Protein Alignment HUWE1 and wwp2

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: XP_005163228.1
• Gene: wwp2 / 564527
• ZFINID: ZDB-GENE-000607-82
• Length: 867
• Species: Danio rerio
• Alignment Length: 1000
• Identity: 269/1000 - (26%)
• Similarity: 418/1000 - (41%)
• Gaps: 257/1000 - (25%)

  Fly  4270 AANIEEQASPASGNPASGDSNAGAKRTQLPTLSV--ALPVTPSKPRRKIYSKNFSQLQLVIEVLT 4332
            ||::.:.||..:           .:::|| ||.|  |.|.:|..|.|      .|:|...:||
Zfish     3 AASVSQSASIQA-----------IEKSQL-TLKVLSAKPQSPKLPNR------HSRLSSFVEV-- 47

  Fly  4333 HQCGTTEGLDNVAK----------------LIVNLTQCSQASSAIFI------QYLTAAILGLAE 4375
                |.:||.:..|                |.:|:|..|:....::.      :.|.:|.:.|.:
Zfish    48 ----TADGLSSETKKTGKRSGHLELQWNEDLTLNVTPQSRLDLKVWSCHTLRKELLGSATIDLLD 108

  Fly  4376 EVRQAIQMLLNEIRMYNIHQGGAGTTSQAASTSGAAAATASYVPTVSGASL---LPNLAVHEGIM 4437
            .:|      .::.:|.|:.......|....|             .|:|..|   |..|.|..|.:
Zfish   109 TLR------THDGKMENVQLSLVLQTENKGS-------------VVAGGELNVCLDGLVVDLGSL 154

  Fly  4438 QDRFTAEHVIISAPKNAKPTCELQLPSMKKLMSNSSAQPYFLRTLKIFMQVRDMYEAQNGLSTGG 4502
            .:...|...|.|:..:.......:.|        |::.|          :.|.:.::..| |.|.
Zfish   155 PNGSAASDKIHSSRSSLHRAHSEETP--------STSSP----------RGRSLGQSSRG-SCGD 200

  Fly  4503 ------GVACGAGDNDDDIIDI-----------------EGDGGASGGSSSDM---APGSAS--- 4538
                  |.|...||.:..:.:.                 :|:..|:|..|:.:   ||.|.|
Zfish   201 SPAEIQGAALTNGDVNGALAETTSRHNSKTTVNNLSSAGKGETAANGLESAHLTCPAPSSPSLSS 265

  Fly  4539 -----TISPP---PPMETNQIAQDGDDDEEDDD------EARSFPGVGNHAVASASKST---KPT 4586
                 |:.||   ||..|.|  .|...|.::.|      |.|..|....:.|...:|:|   :|
Zfish   266 NPPDGTVPPPAAEPPASTEQ--SDTTSDTQNTDALPAGWEQRILPHGRVYYVDHNTKTTTWERP- 327

  Fly  4587 LSQILCLDVLWNTLSECLVALEESKDEFAVLVL-----------QPTVEAF--FLIHASHR---- 4634
                  |...|          |:..|:......           :||.|:.  :....|.|
Zfish   328 ------LPPGW----------EKRVDQRGRFYYVDHNTRTTTWQRPTAESVRNYEQWQSQRSQLQ 376

  Fly  4635 GASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRM 4699
            ||.::..:.::...:|    |......:|.|    |...|.......|...|..:..:..::||.
Zfish   377 GAMQQFNQRYLYQPSG----AVVENDPLGAL----PPGWEKRQDNGRVYYVNHNTRTTQWEDPRT 433

  Fly  4700 QDASTNTPL----------------VTNPSTSTAMS----AHEAQLRQD----------RQKFLQ 4734
            |......||                |.:.|.:|...    ..|:..||.          |.|:.|
Zfish   434 QGMIQEPPLPPGWEMKYTAEGVRYFVDHNSRTTTFKDPRPGFESGSRQGGSPGAYDRSFRWKYHQ 498

  Fly  4735 FAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVR 4799
            |.                         .:.|:..|...||                     :||.
Zfish   499 FR-------------------------FLCHSNALPSHVK---------------------ISVS 517

  Fly  4800 RVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGD 4864
            |.|:|||||:.:..:.|.:.:.|.||:...|||.|.||:.|||:.::|.|:.||||.||..:..:
Zfish   518 RQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKN 582

  Fly  4865 RVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYE 4929
            .....|||:|..||:||:||:|:||.||.|::..|.::..||..|||.:|.|:....|:||.|.|
Zfish   583 NYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKKPTLKDLESIDPE 647

  Fly  4930 FYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSG 4994
            ||..:.::.:||:...|.||.|:.:::..|......||.:|.:..||::||.||:.|:...:.:.
Zfish   648 FYNSIMWVKENDLEECGVELYFAQDMEILGKVTTHQLKDDGENELVTQDNKEEYIGLLTDWRFTR 712

  Fly  4995 SIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFW 5059
            .:.:|..|||:||.:::|...:..|:|:||||::.|:.:||:.|.:.||.|..||..|.||.|||
Zfish   713 GVEEQTKAFLDGFNEVVPLEWLRYFDEKELELMLCGMQEIDLNDWQKNTIYRHYTKNSKQIHWFW 777

  Fly  5060 RALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDL 5124
            :.::..|...|.:.|||||||.::|:.||..|.|.||.|||.|.:..:.| .||.:|||||:|||
Zfish   778 QVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKET-WLPRSHTCFNRLDL 841

  Fly  5125 PMYKSYDKLRSCLLKAIHECSEGFG 5149
            |.||:.::||..||.||.| :||||
Zfish   842 PPYKNLEQLREKLLFAIEE-TEGFG 865

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159
		HECTc	4796..5149	CDD:238033	149/352 (42%)
wwp2	XP_005163228.1	C2_E3_ubiquitin_ligase	17..145	CDD:175988	33/159 (21%)
		WW	298..327	CDD:459800	6/28 (21%)
		WW	328..357	CDD:459800	4/38 (11%)
		WW	403..431	CDD:459800	5/31 (16%)
		WW	441..473	CDD:197736	5/31 (16%)
		HECTc	513..865	CDD:238033	150/374 (40%)