Protein Alignment HUWE1 and HECW1

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: XP_016867371.1
• Gene: HECW1 / 23072
• HGNCID: 22195
• Length: 1639
• Species: Homo sapiens
• Alignment Length: 2230
• Identity: 444/2230 - (19%)
• Similarity: 707/2230 - (31%)
• Gaps: 853/2230 - (38%)

  Fly  3093 GANGGTTGQHPNAT----LCTNGVRRRLLYLDQQYCVCMPTHQNPTEETQMETFTQD-ALHWWLE 3152
            |.:.|.|  .|.:|    |.|:..|..|:.....|.:               ..:|| .:||
Human    88 GPHDGVT--IPRSTSDTDLVTSDSRSTLMVSSSYYSI---------------GHSQDLVIHW--- 132

  Fly  3153 EATLLDMESQTDACLYAAHFLLPHLIKE---------LKAAHQQR--KQEEAARQSTTP------ 3200
                 |::.:.||..:...:|:..::.|         :..:|:.:  .:.:|:.....|
Human   133 -----DIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICF 192

  Fly  3201 -----ASGSNASATSSSTTSVVGTAAPTATSSIASGLLANAVSAGSGSSSLVVPPVAASATGGTR 3260
                 .||:..:.|.|.|  |..:|||     |...:.|:....|.||..|:...::.....|.:
Human   193 KYYHGVSGALRATTPSVT--VKNSAAP-----IFKSIGADETVQGQGSRRLISFSLSDFQAMGLK 250

  Fly  3261 RSSI----PVL-ISSTLGPSSASSATSATGNGATSDATTTTGGP-----TRNFSSVDSNVSSETQ 3315
            :...    |.| ||...|..|...|....|....|.....|..|     ..:|.|:.::| .|.:
Human   251 KGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDV-LEIE 314

  Fly  3316 TTTRPPRQRGGLRQ---QSQLPFNIYAEIDLTNEQDSEGDRSTSTTTG--TAADGAESQSQHNSS 3375
            ...:..:.|..:::   :..:|      :....|:.:.|||..|.|.|  ...|....|.|....
Human   315 VKDKFAKSRPIIKRFLGKLSMP------VQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFE 373

  Fly  3376 STAAQVAVPQHQRHPPVPLLPSSGRPSRPHTRLDETELLL------LQLTDRQIRNRLQGSSGHT 3434
            .|::        .||                 .|:.|:.|      .|:.|..:.|.::..||..
Human   374 ITSS--------IHP-----------------ADDEEISLSTEPESAQIQDSPMNNLMESGSGEP 413

  Fly  3435 NSSRTSGS--------------------ALINARPNRDLPACLQRHDH------PAGS------- 3466
            .|.....|                    ::..:||..:.....:..|.      |.|:
Human   414 RSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQV 478

  Fly  3467 ---IFPRPSA--------------------NRTPAPT----LVSMPTPMSNAALEQ--------- 3495
               |.|.|||                    ...||.|    |....|..|.|..|:
Human   479 QKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEG 543

  Fly  3496 -----------LNVTAPDVVRTDRNFALPSSLEVDVDVLPGIVDLPQQEAQPEDDQLLLPPPPPP 3549
                       |.:.| .|.|..|..:||.| |::..:.....|                   |.
Human   544 DVSTLEQGEGRLQLRA-SVKRKSRPCSLPVS-ELETVIASACGD-------------------PE 587

  Fly  3550 PPSDTTPLVHAEDDLWPGTVVSAGTREESSAPAVATESTEAVNESNQPEPTPESSESTSPNPQA- 3613
            .|......:|......|.....:...||..|     |....:.:|::.:...| .::.:.:|.|
Human   588 TPRTHYIRIHTLLHSMPSAQGGSAAEEEDGA-----EEESTLKDSSEKDGLSE-VDTVAADPSAL 646

