DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and Magi3

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	NP_620784.3	Gene:	Magi3 / 245903	RGDID:	621362	Length:	1470	Species:	Rattus norvegicus

Alignment Length:	1712	Identity:	297/1712 - (17%)
Similarity:	486/1712 - (28%)	Gaps:	672/1712 - (39%)

- Green bases have known domain annotations that are detailed below.

  Fly  1609 NHLNKQVFVTN-FVRNIIQWVDFTLQLFEPAKTTGSKASRLEECLTGMTATRLFVRLKTSTLLLE 1672
            :|..:||...| ::|.|            |..|...:...:........:...|..|:.|..|||
  Rat   129 DHKLQQVIRDNLYLRTI------------PCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLE 181

  Fly  1673 E-----NYSDLHRPLVEAITAQDAMVSLVKLLDCMSQWMLEQSTETNTGQQRATVPRWVQNLVDL 1732
            .     |:....:|..|....|.         |.:.|.:.:...:|.:.::|.|....::.:...
  Rat   182 SGTYDGNFYGTPKPPAEPSPFQP---------DPVDQVLFDNEFDTESQRKRTTSVSKMERMDSS 237

  Fly  1733 I---------DAIDSISHILQRKANMRAVSSDVWRWYDASTGKWNAYSEANNELIR--NAYAAGE 1786
            :         :|::....:..|:  |.:.|||.|.....|..:.|...:..|.::|  |.....:
  Rat   238 LPEEEEDEDKEAVNGSGSMETRE--MHSESSDCWMKTVPSYNQTNRSMDFRNYMMRDENLEPLPK 300

  Fly  1787 RW--LYINIG-------RQRCTVSLNCMTQVSEASGTHRP-VCPALKLSEAITSLNNPVALHKVE 1841
            .|  .|.:.|       ..:.|..|:             | :|...|..|.......|....|:|
  Rat   301 NWEMAYTDTGTIYFIDHNTKTTTWLD-------------PRLCKKAKAPEDCEDGELPYGWEKIE 352

  Fly  1842 HILNRVVRTAPDGSGTVHSDELISLRQLVNFTDGQQPQQSENSANPSEEATSEDGPAAANSGSPP 1906
            .         |. .||.:.|.|            .|..|.|   ||.||
  Rat   353 D---------PQ-YGTYYVDHL------------NQKTQFE---NPVEE---------------- 376

  Fly  1907 ATMTTRSKTRQKSAAAAAAAKNKSQSGGSPPIPKRTQKIILNEEQVGLSKFDCGRIIGSIVDLLQ 1971
                               ||.|.|.|               :.:...:|.|..|
  Rat   377 -------------------AKRKKQIG---------------QAETHSAKTDVER---------- 397

  Fly  1972 PSHQMLNETKLSLLRLCARLTRNYDNAQVFIRRGGVRMLLHLQQACSFMGFPTYAN------IIL 2030
                             |..||  |.:|:    .||.:...|:::....||.....      :.:
  Rat   398 -----------------AHFTR--DPSQL----KGVLVRASLKKSTMGFGFTIIGGDRPDEFLQV 439

  Fly  2031 RHVLEAPPVLQEAMERVLGMRAAGIVPVG----------HRDLI--YVLTQVSSAV--------- 2074
            ::||:..|..|:      |..|.|.|.|.          |.|::  :.|..|:..|
  Rat   440 KNVLKDGPAAQD------GKMAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYA 498

  Fly  2075 ----SRNPQIFVAAAKEMLKGEYLINTNSTTLADDARV--MVKSKFG------------------ 2115
                |.:|.:.:.||..::.|:.|....:.....|.::  ||..:.|
  Rat   499 LPDDSEDPVVDIVAATPVINGQSLAKGEACMSTQDFKLGAMVLDQNGKSGKLLSSDHLNGPSDSN 563

  Fly  2116 -QQPTAAAGGATPHNQTPTP----PPAAKDELKDDPQVQSSVAVL--------------KELLHG 2161
             |:.:.|:.|::.......|    |......:.|.|..|....:|              ||:.|.
  Rat   564 EQRASLASSGSSQPELVTIPLVKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQ 628

