DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and Nedd4l

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	XP_036017204.1	Gene:	Nedd4l / 83814	MGIID:	1933754	Length:	1261	Species:	Mus musculus

Alignment Length:	1564	Identity:	379/1564 - (24%)
Similarity:	590/1564 - (37%)	Gaps:	473/1564 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly  3733 QSEVLMQQRIEQQRQAAQT----ANPEDPVDTAAFFQNLPEN--LRQAILTDMEESQIASLP--- 3788
            :||:|.|:|.|....|.||    ::|.      :|.|:..|:  |::.: ..::.|....:|
Mouse    19 ESEILDQEREEDFFMAFQTLPRRSSPH------SFTQSTGEDSGLQEGV-GALKRSSSMFIPQLL 76

  Fly  3789 ------PELAAEAQF-LRRDWESRNGARMDAHPPSNALSRFQTTLQSLDD----TRWHSSIW--- 3839
                  |..::..|. |:|  ::.:||.....||.....|..:|...|.|    ||..:.|.
Mouse    77 ARVDARPTRSSSVQISLQR--KAADGATDSCGPPEGPEDRPPSTQADLGDQAAATRVSAQISSSE 139

  Fly  3840 ---YDASGNAQPQ--------------HHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDP 3887
               .|..|::.|:              ||      .|.||....|....|...:.....:.
Mouse   140 PSPVDTPGSSPPRAAWDPGTLVNGTCDHH------GQCPHLAEGPEEATPGLRIQHRASSA---- 194

  Fly  3888 DSLATLLLFLFVEDQKVNSMRLHRVIRNLCHHEPTRDWIIS------------ALISIIQKTNED 3940
             .:..:.|..|..|.:.               ||.| |.:.            ..:..:|:..:.
Mouse   195 -DVRQVRLLPFGPDGQA---------------EPRR-WSLQHVPDASGSSGKRCFVFQLQQPQQG 242

  Fly  3941 ----NANFGAGQAGGKPEWL----KLRVDAAFGYKSNIFLINRLYEGSARSISINPQAAQMIVRN 3997
                ..:|..|..|.|.:.|    ::|::.:..|.:.    .|....|.......|:|:....|.
Mouse   243 ALGLTGDFNFGFTGTKGDRLVRYPRIRLERSTSYPTQ----PRAGRVSPTEDRGTPEASPRASRV 303

  Fly  3998 CLDLLFVLAKHYPSS---FVPFNRDVKAR----RILSAVFGMPSP-----TAGSGGGAGSGSAAS 4050
            ..::....::..|.|   .....:|..|.    |||.......:|     |..|.|..|:|:...
Mouse   304 VPEIRRTNSERTPQSPGCMFKIRQDQNAGQQHFRILVTRGPEEAPQNQEETHKSPGHPGAGAPTR 368

  Fly  4051 GGLTGYDDVPPGGTLDFRSRFCRYQVVNRLRSIFDDHPAPKPSVPLDEAPST---------SKAA 4106
            ....|..|||                ::.|       |...|::   |.|.|         |..:
Mouse   369 DDFLGQVDVP----------------LSHL-------PTEDPTM---ERPYTFKDFLLRPRSHKS 407

  Fly  4107 AAKAAATAKVS---TAGSQNKHSVPYQTNAKNFWDVVLNID--QQTQVRLANVAQFPLTSQPAWE 4166
            ..|.....|::   ..|.|::.:...:.:.::.|:||.:.|  .|.|..|.     |....|.||
Mouse   408 RVKGFLRLKMAYMPKNGGQDEENSEQRDDMEHGWEVVDSNDSASQHQEELP-----PPPLPPGWE 467

  Fly  4167 --------------------W-QPNTADFLSFSESPFGQLLEMLPYKVICRSPHLTDMLVKLLAS 4210
                                | :|:..|..|.|::...|:.:...::......|:::.|....:.
Mouse   468 EKVDNLGRTYYVNHNNRSTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEASE 532

  Fly  4211 LSTELPKEEELLPLSDQMPPLVPIGPVQRAGAAAAAAHPPPPQPPAENTATATEIGDGLAANIE- 4274
            ...|.|:..|  .:|::|         ..||.:.:.|.||||..|...| :..|:.:.::..::
Mouse   533 GGGEGPEPWE--TISEEM---------NMAGDSLSLALPPPPASPVSRT-SPQELSEEVSRRLQI 585

  Fly  4275 ------EQAS------PASGNPASGDSNAGAKRTQLPTLSVALPVTPSKPRRKIYSKNFSQLQLV 4327
                  ||.|      |:|...:...::..|::..||        .||.|.|:..|...:
Mouse   586 TPDSNGEQFSSLIQREPSSRLRSCSVTDTVAEQAHLP--------PPSTPTRRARSSTVT----- 637

