Protein Alignment HUWE1 and itch

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: NP_001072737.1
• Gene: itch / 780194
• XenbaseID: XB-GENE-976485
• Length: 853
• Species: Xenopus tropicalis
• Alignment Length: 814
• Identity: 235/814 - (28%)
• Similarity: 358/814 - (43%)
• Gaps: 154/814 - (18%)

  Fly  4429 NLAVHEGIMQDRFTAEHVIISAPKNAKPTCELQLPSMKKLMSNSSAQPYFLRTLKIFMQVRDMYE 4493
            :|.:||.::.:....|.||::          :||.|.::.:.:.|.             ..|..:
 Frog    99 SLNLHETLLSNNMKLEEVIVT----------MQLLSDRETVGDLSV-------------CLDGMQ 140

  Fly  4494 AQNGLSTGGGVACGAGDNDDDIIDIEGDGGA---------------SGGSSSDMAPGSASTISPP 4543
            ....|...|..|..:|..|:...::|....:               :|.||..:|.|......||
 Frog   141 VDPELLANGDPAVSSGSTDNSSTEVESQAASAESSNGTNSTDSRTTNGMSSPSLANGGFKASRPP 205

  Fly  4544 ----PPMETNQIAQD-----GDDDEEDDDEARSFPGVGNHAVASASKSTKPTLSQILCLDV---- 4595
                ||..|.:.|..     .:.....:.|....|...:...|.|..||.|...||..::|
 Frog   206 RPSRPPPPTPRRASGVVTAISEAPPPPEAELLPTPATASLPEAQAPSSTPPVSRQIAPINVDGQR 270

  Fly  4596 ---------------------------------LWNTLSECLVALEESKDEFAVLVL-------- 4619
                                             .|:.........|...|....:..
 Frog   271 MGLVNQGPLPPGWEQRVDQQGRVYYVDHVAKRTTWDRPEPLPPGWERRVDNMGRIYYVDHITRTT 335

  Fly  4620 ---QPTVEA------FFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEY 4675
               :||:|:      :.|.....:||.::..:.|:                .|.........:||
 Frog   336 TWQRPTLESVRNYEQWQLQRNQLQGAMQQFNQRFI----------------YGTQETGTTQNKEY 384

  Fly  4676 SPF----------MDD---VSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQ 4727
            .|.          ||.   |...|..:..:..::||.|......||         ....|.:...
 Frog   385 DPLGPLPVGWEKRMDGNGRVYFVNHTTRTTQWEDPRNQGQLNEKPL---------PEGWEMRFTV 440

  Fly  4728 DRQKFLQFAEKHRTVLNQI-LRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRR 4791
            |...:  |.:.:|.....| .|...:.|.:||....|     .||..|.:||:...::|    ..
 Frog   441 DGIPY--FVDHNRKTTTYIDPRTGKSALDNGPHIAYV-----RDFKAKVQYFRFWCQQL----YM 494

  Fly  4792 EEHT-VSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMY 4855
            .:|. ::|.|.|:|||||:.:.....::.:.|.:|:...|||.|.||:.|||:.::|.|:.||||
 Frog   495 PQHVKITVNRKTLFEDSFQQIMSFNAQDLRRRLWIIIPGEEGLDYGGVAREWFFLLSHEVMNPMY 559

  Fly  4856 ALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKH 4920
            .||..:..|.....|||:|:.||:||.||:|:||.||.|:...|.::..|:..|||.||.|.|..
 Frog   560 CLFEYAGKDNYCLQINPASYINPDHLRYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGL 624

  Fly  4921 TDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQ 4985
            .|:||.|.|||..|.::..|:|...|.|:.||.:.:..|..:..||||:|.:..||||||.||::
 Frog   625 KDLESVDPEFYNSLIWIKDNNIEECGLEMFFSVDKEILGDVKSHDLKPDGSNIQVTEENKEEYIR 689

  Fly  4986 LVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTS 5050
            ||.:.::|..:.:|..||.|||.:|:|:..:..|:.:|||:|:.|:.:||:.|.:.||.|..||.
 Frog   690 LVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRNTIYRHYTR 754

  Fly  5051 KSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCA 5115
            .|.||.|||:.::..|...|.:.|||||||.::|:.||..|.|.||.|||.|.:..:. :.||.:
 Frog   755 TSKQIIWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKE-NWLPRS 818

  Fly  5116 HTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5149
            |||||:||||.||||::|:..||.||.| :||||
 Frog   819 HTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 851

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159
		HECTc	4796..5149	CDD:238033	155/352 (44%)
itch	NP_001072737.1	C2_E3_ubiquitin_ligase	17..137	CDD:175988	10/60 (17%)
		WW	279..308	CDD:459800	1/28 (4%)
		HUL4	<310..853	CDD:227354	196/580 (34%)