DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and Trip12

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	XP_038939619.1	Gene:	Trip12 / 316575	RGDID:	1306607	Length:	2074	Species:	Rattus norvegicus

Alignment Length:	2312	Identity:	456/2312 - (19%)
Similarity:	732/2312 - (31%)	Gaps:	736/2312 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly  3176 HLIKE--LKAAHQQRKQEEAARQSTTPASGSNASATSSSTTSVVGTAAPTATSSIASGLLANAVS 3238
            ||.:|  ||:|......:...|:|.  |:||:.|.....|.|                   :.|.
  Rat   157 HLDQEQQLKSAQLPSTSKAHTRKSV--AAGSSRSQKRKRTES-------------------SCVK 200

  Fly  3239 AGSGSSSLVVPPVAASATGGTRRSSIPVLIS-----------STLGPSSASSATSATGNGATSDA 3292
            :||||.|          ||...||:.|..::           ||:..||::::||::.:...|.:
  Rat   201 SGSGSES----------TGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASAS 255

  Fly  3293 TTTTGGPTRNFSSVDSNVSSETQTTTRP-PRQRGGLRQQSQL----PFNIYA----EIDLTNEQD 3348
            :|...| .|.....|.|.:..:::.:.| ||:....::||:.    .|:..|    ::.|...:.
  Rat   256 STVPAG-ARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKL 319

  Fly  3349 SEGDRSTSTTTGTAADGAESQSQHNSSSTAAQVAVPQHQRHPPVPLLPSSGRPSRPHTRLDETEL 3413
            |....|.|.|:.....|.:::......||      .:....||..|         |..|
  Rat   320 SLPGSSKSETSKPGPSGLQAKLASLRKST------KKRSESPPAEL---------PSLR------ 363

  Fly  3414 LLLQLTDRQIRNRLQGSSGHTNSSRTSGSALINARPNRDLPACLQRHDHPAGSIFPRPSANRTPA 3478
                   |..|.:..||...| |.|.||.....|...|       |.:..|.     |..|:
  Rat   364 -------RSTRQKTTGSCAST-SRRGSGLGKRGAAEAR-------RQEKMAD-----PEGNQ--- 405

  Fly  3479 PTLVSMPTPMSNAALEQLNVTAPDVVRTDRNFALPSSLEVDVDVLPGIVDLPQQEAQPEDDQL-- 3541
                           |.:|.:|   .|||.   .|........|...:......|::.:|.::
  Rat   406 ---------------ETVNSSA---ARTDE---APQGAAASSSVAGAVGMTTSGESESDDSEMGR 449

  Fly  3542 --LLPPPPPPPPSDTTPLVHAEDDLWPGTVVSAGTREESSAPAVATESTEAVNESNQPEPTPE-- 3602
              .|......||....||......|:..|:.|..    ||......:..:|.:||.|.:...|
  Rat   450 LQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGA----SSKAQQLLQGLQASDESQQLQAVIEMC 510

  Fly  3603 -----SSESTSPNPQAPSAEP---TPLEPGAAVDASVGAQTPATTEEAASTAGASGTTATITDMS 3659
                 .:|.|.......|..|   |.|:    ::.:......|.............::|.:.|..
  Rat   511 QLLVMGNEETLGGFPVKSVVPALITLLQ----MEHNFDIMNHACRALTYMMEALPRSSAVVVDAI 571

  Fly  3660 PEVRAALGDLEVPEGVDPSFLAALPSEM-----REEVIQEH------LRMQRIRQRAQQNAIQIA 3713
            |   ..|..|:|.:.:|.:..|....||     .:.::|..      |.::.....||:||:.||
  Rat   572 P---VFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIA 633

  Fly  3714 HDSLVEVNPE---FLA-ALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNL------P 3768
            .:....:.|:   |:| :|||      |.|:...|.:::.::        |...|..|      .
  Rat   634 ANCCQSITPDEFHFVADSLPL------LTQRLTHQDKKSVES--------TCLCFARLVDNFQHE 684

  Fly  3769 ENLRQAI-----LTDMEESQIASLP---------------------PELAAEAQFLRRDWE---- 3803
            |||.|.:     ||::::..:.:.|                     |.||  .|.:::.:.
  Rat   685 ENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLA--VQLMKQSFTDIAE 747