  Fly  4328 IEVLTHQCGTTEGLDNVAKLIVNLTQCSQASSAIFIQYLTAAILGLAEEVRQAIQMLLNEIRMYN 4392
                    |..|...:||.:                 :.|..:....||.:.|      :.|.|.
Mouse   638 --------GGEESTPSVAYV-----------------HTTPGLPSGWEERKDA------KGRTYY 671

  Fly  4393 IHQGGAGTT-----SQAASTSGAAAATASYVPTVSGASLLPNLAVHEGIMQDR-FTAEHVIISAP 4451
            ::.....||     .|.|....:.:||.|           .|..|...|.:.| .::..|.:|||
Mouse   672 VNHNNRTTTWTRPIMQLAEDGASGSATNS-----------NNHLVEPQIRRPRSLSSPTVTLSAP 725

  Fly  4452 KNAKPTCELQLPSMKKLMSN-SSAQPYFLRTLKIFMQVRDMYEAQNGLSTGGGVACGAGDNDDDI 4515
            ........:: .::|..:|| .|.||....:.|
Mouse   726 LEGAKDSPIR-RAVKDTLSNPQSPQPSPYNSPK-------------------------------- 757

  Fly  4516 IDIEGDGGASGGSSSDMAPGSASTISPPPPMETNQIAQDGDDDEEDDDEARSFPGVGNHAVASAS 4580
                              |....|.|..||....:||.:|          |.|         ...
Mouse   758 ------------------PQHKVTQSFLPPGWEMRIAPNG----------RPF---------FID 785

  Fly  4581 KSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFM 4645
            .:||.|         .|          |:.:.:|.|                    ..:|..|..
Mouse   786 HNTKTT---------TW----------EDPRLKFPV--------------------HMRSKASLN 811

  Fly  4646 PNAAGFGLRAAAGAGAIGGLGAAAPSAQEY-----SPFMDDVSSA------NTPSSASFDD---- 4695
            ||                .||...|..:|.     ..|..|.||.      :|..|....|
Mouse   812 PN----------------DLGPLPPGWEERIHLDGRTFYIDHSSQVLCGENDTRESVPLYDSKIT 860

  Fly  4696 ---EPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDG 4757
               :||:|:     |.:|.|:
Mouse   861 QWEDPRLQN-----PAITGPA-------------------------------------------- 876

  Fly  4758 PFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSF-RVLYRLGPEEWKN 4821
                 |.::|  :|..|..||:.:|::..:...|.|  :.:.|..:||:|: |::....|:..|.
Mouse   877 -----VPYSR--EFKQKYDYFRKKLKKPADIPNRFE--MKLHRNNIFEESYRRIMSVKRPDVLKA 932

  Fly  4822 RFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSH-ANPNHLSYFK 4885
            |.:|.||.|:|.|.||:.|||:.::|:|:|||.|.||..|..|..|..|||:|. .|.:|||||.
Mouse   933 RLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFT 997

  Fly  4886 FVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELT 4950
            |:|||...||...|||:.:|.|.|||.:||||:...||||.|.|:|..|.::::||.:.|  :|.
Mouse   998 FIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTEL--DLM 1060

  Fly  4951 FSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHL 5015
            |..:.:.||.|...||||||.:..||.|||.||:.||.|.:....:::|::||||||.:::|..|
Mouse  1061 FCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDL 1125

  Fly  5016 ISIFNEQELELLISGLPDIDIEDLKANTEY-HKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTG 5079
            |.||:|.|||||:.||.|:|:.|.:.::.| :.|......|||||:|:...|...|.:.||||||
Mouse  1126 IKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTG 1190

  Fly  5080 TSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHEC 5144
            ||:||:.||..|.|.||.|.|.|.:.. |.::||.||||||:||||.|::::.||..||.|: |.
Mouse  1191 TSRVPMNGFAELYGSNGPQLFTIEQWG-SPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAV-EN 1253

  Fly  5145 SEGF 5148
            ::||
Mouse  1254 AQGF 1257

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075	14/51 (27%)
		HECTc	4796..5149	CDD:238033	164/356 (46%)
		HECTc	4820..5148	CDD:214523	156/329 (47%)
Nedd4l	XP_036017204.1	PHA03378	<107..318	CDD:223065	40/241 (17%)
		C2	<367..420	CDD:417471	13/78 (17%)
		WW	465..491	CDD:395320	3/25 (12%)
		WW	655..684	CDD:395320	7/34 (21%)
		WW	768..798	CDD:238122	12/67 (18%)
		WW	817..867	CDD:197736	10/49 (20%)
		HECTc	928..1257	CDD:214523	156/332 (47%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810