DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and Hecw1

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	XP_006516872.1	Gene:	Hecw1 / 94253	MGIID:	2444115	Length:	1605	Species:	Mus musculus

Alignment Length:	1718	Identity:	390/1718 - (22%)
Similarity:	626/1718 - (36%)	Gaps:	476/1718 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly  3627 VDASVGAQTPATT--------------EEAASTAGASGTTA-TITDMSPEVRAALGDLEVPEGV- 3675
            |..::.|.||:.|              ||.|.:.|:....: :::|..     |:|   :.:|:
Mouse   167 VSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQ-----AMG---LKKGMF 223

  Fly  3676 ---DP-----------SFLAALP---SEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSL-VEVNP 3722
               ||           |...|||   .|.|..:|..  .:..|.|....:.:.:..|.| :||..
Mouse   224 FNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGN--TVNPIWQAEHFSFVSLPTDVLEIEVKD 286

  Fly  3723 EFLAALP--------LNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDM 3779
            :|..:.|        |::..:.|:::.....|..:.|.....|.|..:........:..:|..|.
Mouse   287 KFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADD 351

  Fly  3780 EESQIASLPPELAAEAQ--FLRRDWESRNGAR--------MDAHPPS----NALSRFQTTLQSLD 3830
            ||..: |..||.:||.|  .:.....:.||..        .||.|.|    |.::      .|.:
Mouse   352 EEISL-SAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVN------SSEN 409

  Fly  3831 DTRWHSSIWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMED--ENILLDPDSLATL 3893
            ..:.|:....:|:|..:.:...:.:...:.|.....|........|..|:  |.:.||.||.::|
Mouse   410 QNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSL 474

  Fly  3894 L------LFLFVEDQKV--NSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNEDNANFGAGQAG 3950
            |      |...||::.|  |..|...:.:.....|...|      :|.:::          |:.|
Mouse   475 LPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEED------VSTLEQ----------GEPG 523

  Fly  3951 GKPEWLKLRVDAAFGYKSNIFLINRLYEGSARSISINPQAAQMIVRNCLDLLFVLAKHYPSSFVP 4015
                 |:|||..               ...:|..|:.....:.::.:..........||
Mouse   524 -----LELRVSV---------------RKKSRPCSLPVSELETVIASACGDAETPRTHY------ 562

  Fly  4016 FNRDVKARRILSAVFGMPSPTAGSGGGAGSG---------SAASGGLTGYDDV-----------P 4060
                   .||.:.:..|||...||......|         |:...||:..|.:           .
Mouse   563 -------IRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGES 620

  Fly  4061 PGGTLDFRSRFC---RYQVVNRLRSIFDD---HP---APKPSVPLDEAPSTSKAAAAKAAATAKV 4116
            .|.||......|   .:..:::......|   ||   :...|.|...|..:.:...|...:.:..
Mouse   621 DGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCY 685

  Fly  4117 STA--GSQNKHSVPYQTNAKNFWDVVLNIDQQTQVRLANVAQFPLTSQPAWEWQPNTADFLSFSE 4179
            ||:  .|....|..|.::..|           ...|.|:..:|            ::.|....||
Mouse   686 STSCYSSSCYSSSCYSSSCYN-----------GNNRFASHTRF------------SSVDSAKISE 727

  Fly  4180 SPFGQLLEMLPYKVICRSPHLTDMLVKLLASLSTELPKEE-----ELLPLSDQMPPLVP-----I 4234
            |                            ...|::..:||     |.:|.|.|.|.|.|     .
Mouse   728 S----------------------------TVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGA 764

  Fly  4235 GPVQRAGAAAAAAHPPPPQPPAENTATATEIGDGLAANIEEQASPASGNPASGDSNAGAKRTQLP 4299
            ||.|...||           |..|.|.:||   ||.            :|.:|.||  .:..:.|
Mouse   765 GPWQDELAA-----------PGGNAARSTE---GLE------------SPMAGPSN--RREGECP 801

  Fly  4300 TLSVALPVT--PS-KPRRKIYSKNFSQLQLVIEVLTHQCGTTEGLDNVAKLIVNLTQCSQASSAI 4361
            .|..:.|::  || :|....|......|....|......|....:|:     :|.|...|..|
Mouse   802 ILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDH-----INRTTTWQRPS-- 859

  Fly  4362 FIQYLTAAILGL--AEEVRQAIQMLLNEIRMYNIHQ-------------------------GGAG 4399
                :.....|:  :..|.|..|  ||. |..||.:                         ||.|
Mouse   860 ----MAPTPDGMIRSGSVHQMEQ--LNR-RYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGG 917

