLRESYGTEINNLLERRNELLDDYNNVQKILNESLEASVHLNTK----NLELADLNNNL--- 591

  Fly   181 EYIQESLSYVLRIQEVQERIKFEFVEILLAFISGWLVFYHTAHEQAEDHRDYLQDLRHKVQKTRE 245
                        ::::|.|:..|                    .|:        :|.|.:..:.:
pombe   592 ------------VKQIQHRVPPE--------------------NQS--------NLEHTITTSSK 616

  Fly   246 NFEEAREKVTELKTKYMEKRTKPE------------EIFTKRGYLFLMEKSSILKISLLEPFKAT 298
            |...:...:|.:.:...:...:|.            :|..|:|.:.|..:....|:    |||:.
pombe   617 NTTSSINPLTAVSSNSGQSSGRPGPLSPNLNVTTRIDIKGKKGSMHLQPRDVNRKV----PFKSM 677

  Fly   299 WTKYYCTFKKQKREFTMLQFNQMNHNF-----TRP-------EARDDEKLTLF----SCQRRASE 347
            .||..........|    ...|.:|.|     .:|       |:....:|..|    ||..|
pombe   678 HTKSKSADPVVGNE----DRTQCDHVFHVNAIFKPSRCYICSESVWGSELRCFHCSISCHSR--- 735

  Fly   348 FEKRFCFDLTFKEKPGVVYTFQALSEKDHRYWISAM------DGTEPTYLA---PGKIKVSEAYH 403
                 |....|.|........:.:||...  |:..|      .|..||...   ..::|: |...
pombe   736 -----CLKRLFAESEHEKTMSETVSENSK--WMPEMPTRMPPPGPSPTMFGRSLENQLKI-EGSV 792

  Fly   404 LDEAGFMFIRRCIQVLEIRGLEDEGIYRKSGVGTKISKLLALGLNQKESDDVFVDDKYRDLMESN 468
            |.:.    |..|:..::..|||.|||||.||..:::..|    :::.|:..:.::....||.
pombe   793 LPQV----IAMCVSCVDAHGLEVEGIYRISGSASQVRVL----VDEFENGSIRMEHLTSDLF--- 846

  Fly   469 TIASALKMYLRNLNEPLMTYQYHSDFIEAAKQETLNQRVNEVHKLVYKLPQPNFQMLDMVICHLT 533
            ...|.||.||..|.||::....:.:.:||.|.|...:::..|.:::..|...:..:...:|.||.
pombe   847 ACTSVLKTYLHRLPEPVIPGTQYEELLEAEKIEKEEEKIERVVEVMKTLHPAHLSVFRFLIAHLG 911

  Fly   534 DVSRKYEKNKMSVFNLGVVFGPTLLRPREESVAAILDIKF---NNIVINILIDNYERIFKN 591
            .|.:..|||.|:..|:..||.|||:|.:...    .|::.   .:..:..::||.::|..|
pombe   912 RVCKHAEKNLMNSKNVSTVFAPTLMRDKVNR----FDLQHATKKSTALQFMLDNVDKILHN 968

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Graf	NP_001285296.1	BAR_RhoGAP_OPHN1-like	27..233	CDD:153286	36/220 (16%)
		BAR-PH_GRAF_family	273..390	CDD:269953	29/138 (21%)
		PH	279..383	CDD:278594	24/119 (20%)
		RhoGAP	383..584	CDD:295372	53/212 (25%)
		SH3_GRAF-like	967..1020	CDD:212815
rga3	NP_594871.1	LIM	18..74	CDD:214528
		LIM	78..128	CDD:259829
		C1	698..743	CDD:237996	11/52 (21%)
		RhoGAP	791..962	CDD:214618	48/185 (26%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1094
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	1.030