  Fly  2162 LVQPCWHY---------------------GG-----------------TGSLDR----------- 2177
            .||...|.                     ||                 :|||:.
  Rat   629 NVQNLTHLQVVEVLKQFPVGADVPLLILRGGPCSPTKTAKMKTDTKETSGSLETINEPTPQPMPF 693

  Fly  2178 -----GQGAAEL-----------LQPDQPPGTAQGCHDDELYFRFQETPAPVSSKKQSSSGGGAA 2226
                 ..|:.:|           |..|:||.|    .|.:::.|.||            ||.|..
  Rat   694 PPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNT----KDLDVFLRKQE------------SGFGFR 742

  Fly  2227 AAGQTGQ-------------ATEK------FEPCCCTWHIPTSGHTHAKPTISRATLLKLLADST 2272
            ..|..|.             |.||      .:...|...||..|.:|.:       :|.|:..:.
  Rat   743 VLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQ-------VLDLMTTAA 800

  Fly  2273 R------------AYQSLVPRVLLSHVYTPADSPLI--------SAPQQTFLGVLLDR------- 2310
            |            .|....|.......::.:.||.:        ||||::: .|:|.|
  Rat   801 RNGHVLLTVRRKIFYGEKQPEDESPQAFSQSGSPRLNRTELPTRSAPQESY-DVILQRKENEGFG 864

  Fly  2311 FLPITQRHHVPEVSTMTRVLICSLSDCYQQPGVQVHVAEELHAAVARALAQPDSAEKHTRLQVLM 2375
            |:.:|.:...|                   |||..|       .:.|.: ....|::..||:|..
  Rat   865 FVILTSKSKPP-------------------PGVIPH-------KIGRVI-DGSPADRCGRLKVGD 902

  Fly  2376 SLFPNLIESPMLYDKVHMHRNNMYRVLLRQGVMTYLTKVAQYKDLSNHNTLSTLAAILRPMEILL 2440
            .:  :.:....:.|..|   :|:.:::...||...||.||:    ..|:
  Rat   903 HI--SAVNGQSIVDLSH---DNIVQLIKDAGVTVTLTVVAE----EEHH---------------- 942

  Fly  2441 RFSVSTTTLGSKRILFGSGGPGGPNASGNGNNTAGGSSGNGPGSGNTYGTIINRRLYPRLAARTG 2505
                              |.|.|.|::                           |..|.|..|.
  Rat   943 ------------------GPPSGTNSA---------------------------RQSPALQHRP- 961

  Fly  2506 FGSAMERSNAMGGEHVLR-DIIRNVLSDRRHAFEFIFNSDDMAVDSEDAAAPGSGVGGGGSGAGS 2569
            .|.| :.::..|....|. ::.::|.|..||::.          |.:..|.|
  Rat   962 MGQA-QATHIPGDRTALEGEVGKDVCSSYRHSWS----------DHKHLAQP------------- 1002

  Fly  2570 VGGGVSGSVSGNSSAAVIDVVEERSNGGDTNDPPANVSSASSGAGQSSTSGGPVRPVVNFDQLWD 2634
                         ..|||.||..| :.......|..:.....|.|.|...|......:
  Rat  1003 -------------DTAVISVVGSR-HSQSLGCYPVELERGPRGFGFSLRGGKEYNMGL------- 1046

  Fly  2635 DFLQSEGLRLASRSGGGAGSGSGSVG---ASSSGQ--QNGSNTTA-SSTNSGNNSGIGNLRLRTN 2693
             |:    |||| ..|.....|...||   ...:|:  |..::|.| ....:|.|..:..||..|.
  Rat  1047 -FI----LRLA-EDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTG 1105

