DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and smurf2

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	NP_001107898.1	Gene:	smurf2 / 563633	ZFINID:	ZDB-GENE-030131-1830	Length:	765	Species:	Danio rerio

Alignment Length:	459	Identity:	189/459 - (41%)
Similarity:	268/459 - (58%)	Gaps:	25/459 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly  4696 EPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFA 4760
            :||:   |.|..||.|||.:.:.:|.||   |...:..|..|:.::|:      ||.:|.:.|..
Zfish   327 DPRL---SANLHLVLNPSPNGSRAAVEA---QSSSRPGQLKEQAQSVV------SPGNLPEDPEC 379

  Fly  4761 VLVDHTRILDFDVKRKYFQTELERLDEGIRREEH-TVSVRRVTVFEDSFRVLYRLGPEE-WKNRF 4823
            :.|...: .|...|.|..:.||.:...   :..| .:.|.|..:||:|:|.:.::.|:: || |.
Zfish   380 LTVPKYK-RDLVQKLKILRQELSQQQP---QAGHCRIEVSREEIFEESYRQVMKMRPKDLWK-RL 439

  Fly  4824 YIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVG 4888
            .:.|..|||.|.||:.|||..::|.|:.||.|.||..|..|..|..|||.|..||.|||||.|||
Zfish   440 MVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVG 504

  Fly  4889 RVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFST 4953
            |::..||.....::..||..|||.:|||.:...||||.|.:.:..|.:::.|||:.: .:.||..
Zfish   505 RIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGV-LDHTFCV 568

  Fly  4954 EVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISI 5018
            |...:|.....:|||||:...||::.|.|||:|....:....|..|..|..:||.::||:||:..
Zfish   569 EHNAYGEIIQHELKPNGKSIPVTQDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKA 633

  Fly  5019 FNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKV 5083
            |:|:||||::.||..|||.|.|:||.....|..|..::|||||:.|:|:..||:.||||||:|:|
Zfish   634 FDEKELELIVCGLGKIDINDWKSNTRLKHCTPDSNIVKWFWRAVESYDEERRARLLQFVTGSSRV 698

  Fly  5084 PLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHE-CSEG 5147
            |||||.:|:|..|.:.|.||:.|.||:.||.||||||::|:|.|:|||||...||.||.| |
Zfish   699 PLQGFKALQGAAGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYESYDKLYDKLLTAIEETC--- 760

  Fly  5148 FGFA 5151
             |||
Zfish   761 -GFA 763

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075
		HECTc	4796..5149	CDD:238033	159/354 (45%)
		HECTc	4820..5148	CDD:214523	151/328 (46%)
smurf2	NP_001107898.1	C2_Smurf-like	13..137	CDD:176028
		WW	159..188	CDD:278809
		WW	252..283	CDD:197736
		WW	298..330	CDD:197736	1/2 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	341..375		11/42 (26%)
		HECTc	410..762	CDD:238033	159/357 (45%)
		HECTc	437..762	CDD:214523	151/330 (46%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810