Protein Alignment HUWE1 and HACE1

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: NP_065822.2
• Gene: HACE1 / 57531
• HGNCID: 21033
• Length: 909
• Species: Homo sapiens
• Alignment Length: 978
• Identity: 265/978 - (27%)
• Similarity: 414/978 - (42%)
• Gaps: 205/978 - (20%)

  Fly  4293 AKRTQLP-----TLSVALPVTPSKPRRKI--------YSKN--FSQLQLVIEVLTHQCGTTEGLD 4342
            |:..:||     .:...:|:..:...|.:        :..|  |.:::..:..:...||:.|.|.
Human    18 ARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLV 82

  Fly  4343 NVAKLIVN-----LTQCSQASSAI----------FIQY-----------LTA----AILGLAEEV 4377
            .:.|...|     ::.|:....|.          .::|           |||    |:.|..|.:
Human    83 LLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELL 147

  Fly  4378 RQAIQML--------LNEIRMYNIHQGGAGTTSQAASTSGAAAATASYVPTVSGASLLPNLAVHE 4434
            ...:|.:        :.:..::...|.|..||.|....|||....    |.||||:.|.....|
Human   148 HDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINR----PNVSGATPLYFACSH- 207

  Fly  4435 GIMQDRFTAEHVIISAPK------NAKP-----------TCELQL-------PSMKKLMSNSSAQ 4475
               ..|.||:.:::...|      ...|           |||:.:       .::.::..|...:
Human   208 ---GQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLR 269

  Fly  4476 PYFLRTLKIFMQVRDMYEAQNGLSTGGGVACGAGDNDDDIIDIEGDGGASGGSSSDMAPGSAST- 4539
            ...||  ::...:....|:|. |.....:|..|..|...::.:..:..|...|...:.......
Human   270 ENMLR--QVLEHLSQQSESQY-LKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVF 331

  Fly  4540 -ISPPPPMETNQIAQDGDDDEEDDDEARSFPGVGNHAVASASKSTKPTLSQILCLDVLWNTLSEC 4603
             |.|..|.....:..:|:.                   ...|:..||       |::||::|.|.
Human   332 RIGPSSPSNGIDMGYNGNK-------------------TPRSQVFKP-------LELLWHSLDEW 370

  Fly  4604 LVAL-------EESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGA 4661
            ||.:       :....|...::|                  |:.|:.  .:||........|.|:
Human   371 LVLIATELMKNKRDSTEITSILL------------------KQKGQD--QDAASIPPFEPPGPGS 415

  Fly  4662 IGGLGA----AAPSA----QEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAM 4718
            ...|..    :.|.|    ||.|....||.|......::......|..:....|.:|:|
Human   416 YENLSTGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSP------ 474

  Fly  4719 SAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDH-----------TRILDFD 4772
                        :|::|..||..||...:.::|        .::.||           :|.:..
Human   475 ------------RFIEFVCKHDEVLKCFVNRNP--------KIIFDHFHFLLECPELMSRFMHI- 518

  Fly  4773 VKRKYFQTELERLDEGIR-------------REEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFY 4824
            :|.:.|:...|...|.:.             .|...:.|.|.::|..|..|:.:....:.|....
Human   519 IKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIA 583

  Fly  4825 IVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGR 4889
            :.|..|||. ..|::|||:.|:|.||.||.||||..| .|..|:..|.:|:.||:||:||:|.|:
Human   584 VRFHGEEGM-GQGVVREWFDILSNEIVNPDYALFTQS-ADGTTFQPNSNSYVNPDHLNYFRFAGQ 646

  Fly  4890 VIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTE 4954
            ::..|::..:|:..||||||||||||..|.:.|:.|.|.|:.|.|.:::.||||.||.|||||.|
Human   647 ILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVE 711

  Fly  4955 VQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIF 5019
            ...||..:...|||.|....||:.||.||||||.:|:|:.:|:.|::|||:||:..||..||.:|
Human   712 TDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLF 776

  Fly  5020 NEQELELLISGLPDIDIEDLKANTEYHK-YTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKV 5083
            :|.|||||:||:|:||:.|...||||.. |..:...|||||..:....|.:|...||||||:|:|
Human   777 DEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRV 841

  Fly  5084 PLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGF 5148
            |..||.::.|.:|:|.|.|.....:.:.||.:.||.|.|.||.|.|.:.|:..||.|:|..|.|:
Human   842 PHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGY 906

  Fly  5149 GFA 5151
            ..|
Human   907 TMA 909

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159
		HECTc	4796..5149	CDD:238033	157/353 (44%)
HACE1	NP_065822.2	N-terminal helix important for homodimerization. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367	1..21		1/2 (50%)
		ANK 1. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367, ECO:0007744|PDB:8H8X, ECO:0007744|PDB:8HAE, ECO:0007744|PDB:8PWL	23..55		4/31 (13%)
		ANKYR	26..249	CDD:440430	43/230 (19%)
		ANK 2. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367, ECO:0007744|PDB:8H8X, ECO:0007744|PDB:8HAE, ECO:0007744|PDB:8PWL	64..93		6/28 (21%)
		ANK repeat	66..95	CDD:293786	6/28 (21%)
		ANK repeat	97..128	CDD:293786	3/30 (10%)
		ANK 3. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367, ECO:0007744|PDB:8H8X, ECO:0007744|PDB:8HAE, ECO:0007744|PDB:8PWL	97..126		3/28 (11%)
		ANK repeat	130..161	CDD:293786	7/30 (23%)
		ANK 4. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367, ECO:0007744|PDB:8H8X, ECO:0007744|PDB:8HAE, ECO:0007744|PDB:8PWL	130..159		7/28 (25%)
		ANK repeat	163..194	CDD:293786	8/34 (24%)
		ANK 5. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367, ECO:0007744|PDB:8H8X, ECO:0007744|PDB:8HAE, ECO:0007744|PDB:8PWL	163..192		8/28 (29%)
		ANK 6. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367, ECO:0007744|PDB:8H8X, ECO:0007744|PDB:8HAE, ECO:0007744|PDB:8PWL	196..226		9/33 (27%)
		ANK repeat	196..221	CDD:293786	8/28 (29%)
		ANK 7. /evidence=ECO:0000269|PubMed:37537642, ECO:0000269|PubMed:38332367, ECO:0007744|PDB:8H8X, ECO:0007744|PDB:8HAE, ECO:0007744|PDB:8PWL	228..253		4/24 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	398..433		7/36 (19%)
		HECTc	554..903	CDD:238033	155/350 (44%)