DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment HUWE1 and Itch

DIOPT Version :9

Sequence 1:	NP_001285288.1	Gene:	HUWE1 / 32510	FlyBaseID:	FBgn0030674	Length:	5151	Species:	Drosophila melanogaster
Sequence 2:	NP_001230641.1	Gene:	Itch / 16396	MGIID:	1202301	Length:	864	Species:	Mus musculus

Alignment Length:	955	Identity:	257/955 - (26%)
Similarity:	418/955 - (43%)	Gaps:	188/955 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly  4289 SNAGAKRTQLPTLSV--ALPVTPSKPRRKIYSKNFSQLQLVIEVLTHQCGTTEGLDNVAKLIVNL 4351
            |::|.:...:.:|::  .|.:|....:.|...||:......:||      |.:|.....:
Mouse     2 SDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEV------TVDGQSKKTE----- 55

  Fly  4352 TQCSQASSAIFIQYLTAAILGLAEEVRQAIQMLLNEIRMYNIHQGGAGTTSQAASTSGAAAATAS 4416
             :|:..:|..:.|.||..:...::..          .|::: ||     |.::....|.|
Mouse    56 -KCNNTNSPKWKQPLTVIVTPTSKLC----------FRVWS-HQ-----TLKSDVLLGTA----- 98

  Fly  4417 YVPTVSGASLLPNLAVHEGIMQDRFTAEHVIISAPKNAKPTCELQLPSMKKLMSNSSAQPYFLRT 4481
                        .|.::|.:..:....|.|:::          |||...|:...........|..
Mouse    99 ------------GLDIYETLKSNNMKLEEVVMT----------LQLVGDKEPTETMGDLSVCLDG 141

  Fly  4482 LKIFMQVRDMYEAQNGLSTGGGVAC---------GAGDNDDDIIDIEG-----------DGGASG 4526
            |::        ||:  :.|.|..:|         |....||..:...|           :..|:|
Mouse   142 LQV--------EAE--VVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANG 196

  Fly  4527 GSSSDMAPGS------ASTISPPPPMETNQIAQDGD---DDEEDDDEARSFPGVGNHAVASASKS 4582
            .:|..::.|.      .....||||......:.:|.   :.:.|.....|.|....:...|.|:.
Mouse   197 NNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEG 261

  Fly  4583 TKPTLSQILCLDVL-------WNTLSECLV--ALEESKDEFAVLVL------------------- 4619
            .  |...|:.|.:.       .||:|:..:  ..|:..|:...:..
Mouse   262 A--TSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPG 324

  Fly  4620 ------------------------QPTVEA------FFLIHASHRGASKKSGRSFMPNAAGFG-- 4652
                                    :||:|:      :.|..:..:||.::..:.|:     :|
Mouse   325 WERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFI-----YGNQ 384

  Fly  4653 -LRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTST 4716
             |.|.:.......||...|..::.:.....|...|..:..:..::||.|......||
Mouse   385 DLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPL-------- 441

  Fly  4717 AMSAHEAQLRQDRQKFLQFAEKHRTVLNQI-LRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQT 4780
             ....|.:...|...:  |.:.:|.....| .|...:.|.:||....|     .||..|.:||:.
Mouse   442 -PEGWEMRFTVDGIPY--FVDHNRRATTYIDPRTGKSALDNGPQIAYV-----RDFKAKVQYFRF 498

  Fly  4781 ELERLDEGIRREEH-TVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYV 4844
            ..::|    ...:| .::|.|.|:|||||:.:....|::.:.|.:::|..|||.|.||:.|||:.
Mouse   499 WCQQL----AMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFF 559

  Fly  4845 IISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSF 4909
            ::|.|:.||||.||..:..|.....|||:|:.||:||.||:|:||.||.|:...|.::..|:..|
Mouse   560 LLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPF 624

  Fly  4910 YKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTA 4974
            ||.||.|.|...|:||.|.|||..|.::.:|:|...|.|:.||.:.:..|..:..||||||.:..
Mouse   625 YKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNIL 689

  Fly  4975 VTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDL 5039
            ||||||.||:::|.:.::|..:.:|..||.|||.:|:|:..:..|:.:|||:|:.|:.:||:.|.
Mouse   690 VTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDW 754

  Fly  5040 KANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHR 5104
            :.:..|..||..|.||.|||:.::..|...|.:.|||||||.::|:.||..|.|.||.|||.|.:
Mouse   755 QRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEK 819

  Fly  5105 DDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5149
            ..:. :.||.:|||||:||||.||||::|:..||.||.| :||||
Mouse   820 VGKE-NWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 862

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:283630
		DUF913	621..989	CDD:283642
		UBA_like_SF	1467..1506	CDD:304366
		WWE	1756..1828	CDD:128922
		40S_SA_C	3575..3651	CDD:292740
		DUF4414	3675..3779	CDD:291075
		HECTc	4796..5149	CDD:238033	154/352 (44%)
		HECTc	4820..5148	CDD:214523	144/327 (44%)
Itch	NP_001230641.1	C2_E3_ubiquitin_ligase	17..140	CDD:175988	28/177 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	151..294		26/144 (18%)
		WW	289..318	CDD:278809	2/28 (7%)
		WW	321..350	CDD:278809	0/28 (0%)
		Required for interaction with FYN. /evidence=ECO:0000250	356..432		13/80 (16%)
		WW	401..430	CDD:278809	3/28 (11%)
		WW	440..471	CDD:197736	7/41 (17%)
		HECTc	509..862	CDD:238033	154/354 (44%)
		HECTc	533..861	CDD:214523	144/329 (44%)
		MAP kinase docking site	535..544		2/8 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810