DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and ube3b

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	NP_001108154.1	Gene:	ube3b / 556864	ZFINID:	ZDB-GENE-060526-231	Length:	1069	Species:	Danio rerio

Alignment Length:	468	Identity:	149/468 - (31%)
Similarity:	219/468 - (46%)	Gaps:	63/468 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly  4725 LRQDRQKFLQFAE----KHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERL 4785
            ||:|.:..|.|.|    |.|.   |:|.|...|       |:....|:|.|   |.....|.|.|
Zfish   618 LRKDLKPSLLFQELEKGKKRA---QLLLQYIPH-------VIPHKNRVLLF---RNIVTKEKETL 669

  Fly  4786 D--EGIRREEHT--VSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVF-----EDEEGQDAGGLLRE 4841
            .  |......|.  :::||..:.||.:..|.||.....|....:.|     .||.|.|..|:.:|
Zfish   670 GLVETSSASPHVTHITIRRSRMLEDGYDQLRRLPVNSIKGVIRVKFVNDLGVDEAGIDQDGVFKE 734

  Fly  4842 WYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFT 4906
            :...|.:::|||...||..:.|:...|. :|:|..:.|||..|:|||:::.||:::..:::..|.
Zfish   735 FLEEIIKKVFNPALNLFKTTSGNERLYP-SPTSSIHENHLQLFEFVGKMLGKAMYEGIVVDVPFA 798

  Fly  4907 RSFYKHILGKQVKHT-------DMESQDYEFYKGLDYLMK--NDISTLGYELTFSTEVQEFGVTQ 4962
            ..|...:||..  |:       ::.|.|.||||.|..:.:  .|:|.||..|::..||  .|...
Zfish   799 SFFLSQVLGHH--HSTFYSSIDELPSLDSEFYKNLTSIKRYDGDVSDLGLTLSYDEEV--MGQLV 859

  Fly  4963 IRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELL 5027
            ..:|.|.|:...||.|||..|:.|:...:|...|::|..||:.||..||....:.:|:..|::.|
Zfish   860 CHELIPGGKTMPVTNENKISYIHLMAHFRMHTQIKEQTAAFIRGFRSIINPEWLHMFSTPEVQRL 924

  Fly  5028 ISG-LPDIDIEDLKANTEYH-KYTSKSAQIQWFWRALRS-FDQADRAKFLQFVTGTSKVPLQGFG 5089
            ||| ..:||::|||.:|.|: .:.|....|.|.|..|.| |...:||.||:|||..|:.||.||.
Zfish   925 ISGDNAEIDLDDLKKHTVYYGGFHSSHRVILWLWDILSSDFTPDERAMFLKFVTSCSRPPLLGFA 989

  Fly  5090 SLEGMNGIQKFQIHRDDRSTD-------------------RLPCAHTCFNQLDLPMYKSYDKLRS 5135
            .|:....|:..::..|..:.|                   |||.:.||||.|.||.|.....||.
Zfish   990 YLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKKEPGGRLPTSSTCFNLLKLPNYSKKSILRD 1054

  Fly  5136 CLLKAIHECSEGF 5148
            .|..|| ..:.||
Zfish  1055 KLRYAI-SMNTGF 1066

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075
		HECTc	4796..5149	CDD:238033	127/389 (33%)
		HECTc	4820..5148	CDD:214523	118/363 (33%)
ube3b	NP_001108154.1	IQ	28..50	CDD:197470
		HECTc	683..1067	CDD:238033	127/390 (33%)
		HECTc	708..1066	CDD:214523	118/363 (33%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810