  Fly  3614 --PSAEPTPLEPGAAVDASVGAQTPATTEEAASTAGASGTTATITDMSPEVRAALGDLEVPEGVD 3676
              ...||....||.|.....|...|:....||.......:|.:.:|.||...   ||... ||.|
Human   647 EEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQG---GDHSC-EGCD 707

  Fly  3677 -----PSFLAA------------------LPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLV 3718
                 ||..::                  .||.........|.|...:      ::.:|:
Human   708 ASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSV------DSAKIS----- 761

  Fly  3719 EVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDMEESQ 3783
                          :|.|...|..|::..:|              |:::|::::.
Human   762 --------------ESTVFSSQDDEEEENSA--------------FESVPDSMQS---------- 788

  Fly  3784 IASLPPELAAEAQFLRRDWESRNGA---RMDAHPPSNALSRFQTTLQSLDDTRWHSSIWYDASG- 3844
                 |||         |.||.|||   :.:...||..:.|....|:|            ..:|
Human   789 -----PEL---------DPESTNGAGPWQDELAAPSGHVERSPEGLES------------PVAGP 827

  Fly  3845 ------------NAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFL 3897
                        |:||.    :.:....|..||.|.:.:||             |.:.
Human   828 SNRREGECPILHNSQPV----SQLPSLRPEHHHYPTIDEPL-------------PPNW------- 868

  Fly  3898 FVEDQKVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNEDNANFGAGQAGGKPEWLKLRVDA 3962
               :.:::|   |..:..:.|...|..|         |:..          |...|:.::
Human   869 ---EARIDS---HGRVFYVDHVNRTTTW---------QRPT----------AAATPDGMR----- 903

  Fly  3963 AFGYKSNIFLINRLYEGSARSISINPQAAQMIVRNCLDLLFVLAKHYPSSFVPFNRDVKARRILS 4027
            ..|....:..:||.|:...|:|:..........::|
Human   904 RSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSC----------------------------- 939

  Fly  4028 AVFGMPSPTAGSGGGAGSGSAASGGLTGYDDVPPGGTLDFRSRFCRYQVVNRLRSIFDDHPAPKP 4092
                 ....||.|||.||.|.|....:..|....|......|:    ::...|:|         |
Human   940 -----EQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQ----KITLLLQS---------P 986

  Fly  4093 SVPLDEAPSTSKAAAAKAAATAKVSTAGSQNKHSV-PYQTNAKNFWDVVLNIDQQTQVRLANVAQ 4156
            :|.....|.......|..:| .:|.|:.:..||.: ..:.:|:||
Human   987 AVKFITNPEFFTVLHANYSA-YRVFTSSTCLKHMILKVRRDARNF-------------------- 1030

  Fly  4157 FPLTSQPAWEWQPNTADFLSFSESPFGQLLEMLPYKVICRSPHLTDMLVKLLASLSTELPKEEEL 4221
                     |...:..|.::|                           :.:.|....|||:..|:
Human  1031 ---------ERYQHNRDLVNF---------------------------INMFADTRLELPRGWEI 1059

  Fly  4222 LPLSDQMPPLVPIGPVQRAGAAAAAAHPPPPQPPAENTATATEIGDGLAANIEEQASPASGNPAS 4286
              .:||.            |.:....|         |:...|.|                 :|
Human  1060 --KTDQQ------------GKSFFVDH---------NSRATTFI-----------------DP-- 1082

  Fly  4287 GDSNAGAKRTQLPTLSVALPVTPSKPRRKIYSKNFSQLQLVIEVLTHQCGTTEGLDNVAKLIVNL 4351
                      ::|..:..||                      ..|||
Human  1083 ----------RIPLQNGRLP----------------------NHLTH------------------ 1097

  Fly  4352 TQCSQASSAIFIQYLTAAILGLAEEVRQAIQMLLNEIRMYNIHQGGAGTTSQAASTSGAAAATAS 4416
                                      ||.:|.|    |.|                   :|..||
Human  1098 --------------------------RQHLQRL----RSY-------------------SAGEAS 1113