  Fly  2694 PDVDVGSGNGSGSGSGSNAGVVNDNGISGGGGSDGASTSSDTGAP-------------------G 2739
            ...|.|..:   ..|.|::.|:.|........|..|:|..::..|                   .
  Rat  1106 LIPDHGDWD---IYSPSSSNVIYDEQPPPLPSSHSAATFEESHVPVTEDSLIRVQTCEKAEELKD 1167

  Fly  2740 GARDLNASLLG--------DVSTSESDSDPSTEN---DERNEEDED-----EDDDAPDEDVDEHS 2788
            ..::..::|.|        .|..:.|..|||..:   |:...:.|:     ....:|.:.|:.||
  Rat  1168 TVQEKKSTLNGSQPEMKYQSVQKNVSKKDPSRSHGHGDKNLPKGENGVTRRGRSASPKKSVNRHS 1232

  Fly  2789 ETDVDEERSRQFIEVFDHIYEPESSETASNDADVDNDDDDDEDDVDDDDDDDEDNDDDVREEVQS 2853
            |            |..:.|..|..|:......|                              :|
  Rat  1233 E------------EHLEKIPRPLRSDPKGKSRD------------------------------RS 1255

  Fly  2854 VMDAEIENRIDIALALVSS-----NNRPRRSAAVNASGDGSANRTNPDDEVDD---------EDD 2904
            :...:.||:..:.:...|.     .:|.|.|:.......|..:.:|.:.::.:         ..|
  Rat  1256 LSPRKGENKGQVTIKAGSGQDPCRKDRGRSSSPRKQQKIGGNSLSNTEGKLSEAGSRRAAGLSSD 1320

  Fly  2905 DVDADPDG----------------------DPD-----VDPISGPS--AAQAEAYLYERLGSGLM 2940
            ..:..|:|                      .|:     ||..|.||  .:...:.:......|..
  Rat  1321 SPEQLPEGKEKSGVSRKDLKLSQLGKNRTRSPEKRSSKVDEASLPSKKTSDTASRVVSEKEKGRK 1385

  Fly  2941 PTPPVGFRVRMNSSI---AQPLDIDVNGGNAGSGSNAGATGSSGGSGQRSSTGGTTASIGAIASA 3002
            |......|.:...|:   |:||..:  .|...:.|.|......|    :...||...|...:..|
  Rat  1386 PGTGERSRDKTGESVQTSAKPLTQE--AGEKMALSKASEVTDRG----KERAGGAPESSSPVKKA 1444

  Fly  3003 ALRPSSGSWMAPDFNFIRISGG 3024
            .:.|  |.|..|..|.:..:.|
  Rat  1445 PITP--GPWRVPRANKVTGTAG 1464

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922	15/83 (18%)
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075
		HECTc	4796..5149	CDD:238033
		HECTc	4820..5148	CDD:214523
Magi3	NP_620784.3	Interaction with ADRB1 and TGFA. /evidence=ECO:0000269\|PubMed:16316992	18..108
		PDZ_signaling	24..103	CDD:238492
		NK	125..>199	CDD:418433	16/81 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	184..266		12/92 (13%)
		HUL4	<211..>373	CDD:227354	35/201 (17%)
		WW	299..329	CDD:238122	6/42 (14%)
		WW	345..375	CDD:238122	13/54 (24%)
		Interaction with PTEN. /evidence=ECO:0000250	413..495		17/87 (20%)
		PDZ	416..496	CDD:214570	17/85 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	550..575		3/24 (13%)
		PDZ	578..659	CDD:214570	12/80 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	665..700		3/34 (9%)
		PDZ	729..812	CDD:214570	19/101 (19%)
		Interaction with ADGRB1. /evidence=ECO:0000250	729..811		19/100 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	818..847		4/28 (14%)
		PDZ_signaling	851..936	CDD:238492	22/117 (19%)
		Interaction with LPAR2 and GRIN2B. /evidence=ECO:0000250	852..939		24/122 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..976		12/99 (12%)
		PDZ_signaling	1023..1101	CDD:238492	19/90 (21%)
		PTZ00112	1115..>1405	CDD:240274	49/331 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1124..1146		4/21 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1167..1470		58/348 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900