  Fly  4400 TTSQAASTSGAAAATASYVPTVSGASLLPNLAVHEGIMQDRFTAEHVIISAPKNA-KPTCELQLP 4463
            :.|:|.|:..:....                  .||.:            :|.|: |.|..||.|
Mouse   918 SDSEAESSQSSLDLR------------------REGSL------------SPVNSQKVTLLLQSP 952

  Fly  4464 SMK---------KLMSNSSAQPYFLRT-------LKIFMQVRDMYEAQNGLSTGGGVACGAGDND 4512
            ::|         .|.:|.||...|..:       ||:....|:....|               ::
Mouse   953 AVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQ---------------HN 1002

  Fly  4513 DDII-----------------DIEGDGGASGGSSSDMAPGSAST--ISPPPPMETNQIAQD---- 4554
            .|::                 :|:.|   ..|.|..:...|.:|  |.|..|::..::...
Mouse  1003 RDLVNFINMFADTRLELPRGWEIKTD---HQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHR 1064

  Fly  4555 -----------GDDDEEDDDEARSFPGVGNHAVASASKSTKPTLSQILCLDVLWNTLSECLVALE 4608
                       |:..|...:...|......|::.:|.:|.    .|...|.:.:|
Mouse  1065 QHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQ----HQHESLPLAYN---------- 1115

  Fly  4609 ESKDEFAVLVLQPTVEAFFLIHASH----RGASKKSGRSFMPNAAGFGL-RAAAGAGAIGGLGAA 4668
               |:....:.||.:  |.::....    |..:.:....::......|| :.:..|..:..|...
Mouse  1116 ---DKIVAFLRQPNI--FEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLF 1175

  Fly  4669 APSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFL 4733
            ......|.|    :.||..|   .:...||....|       :|..|..:....|:
Mouse  1176 EEEIMSYVP----LQSAFHP---GYSFSPRCSPCS-------SPQNSPGLQRASAR--------- 1217

  Fly  4734 QFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSV 4798
                                 :..|:.        .||:.|.:.|..:||....|....:..:.:
Mouse  1218 ---------------------APSPYR--------RDFEAKLRNFYRKLEAKGFGQGPGKIKLII 1253

  Fly  4799 RRVTVFEDSF-RVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSP 4862
            ||..:.|.:| :|:.....|..:|:.||.|..|||.|..|..||::.::|:|:|||.|.||..|.
Mouse  1254 RRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSA 1318

  Fly  4863 GDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQD 4927
            .|..|..|:|.|....|:|.:|:|.||::..|:....||:.:|||.|||.:|......:|:|..|
Mouse  1319 NDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLD 1383

  Fly  4928 YEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKM 4992
            .||::.|.::..|:|:.: .:|||:...:.||....|:||..|.:|.|||:||.||::.:.:.::
Mouse  1384 EEFHQSLQWMKDNNITDI-LDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRV 1447

  Fly  4993 SGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYH-KYTSKSAQIQ 5056
            ...:.||.:|.|.|||:::...|:|:|:.:||||:|:|..:||:.|.:.||||. .|......|:
Mouse  1448 ERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIR 1512

  Fly  5057 WFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQ 5121
            |||.|:..|:...|.:.||||||||.||.:||.:|.|.||:::|.|.:..:.|. ||.||||||:
Mouse  1513 WFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITS-LPRAHTCFNR 1576

  Fly  5122 LDLPMYKSYDKLRSCLLKAIHECSEGFG 5149
            ||||.|.||..|...||.|:.|.|. ||
Mouse  1577 LDLPPYPSYSMLYEKLLTAVEETST-FG 1603

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740	9/37 (24%)
		DUF4414	3675..3779	CDD:291075	24/130 (18%)
		HECTc	4796..5149	CDD:238033	143/354 (40%)
		HECTc	4820..5148	CDD:214523	137/328 (42%)
Hecw1	XP_006516872.1	HECW_N	66..185	CDD:374632	5/17 (29%)
		C2_NEDL1-like	206..342	CDD:176073	27/145 (19%)
		Neuromodulin	378..500	CDD:369001	28/127 (22%)
		WW	829..858	CDD:366073	7/33 (21%)
		HECW1_helix	947..1013	CDD:375862	14/80 (18%)
		WW	1019..1050	CDD:197736	8/33 (24%)
		HECTc	1249..1603	CDD:238033	143/356 (40%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810