  Fly  4417 YVPTVSGASLLPNLAVHEGIMQDRFTAEHVIISAPKNAKPTCELQLPSMKKLMSNSSAQPYFLRT 4481
            .|....|||||..             ..|.:::|.::......|.|....|:::       |||.
Human  1114 EVSRNRGASLLAR-------------PGHSLVAAIRSQHQHESLPLAYNDKIVA-------FLRQ 1158

  Fly  4482 LKIFMQVRDMYEAQNGLSTGGGVACGAGDNDDDIIDIEGDGGASGGSSSDMAPGSASTISPPPPM 4546
            ..||..:::.                                                   .|.:
Human  1159 PNIFEMLQER---------------------------------------------------QPSL 1172

  Fly  4547 ETNQIAQDGDDDEEDDDEARSFPGVGNHAVASASKSTKPTLSQILCLDVLWNTLSECLVALEESK 4611
            ..|...:         ::.......|||.           |.::.|
Human  1173 ARNHTLR---------EKIHYIRTEGNHG-----------LEKLSC------------------- 1198

  Fly  4612 DEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYS 4676
            |...|::|....|...               |::|      |:||...|              ||
Human  1199 DADLVILLSLFEEEIM---------------SYVP------LQAAFHPG--------------YS 1228

  Fly  4677 PFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRT 4741
             |....|..::|.::     |.:|.||...|
Human  1229 -FSPRCSPCSSPQNS-----PGLQRASARAP---------------------------------- 1253

  Fly  4742 VLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFED 4806
                    ||             :.|  ||:.|.:.|..:||....|....:..:.:||..:.|.
Human  1254 --------SP-------------YRR--DFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEG 1295

  Fly  4807 SF-RVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMI 4870
            :| :|:.....|..:|:.|:.|..|||.|..|..||::.::|:|:|||.|.||..|..|..|..|
Human  1296 TFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQI 1360

  Fly  4871 NPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLD 4935
            :|.|....|||.:|:|.||::..|:....||:.:|||.|||.:|......:|:|..|.||::.|.
Human  1361 SPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQ 1425

  Fly  4936 YLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQL 5000
            ::..|:|:.: .:|||:...:.||....|:||..|.:|.|||:||.||::.:.:.::...:.||.
Human  1426 WMKDNNITDI-LDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQT 1489

  Fly  5001 DAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYH-KYTSKSAQIQWFWRALRS 5064
            :|.:.|||:::...|:|:|:.:||||:|:|..:||:.|.:.||||. .|......|:|||.|:..
Human  1490 EALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVER 1554

  Fly  5065 FDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKS 5129
            |:...|.:.||||||||.||.:||.:|.|.||:::|.|.:..:.|. ||.||||||:||||.|.|
Human  1555 FNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITS-LPRAHTCFNRLDLPPYPS 1618

  Fly  5130 YDKLRSCLLKAIHECSEGFG 5149
            |..|...||.|:.|.|. ||
Human  1619 YSMLYEKLLTAVEETST-FG 1637

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521	87/482 (18%)
		UBM	3670..3700	CDD:464159	9/52 (17%)
		UBM	3719..3746	CDD:464159	4/26 (15%)
		HECTc	4796..5149	CDD:238033	142/354 (40%)
HECW1	XP_016867371.1	HECW_N	97..214	CDD:465177	24/141 (17%)
		C2_NEDL1-like	237..373	CDD:176073	28/142 (20%)
		PHA03169	<407..>531	CDD:223003	20/123 (16%)
		WW	864..893	CDD:459800	8/63 (13%)
		HECW1_helix	981..1047	CDD:465766	17/131 (13%)
		WW	1053..1084	CDD:197736	11/82 (13%)
		HECTc	1283..1637	CDD:238033	142/356 (40%)