  Fly  3804 --------SRNGA---RMDAHP--------------------PSNALSRFQTTL-----QSLDDT 3832
                    :.||:   ::|..|                    |...:....|.|     |:.|..
  Rat   748 TLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGA 812

  Fly  3833 RWHSSIWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFL 3897
            .|.   |.|..|...|.:...:.|:....|     .:|        |||      .||:||....
  Rat   813 IWQ---WRDDRGLWHPYNRIDSRIIEVAAH-----QVG--------EDE------ISLSTLGRVY 855

  Fly  3898 FVEDQKVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNEDNANFGAGQAGGKPEWLKLRVDA 3962
            .::   .|||                           |:.|||.....|.|....|    |....
  Rat   856 TID---FNSM---------------------------QQINEDTGTARAIQRKPNP----LANTN 886

  Fly  3963 AFGYKSNIFLINRLYEGSARS--ISINPQAAQMIVRNCLDLLFVLAKHYPSSFVPFNRDVKARRI 4025
            ..||..       |.:..||:  :..:|:.|:..::.   |..||.:.|.||..|..|....|.|
  Rat   887 TSGYSD-------LKKDDARAQLMKEDPELAKSFIKT---LFGVLYEVYSSSAGPAVRHKCLRAI 941

  Fly  4026 LSAVFGMPSPTAGS--GGGAGSGSAASGGLTGYDDVPPGGTLD------------FRSRFCRYQV 4076
            |..::...:.....  ...|.|...|| .|:..|.....|.|.            |...|.|..|
  Rat   942 LRIIYFADAELLKDVLKNHAVSSHIAS-MLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGV 1005

  Fly  4077 VNRLRSIFDDHPAPKPSVPLDEAPSTSKAAAAKAAATAKVSTAGSQNKHSVPYQTNAKNFWDVVL 4141
            :::::.:               |.|.|...:...|.|....:.||....|....|.|.|
  Rat  1006 MHQVKHL---------------AESESLLTSPPKACTNGSGSLGSTTPASSGTATAATN------ 1049

  Fly  4142 NIDQQTQVRLANVAQFPLTSQPAWEWQPNTADFLSFSESPFGQLLEMLPYKVICRSPHLTDMLVK 4206
                         |...|.|......:.::.|.     ||.|:|.::|..|
  Rat  1050 -------------ASADLGSPSLQHSRDDSLDL-----SPQGRLSDVLKRK-------------- 1082

  Fly  4207 LLASLSTELPKEEELLPLSDQMPPLVPIGPVQRAGAAAAAAHPPPPQPPAENTATATEIGDGLAA 4271
                   .|||.                      |.......||......:|.|.:........:
  Rat  1083 -------RLPKR----------------------GPRRPKYSPPRDDDKVDNQAKSPTTTQSPKS 1118

  Fly  4272 NIEEQASPASGNPASGDSNAG----AKRTQLPTLS-VALPVTPSKPRRKI--------------- 4316
            :.....:|.:....|..||:.    |:...:..|: .|...|.|..|.||
  Rat  1119 SFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERY 1183

  Fly  4317 -YSKNFSQLQLVIEVLTHQCGTTEGL----DNVAKLIVNLTQCSQAS--SAIFIQ---------- 4364
             .|:|.......:.||...|..||.|    |..|:.:|.:......|  |:..||
  Rat  1184 FSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLL 1248

  Fly  4365 YLT----------------------------------------AAILGLAEEVRQAI-QMLLNEI 4388
            |||                                        |.:|.|..::...: ||....:
  Rat  1249 YLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPV 1313

  Fly  4389 RMYNIHQGGAGTTSQAASTSGAAAATASYVPTVSGASLLPNLAVHEGIMQDRFTAEHVIISAPKN 4453
            ::::...|..                       ||.|    .:::.|....:|...|.:
  Rat  1314 KVHDFPSGNG-----------------------SGGS----FSLNRGSQALKFFNTHQL------ 1345

