DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and MAGI1

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	NP_001028229.1	Gene:	MAGI1 / 9223	HGNCID:	946	Length:	1462	Species:	Homo sapiens

Alignment Length:	1685	Identity:	297/1685 - (17%)
Similarity:	444/1685 - (26%)	Gaps:	708/1685 - (42%)

- Green bases have known domain annotations that are detailed below.

  Fly  2553 AAAPGSGVGGGGSGAGSVGGG-----VSG-SVSGNSSAAVIDVVEERS---------NGGDTNDP 2602
            ||...:|:.|||.|. .:|.|     |.| .|||.....|:.|::...         .||..|
Human    48 AAVEAAGLPGGGEGP-RLGEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLN-- 109

  Fly  2603 PANVSSASSGAGQSSTSGGP-------VR--------------------PVVNFDQLW-DDFLQS 2639
                ........|....|.|       :|                    |.|:::.|. .:||..
Human   110 ----KDLRHFLNQRFQKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYNFLTVKEFLDL 170

  Fly  2640 EG----LRLASRSGGGAGSG-------SGSV---GASSSGQQNGSNTTASSTNSGN---NSGIGN 2687
            |.    |.:.:..|...|:.       ||.|   .|..|.|.....:|...|.|.|   |:||
Human   171 EQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGI-- 233

  Fly  2688 LRLRTNPDVDVGSGNGSGSGSGSNAGVVNDNGISGGGGSDGASTSSDTGAPGGARDLNASLLGDV 2752
            :......:.||...|.|               .:...|.....|..:|..|    .:|:|::...
Human   234 VHAENEEEDDVPEMNSS---------------FTADSGEQEEHTLQETALP----PVNSSIIAAP 279

  Fly  2753 STSESDSDPS---------------------TENDE-----------------------RNEEDE 2773
            .|..|...|.                     |||.|                       :..|:.
Human   280 ITDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEEC 344

  Fly  2774 DEDDDAPDEDVDEHSETDVDEERSRQ---FIEVFDHI-----YEPESSETASNDADVDNDDDDDE 2830
            ::|:....|::|...|.....|:...   .|...|||     ||....| |.....::......:
Human   345 EDDEGVHTEELDSELELPAGWEKIEDPVYGIYYVDHINRKTQYENPVLE-AKRKKQLEQQQQQQQ 408

  Fly  2831 DDVDDDDDDDEDNDDDVREEVQSVMDAEIENRIDIALALVSSNNRPRRSAAVNASGDGSANRTNP 2895
            ..........:...::..|:..:::...|.|.       ..||..|.|...:    .|....|..
Human   409 QQQQQQQQQQQQQTEEWTEDHSALVPPVIPNH-------PPSNPEPAREVPL----QGKPFFTRN 462

  Fly  2896 DDEV-----------------------DDEDD---------DVDADPDGDPDVDP---------I 2919
            ..|:                       |:.|:         |..|..||..:...         :
Human   463 PSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCV 527

  Fly  2920 SGPSAAQAEAYLYERLGSGLMPTPPVGFRVRMNSSIAQPLDID---------------------V 2963
            .|.:.||. ..:::.:        |:|..|.:......||..|                     |
Human   528 LGHTHAQV-VKIFQSI--------PIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIV 583

  Fly  2964 NGGNA--GSGSNAGATGSSGGS-----------GQRSSTGGTTASIGAIASAALRPSSGSWMAPD 3015
            ||...  ...|::..||...|.           ...||.|....::...:|.|.:|
Human   584 NGQETYDSPASHSSKTGKVNGMKDARPSSPADVASNSSHGYPNDTVSLASSIATQP--------- 639

  Fly  3016 FNFIRISGGDSSMGSGPAIGGNANGGDMNVFITPSMSLERAGGAPGSGQTMSE------------ 3068
             ..|.:......||.|..|..:..||...|  ...:...|..|.. .|..:.|
Human   640 -ELITVHIVKGPMGFGFTIADSPGGGGQRV--KQIVDSPRCRGLK-EGDLIVEVNKKNVQALTHN 700

  Fly  3069 ----LLNEQPTGAGGGGGGGSASGVGGVGANGGTTGQHPNATLCTNGVRRRLLYLDQQYCV---- 3125
                :|.|.|.|          |.|..:...||.  ..|..:..:..:.|:......|:.|
Human   701 QVVDMLVECPKG----------SEVTLLVQRGGL--PVPKKSPKSQPLERKDSQNSSQHSVSSHR 753

  Fly  3126 ----CMPTHQN------PTEETQMETFTQ-------DALHWWLE-----EATLLDMESQ------ 3162
                ..|:|..      |..|.|....|.       |....|.:     |..|.|.:.|
Human   754 SLHTASPSHSTQVLPEFPPAEAQAPDQTDSSGQKKPDPFKIWAQSRSMYENRLPDYQEQDIFLWR 818

