Protein Alignment HUWE1 and Itch

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: NP_001005887.2
• Gene: Itch / 311567
• RGDID: 1359556
• Length: 864
• Species: Rattus norvegicus
• Alignment Length: 940
• Identity: 259/940 - (27%)
• Similarity: 413/940 - (43%)
• Gaps: 182/940 - (19%)

  Fly  4300 TLSVALPVTPSKPRRKIYSKNFSQLQLVIEVLTHQCGTTEGLDNVAKLIVNLTQCSQASSAIFIQ 4364
            |:...|.:|....:.|...||:......:||      |.:|.....:      :|:..:|..:.|
  Rat    15 TMKSQLQITVISAKLKENKKNWFGPSPYVEV------TVDGQSKKTE------KCNNTNSPKWKQ 67

  Fly  4365 YLTAAILGLAEEVRQAIQMLLNEIRMYNIHQGGAGTTSQAASTSGAAAATASYVPTVSGASLLPN 4429
            .||..:..:::..          .|::: ||     |.::....|.|                 .
  Rat    68 PLTVIVTPMSKLC----------FRVWS-HQ-----TLKSDVLLGTA-----------------G 99

  Fly  4430 LAVHEGIMQDRFTAEHVIISAPKNAKPTCELQLPSMKKLMSNSSAQPYFLRTLKIFMQVRDMYEA 4494
            |.::|.:..:....|.|:::          |||...|:...........|..|::        ||
  Rat   100 LDIYETLKSNNMKLEEVVMT----------LQLVGDKEPTETMGDLSVCLDGLQV--------EA 146

  Fly  4495 QNGLSTGGGVAC--GAGDND-----------------DDIIDIEGDG-GASGGSSSDMAPGS--- 4536
            :  :.|.|..:|  ||..||                 |..:...|:. .|:|.:|..::.|.
  Rat   147 E--VVTNGETSCSEGATQNDAGCRPKDETRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKP 209

  Fly  4537 ---ASTISPPPPMETNQIAQDGD---DDEEDDDEARSFPGVGNHAVASASKST------------ 4583
               .....||||......:.:|.   :.:.|.....|.|....:..:|||:..
  Rat   210 SRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNSSASEGAASGLIIPLTISG 274

  Fly  4584 ----KP--TLSQ----------------ILCLD-----VLWNTLSECLVALEESKDEFAVLVL-- 4619
                :|  |:||                :..:|     ..|:.........|...|....:..
  Rat   275 GSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVD 339

  Fly  4620 ---------QPTVEA------FFLIHASHRGASKKSGRSFMPNAAGFG---LRAAAGAGAIGGLG 4666
                     :||:|:      :.|..:..:||.::..:.|:     ||   |.|.:.......||
  Rat   340 HFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFI-----FGNQDLFATSQNKEFDPLG 399

  Fly  4667 AAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQK 4731
            ...|..::.:.....|...|..:..:..::||.|......||         ....|.:...|...
  Rat   400 PLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPL---------PEGWEMRFTVDGIP 455

  Fly  4732 FLQFAEKHRTVLNQI-LRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEH- 4794
            :  |.:.:|.....| .|...:.|.:||....|     .||..|.:||:...::|    ...:|
  Rat   456 Y--FVDHNRRATTYIDPRTGKSALDNGPQIAYV-----RDFKAKVQYFRFWCQQL----AMPQHI 509

  Fly  4795 TVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFC 4859
            .::|.|.|:|||||:.:....|::.:.|.:::|..|||.|.||:.|||:.::|.|:.||||.||.
  Rat   510 KITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFE 574

  Fly  4860 VSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDME 4924
            .:..|.....|||:|:.||:||.||:|:||.||.|:...|.::..|:..|||.||.|.|...|:|
  Rat   575 YAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLE 639

  Fly  4925 SQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQ 4989
            |.|.|||..|.::.:|:|...|.|:.||.:.:..|..:..||||||.:..||||||.||:::|.:
  Rat   640 SIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAE 704

  Fly  4990 LKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQ 5054
            .::|..:.:|..||.|||.:|:|:..:..|:.:|||:|:.|:.:||:.|.:.:..|..||..|.|
  Rat   705 WRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQ 769

  Fly  5055 IQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCF 5119
            |.|||:.::..|...|.:.|||||||.::|:.||..|.|.||.|||.|.:..:. :.||.:||||
  Rat   770 IVWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKE-NWLPRSHTCF 833

  Fly  5120 NQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5149
            |:||||.||||::|:..||.||.| :||||
  Rat   834 NRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 862

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159
		HECTc	4796..5149	CDD:238033	154/352 (44%)
Itch	NP_001005887.2	C2_E3_ubiquitin_ligase	17..140	CDD:175988	28/177 (16%)
		WW	289..318	CDD:459800	2/28 (7%)
		HUL4	<320..864	CDD:227354	196/570 (34%)