  Fly  4454 AKPTCELQLPSMKKLMSNSSAQPYFLRTLKIFMQVRDMYEAQNGLSTGGGVACGAGDNDDDIIDI 4518
               .|:||.......:......|..:..|.: :|..:.|....|:...|.|.....|:|||..|.
  Rat  1346 ---KCQLQRHPDCANVKQWKGGPVKIDPLAL-VQAIERYLVVRGIFRYGRVREDDEDSDDDGSDE 1406

  Fly  4519 EGDGGA------SGG--------SSSDMAPGSASTISPPPPMETNQIAQDGDDDEE--DD----- 4562
            |.|...      ||.        ....:.|.:.:..     ....|.:...:|:.|  ||
  Rat  1407 EIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVY-----QAVRQFSVQAEDERESTDDESNPL 1466

  Fly  4563 -------------------DEARSFPGVGNHAVASASKSTKPTLSQILCLDVLW---------NT 4599
                               ||..|...||.....:.:..||.:.......|.||         |.
  Rat  1467 GRAGIWTKTHTIWYKPVREDEESSKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANP 1531

  Fly  4600 LSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGG 4664
            |...|:........|.    .|:::...|:...|  |..:.......||
  Rat  1532 LEVYLIPTPPENITFE----DPSLDVILLLRVLH--AISRYWYYLYDNA---------------- 1574

  Fly  4665 LGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQD----ASTNTPLVTNPSTSTAMSAHEAQL 4725
                  ..:|..|..:.::|..|..:     ..::||    .:.|.|........|.........
  Rat  1575 ------MCKEIIPTSEFINSKLTAKA-----NRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDT 1628

  Fly  4726 RQ--------DRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTEL 4782
            ||        ||.:.:|                                |:||.:.:.....::.
  Rat  1629 RQMLFYVTAFDRDRAMQ--------------------------------RLLDTNPEINQSDSQD 1661

  Fly  4783 ERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIIS 4847
            .|:...:.|::.||:  |..:.:.:..|:..||..  :....|.:|:|.|...|..| |:|.::|
  Rat  1662 SRVAPRLDRKKRTVN--REELLKQAESVMQDLGSS--RAMLEIQYENEVGTGLGPTL-EFYALVS 1721

  Fly  4848 REIFNPMYALFCVSPGDRVTYMINPSSH---------------------ANPNHLS----YFKFV 4887
            :|:......|:   .|:.|| :.||...                     |.|.|::    .|:|:
  Rat  1722 QELQRADLGLW---RGEEVT-LSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFL 1782

  Fly  4888 GRVIAKAVHDNKLLECYFTRSFYKHIL------------------GKQVKHTD--------MESQ 4926
            |:::|||:.|.:|::......|||.:|                  .:.|.|.:        :|..
  Rat  1783 GKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQD 1847

  Fly  4927 DYEFYKGLDYLMKN------DISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQ 4985
            ..:..:.|.|.::.      .:..||.:.|..      |...| :||..|:|..||..|..||::
  Rat  1848 KSQTKESLQYALETLTMNGCSVEDLGLDFTLP------GFPNI-ELKKGGKDIPVTIHNLEEYLR 1905

  Fly  4986 LVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEY----H 5046
            ||....::..:.:|.|:|.:||..:.|...:..|..:||:.|:.| ...|..|.|...|.    |
  Rat  1906 LVIFWALNEGVCRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCG-SKADTWDAKTLMECCRPDH 1969

  Fly  5047 KYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDR 5111
            .||..|..:::.:..|.|||...:..|||||||:.::|:.||.||.....|.:......:...|.
  Rat  1970 GYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDF 2034

  Fly  5112 LPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGF 5148
            ||...||.|.|.||.|.|.:.:|..||.|..|..:.|
  Rat  2035 LPSVMTCVNYLKLPDYSSIEIMRDKLLIAAREGQQSF 2071

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740	14/85 (16%)
		DUF4414	3675..3779	CDD:291075	30/129 (23%)
		HECTc	4796..5149	CDD:238033	110/414 (27%)
		HECTc	4820..5148	CDD:214523	104/388 (27%)
Trip12	XP_038939619.1	SRP1	489..>714	CDD:227396	49/245 (20%)
		WWE	814..876	CDD:397111	23/113 (20%)
		HECTc	1672..2072	CDD:238033	111/417 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.900