  Fly  3163 -------------------------------TDACLYAAHFLL-----PHLIKELKAAHQQRKQ- 3190
                                           ||..|.:...|:     |.:.|    :||...|
Human   819 KETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGK----SHQLVVQL 879

  Fly  3191 -EEAARQS---------------------TTPASGSNASATSSSTTSVVGTAAPTATSSIASGLL 3233
             ::||:|.                     .:|||..::|...:|.|....|...:..|
Human   880 MQQAAKQGHVNLTVRRKVVFAVPKTENEVPSPASSHHSSNQPASLTEEKRTPQGSQNS------- 937

  Fly  3234 ANAVSAGSGSS--------------SLVVPPVAASATGGTRRSSIPVLISSTLGPSSASSATSA- 3283
            .|.||:||||:              |.||.|.......|.......|::||...|.:.::..:|
Human   938 LNTVSSGSGSTSGIGSGGGGGSGVVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGNAC 1002

  Fly  3284 --------------------------------------------------TGNGAT--------- 3289
                                                              .||..|
Human  1003 VAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDES 1067

  Fly  3290 SDAT-----------TTTGGPTRNFSSVDSNVSSETQTTTRPPRQRGGLRQQSQLPFNIYAEIDL 3343
            |:||           |||..|::       ..:.||:.||:|       :|:||..|.   ....
Human  1068 SNATLLTNAEKIATITTTHTPSQ-------QGTQETRNTTKP-------KQESQFEFK---APQA 1115

  Fly  3344 TNEQD----------------SEGDRSTST---TTGTAADGAESQS----------QHNSSSTAA 3379
            |.|||                ..|.|..:.   ....|.||...:.          :.|..:|..
Human  1116 TQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKN 1180

  Fly  3380 QVAVPQHQRHPPVPLLPSSGRPSRPHTRLDETELLLLQLTDRQIRNRLQGSSGHTNSSRTSGSAL 3444
            .    :|.|  .:.|:.:.||..|          |.|:..|..:......|..|..::...|...
Human  1181 M----KHSR--AIELIKNGGRRVR----------LFLKRGDGSVPEYDPSSDRHGPATGPQGVPE 1229

  Fly  3445 INARPNRDLPACLQRHDHPA-GSIFPRPSANRTPAPTLVSMPTPMSNAALEQLNVTAPDVVRTDR 3508
            :.|.|:        |..||: .|.:|                               ||:.::.
Human  1230 VRAGPD--------RRQHPSLESSYP-------------------------------PDLHKSS- 1254

  Fly  3509 NFALPSSLEVDVDVLPGIVDLPQQEAQPEDDQLLLPPPPPPPPSDTTPLVHAEDDLWPGTVVSAG 3573
                                 |..|.:                      .||.|        ..|
Human  1255 ---------------------PHGEKR----------------------AHARD--------PKG 1268

  Fly  3574 TREESSAPAVATESTEAVNESNQPEPTPESSESTSPNPQAPSAEPTPLEPGAAVDASVGAQTPAT 3638
            :||.|..|          ||.:       :...||..|.:.:..|....|....||
Human  1269 SREYSRQP----------NEHH-------TWNGTSRKPDSGACRPKDRAPEGRRDA--------- 1307

  Fly  3639 TEEAASTAGASGTTATITDMSPEVRAALGDLEVPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQ 3703
              :|...|.|:|...    .|||.|.     |.....|.:.......|.|.:|..|. |.:|...
Human  1308 --QAERAAAANGPKR----RSPEKRR-----EGTRSADNTLERREKHEKRRDVSPER-RRERSPT 1360

  Fly  3704 RAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLP 3768
            |.:..:......||                 |.|::||...:|:  :..:||
Human  1361 RRRDGSPSRRRRSL-----------------ERLLEQRRSPERR--RGGSPE------------- 1393

  Fly  3769 ENLRQAILTDMEESQIASLPPELAAEAQFLRRDWESR-------------NGARMDAHPP 3815
               |:|..||...::    .||...|....:|:.|.|             :..|...|||
Human  1394 ---RRAKSTDRRRAR----SPERRRERSLDKRNREDRASHREREEANLKQDAGRSSRHPP 1446

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740	16/75 (21%)
		DUF4414	3675..3779	CDD:291075	20/103 (19%)
		HECTc	4796..5149	CDD:238033
		HECTc	4820..5148	CDD:214523
MAGI1	NP_001028229.1	PDZ_signaling	15..100	CDD:238492	16/52 (31%)
		GuKc	122..293	CDD:214504	38/191 (20%)
		WW	303..332	CDD:238122	4/28 (14%)
		WW	361..390	CDD:278809	7/28 (25%)
		PDZ	469..555	CDD:214570	12/94 (13%)
		PDZ	640..723	CDD:214570	18/95 (19%)
		MAGI_u5	722..805	CDD:293271	15/84 (18%)
		PDZ_signaling	819..894	CDD:238492	12/78 (15%)
		PDZ_signaling	968..1062	CDD:238492	10/93 (11%)
		PDZ	1123..1205	CDD:214570	15/97 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.900