DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and Nedd4

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	NP_648993.1	Gene:	Nedd4 / 39958	FlyBaseID:	FBgn0259174	Length:	1007	Species:	Drosophila melanogaster

Alignment Length:	1189	Identity:	311/1189 - (26%)
Similarity:	466/1189 - (39%)	Gaps:	259/1189 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly  4026 LSAVFGMPSPTAGSGGGAGSGSAASGGLTGYDDV---PPGGTLDFRSRFCRYQVVNRLRSIFDDH 4087
            |.|...:|.|:        |.|:.:||     ||   ||      |.|........:.|..|.:.
  Fly     8 LVAAAALPVPS--------SSSSVAGG-----DVPRPPP------RRRAASVAGQQQTRQEFGNG 53

  Fly  4088 PAPKPSVPL--DEAPSTS-KAAAAKAAATAKVSTAGSQNKHSVPYQTNAKNFWDVVLNIDQ---Q 4146
            ..|:.|:..  |...|.. :.......:.||....|:    |.||.....|..:..:|||.   :
  Fly    54 YTPRRSLAAVNDSGDSCHLRIVVLTGQSLAKKDIFGA----SDPYVRIDLNTINGDINIDSVLTK 114

  Fly  4147 TQVRLANVAQFPLTSQPAWEWQPNTADFLSFSESPFGQLLEMLPYKVICRSPHLTDMLVKLLASL 4211
            |:.:..|         |.|     ..:|: |...|..   ..|.::|...:....|..:.::...
  Fly   115 TKKKTLN---------PTW-----NEEFI-FRVKPSE---HKLVFQVFDENRLTRDDFLGMVELT 161

  Fly  4212 STELPKEEELLPLSDQMPPLVP---IGPVQRAGAAAAAAHPPPPQPPAENTATATEIGDGLAANI 4273
            ...||.|:|...:.:|...|.|   :|...|........|                   ......
  Fly   162 LVNLPTEQEGRTIGEQSYTLRPRRSVGAKSRIKGTLRIYH-------------------AFIRET 207

  Fly  4274 EEQASPASGNPASGD------SNAGAKRTQ---LPTLS-VALPVTPSKPRRKIYSKNFSQLQLVI 4328
            .||:.|:||| :.|:      :|||....|   .||.. .||| ...:.|:....:.:
  Fly   208 REQSEPSSGN-SDGEWEHVEATNAGETSAQPHPFPTGGHDALP-AGWEERQDANGRTY------- 263

  Fly  4329 EVLTHQCGTTEGLDNVAKLIVNLTQCSQASSAIFIQYLTAAILGLAEEVRQAIQMLLNEIRMYNI 4393
             .:.|...||: .|....|..:.:|.:....|...|......:...|..|.|..:..|.|...|
  Fly   264 -YVNHTARTTQ-WDRPTVLNSHSSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIEDNN- 325

  Fly  4394 HQGGAGTTSQAASTSGAAAATASYVPTVSGASLLPNLAVHEGIMQDRF-TAEHVIISAPKNAKPT 4457
            :..|...|.:.|:||.|...|    || :...:|..:|:.....:|:. |.:|..........|.
  Fly   326 NAAGLAYTPKTAATSSAPPNT----PT-NNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPV 385

  Fly  4458 CELQLPSMKKLMSNSSAQPYFLRTLKIFMQVRDMYEAQNGLS--TGGGVACGAG----------- 4509
            ...|.......:.|.      ||.::....|.|: ...|..:  ..|.:|.|||
  Fly   386 AHRQPEISATSLQND------LRPVREAPGVPDI-AITNPFTRRAAGNMAGGAGWQQERRRQQMQ 443

  Fly  4510 -------------DNDDDIIDIEGDGGASGGSSSDMAPGSASTISPPPPMETNQIAQ------DG 4555
                         ..:..::|::.                    ....|....|..|      :.
  Fly   444 LHIQQHQQRQQQQQQNRILLDVDH--------------------RQQEPQHRGQRHQQQHRPSNE 488

  Fly  4556 DDDEEDDDEARSFPGVGNHAVASASKSTKPTLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQ 4620
            |.|..|...............:....:..|.:.|        ||..|     ||.......:.:.
  Fly   489 DTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQ--------NTGGE-----EEPLPPRWSMQVA 540

  Fly  4621 PTVEAFFLIHASHRGA--SKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVS 4683
            |....||:.|||.|..  ..::||                         |:|...:.....||:.
  Fly   541 PNGRTFFIDHASRRTTWIDPRNGR-------------------------ASPMPNQTRRVEDDLG 580

  Fly  4684 SANTPSSASFDDEPRMQ------DASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTV 4742
                |....:::.....      |.:|.|....:|..|....|.:|
  Fly   581 ----PLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLSNPNIAGQA------------------- 622

  Fly  4743 LNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDS 4807
                                |.::|  |:..|.:||::.:.:......:.|  :.:||.::.|||
  Fly   623 --------------------VPYSR--DYKQKYEYFKSHIRKPTNVPNKFE--IRIRRTSILEDS 663

  Fly  4808 FRVLYRLGPEE-WKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMIN 4871
            :|::..:...: .|.:.::.||.|.|.|.|||.|||:.::|:|:|||.|.||..|..|..|..||
  Fly   664 YRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQIN 728

  Fly  4872 PSSH-ANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLD 4935
            ..|. .|..|||||||:||:...||:..|||:.:|.|.|||.:|.|.:...||||.|.|:|..|.
  Fly   729 NGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLM 793

  Fly  4936 YLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQL 5000
            ::.:||...|  ||||..:...||.....:|||.|.:..||.|||.||::||.:.:....:::|:
  Fly   794 WIKENDPRIL--ELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQM 856

  Fly  5001 DAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYH-KYTSKSAQIQWFWRALRS 5064
            .:||:||..|||.:||.||:|.|||||:.|:.:||::|.:.||.|. .|......|||||||:.|
  Fly   857 SSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLS 921

  Fly  5065 FDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKS 5129
            |....|::.||||||||:||:.||..|.|.||.|.|.|.:.. :.:..|.||||||:||||.|:.
  Fly   922 FSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWG-TPNNFPRAHTCFNRLDLPPYEG 985

  Fly  5130 YDKLRSCLLKAIHECSEGF 5148
            |.:|:..|:||| |.|:||
  Fly   986 YLQLKDKLIKAI-EGSQGF 1003

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075
		HECTc	4796..5149	CDD:238033	161/356 (45%)
		HECTc	4820..5148	CDD:214523	153/329 (47%)
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	30/173 (17%)
		WW	248..277	CDD:278809	7/38 (18%)
		WW	531..560	CDD:278809	7/28 (25%)
		WW	581..613	CDD:197736	5/31 (16%)
		HECTc	650..1003	CDD:238033	160/358 (45%)
		HECTc	674..1003	CDD:214523	153/332 (46%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45446983
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR11254
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	4	3.840