DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and NEDD4

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	NP_001271267.1	Gene:	NEDD4 / 4734	HGNCID:	7727	Length:	1319	Species:	Homo sapiens

Alignment Length:	1315	Identity:	319/1315 - (24%)
Similarity:	522/1315 - (39%)	Gaps:	369/1315 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly  4076 VVNRLRSIFDDHPAPKPSVPLDEAPSTSKAAAAKAAATAKVSTAGSQNKHSVPYQTNAKNFWDVV 4140
            |.|.|.::.|.|....|::            .:.:.:....|..||       |.:|..:|....
Human   128 VDNELNAVNDGHLVSSPAI------------CSGSLSNFSTSDNGS-------YSSNGSDFGSCA 173

  Fly  4141 LNIDQQTQVRLANVAQFPLTSQPAWEWQPNTADFLSF---SESPFGQLL---EMLPYKVICRS-- 4197
                  :.....:.....::...::.:.|:...||..   |:|...:.:   ...|.::..||
Human   174 ------SITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTS 232

  Fly  4198 --PHLTDML------VKLLASLSTELPKEEELLPLSDQMPPLVPIGPVQRAGAAAAAAHPPPPQP 4254
              |.:.|.|      :||..|.:|::                    |::|..:.......|....
Human   233 TDPFVQDDLEHGLEIMKLPVSRNTKI--------------------PLKRYSSLVIFPRSPSTTR 277

  Fly  4255 PAENTATATEIGDGLAANIEEQ--ASPAS-GNPASGDSNAGAKRTQLPTLSVALPV-TPSKPR-- 4313
            |...|:..|.:..| :.....|  .||:. .:...|.|..|...|.:..|.::..: ||.:.|
Human   278 PTSPTSLCTLLSKG-SYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDI 341

  Fly  4314 RKIYSKNFSQLQLVI---------------------------EVLTHQCGTTEGLDNVAKLIVNL 4351
            |.::.|...|.::|:                           :..|..|.||.|   ..|..::.
Human   342 RPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNG---DCKPEMSE 403

  Fly  4352 TQCSQASSAIFIQYLTAAILGLAEEVRQAIQMLLNEIRMYNIHQGGAGTTSQAASTSGAAAATAS 4416
            .:.:..|..|.:.:.|:|.|..::.|...|.:.....|.::......|...::.|..||
Human   404 IKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGA------ 462

  Fly  4417 YVPTVSGASLLPNLAVHEGIMQ--DRFTA--EHVIISAPKNAKPTCELQLPSMKKLMSNSSAQ-- 4475
             .|.:|..|.|.:.....|..|  .:|.:  |..:.:..:::...|..:|.:..|...:...|
Human   463 -YPNISCLSSLKHNCSKGGPSQLLIKFASGNEGKVDNLSRDSNRDCTNELSNSCKTRDDFLGQVD 526

  Fly  4476 ---------------PYFLR-----------TLKIFMQVRDMYEAQNGLSTGGGVACGAGDNDDD 4514
                           ||..:           .:|.:::::..|..:.           :|..||:
Human   527 VPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKT-----------SGSEDDN 580

  Fly  4515 IIDIEGDGGASGGSSSDMAPG-----------------SASTISP-------------------- 4542
            ....|           ::.||                 ..|.:.|
Human   581 AEQAE-----------ELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESR 634

  Fly  4543 ------PPPMETNQIAQDGD----------------------DDEED--------DDEA-----R 4566
                  |.|.:....|::|:                      |:.|.        :|||     :
Human   635 RTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQ 699

  Fly  4567 SFPG-----------------------VGNHAVASASKSTKP----------TLSQILCLDVLWN 4598
            :||.                       .||.||:..:.|:..          |..:...|.||..
Human   700 AFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLP 764

  Fly  4599 TLSECLVALEESKDE-----------FAVLVLQPTVEAFFLIHASHRGASKKSG----------- 4641
            |.|......||.:||           ......:|||:|  .:..|...:|:.|.
Human   765 TSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQA--TVETSQLTSSQSSAGPQSQASTSDS 827

  Fly  4642 -----------RSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDD 4695
                       :.|:|.  |:.:|             .||:.:   ||..|.::..|    :::|
Human   828 GQQVTQPSEIEQGFLPK--GWEVR-------------HAPNGR---PFFIDHNTKTT----TWED 870

  Fly  4696 EPRMQ---DASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSD- 4756
             ||::   .....|.|.|:..........|.:...|.:.|         .:|..:::  |...|
Human   871 -PRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIF---------YINHNIKR--TQWEDP 923

  Fly  4757 --------GPFAVLVDHTRILDFDVKRKY--FQTELERLDEGIRREEHTVSVRRVTVFEDSFRVL 4811
                    ||   .|.::|    |.||||  |:.:|::.::...:.|  :.:||.||.|||:|.:
Human   924 RLENVAITGP---AVPYSR----DYKRKYEFFRRKLKKQNDIPNKFE--MKLRRATVLEDSYRRI 979

  Fly  4812 YRLGPEEW-KNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSH 4875
            ..:...:: |.|.:|.|:.|:|.|.||:.|||:.:||:|:|||.|.||..|..|..|..|||:|.
Human   980 MGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSG 1044

  Fly  4876 -ANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMK 4939
             .|.:|||||||:|||...||:..|||:.:|.|.|||.:|.|.:...||||.|.|:|..|.::::
Human  1045 LCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILE 1109

  Fly  4940 NDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFL 5004
            ||.:.|  :|.|..:.:.||.|...:||..|.:..||.:||.||:.||.|.:....|::|:.||.
Human  1110 NDPTEL--DLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFK 1172

  Fly  5005 EGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEY-HKYTSKSAQIQWFWRALRSFDQA 5068
            |||:::||:.||.||:|.|||||:.||.|:|:.|.:.:|:| :.|::....|||||:|:...|..
Human  1173 EGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSE 1237

  Fly  5069 DRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKL 5133
            .|.:.||||||||:||:.||..|.|.||.|.|.:.:.. :.::||.||||||:||||.|:|:::|
Human  1238 KRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWG-TPEKLPRAHTCFNRLDLPPYESFEEL 1301

  Fly  5134 RSCLLKAIHECSEGF 5148
            ...|..|| |.::||
Human  1302 WDKLQMAI-ENTQGF 1315

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075
		HECTc	4796..5149	CDD:238033	161/356 (45%)
		HECTc	4820..5148	CDD:214523	151/329 (46%)
NEDD4	NP_001271267.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	204..229		3/24 (13%)
		C2	<517..570	CDD:417471	4/52 (8%)
		Mediates interaction with TNIK. /evidence=ECO:0000250	578..981		82/458 (18%)
		WW	611..643	CDD:197736	2/31 (6%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	755..780		8/24 (33%)
		WW	769..798	CDD:395320	4/28 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	796..834		7/39 (18%)
		WW	842..871	CDD:395320	10/51 (20%)
		WW	893..925	CDD:197736	6/42 (14%)
		HECTc	986..1315	CDD:214523	151/332 (45%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.810