Protein Alignment HUWE1 and WWP1

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: NP_008944.1
• Gene: WWP1 / 11059
• HGNCID: 17004
• Length: 922
• Species: Homo sapiens
• Alignment Length: 1006
• Identity: 276/1006 - (27%)
• Similarity: 437/1006 - (43%)
• Gaps: 223/1006 - (22%)

  Fly  4280 ASGNPASGDSNAGAKRTQLPTLSVALPVTPSKPRRKIYSKNFSQLQLVIEVL-------THQCGT 4337
            |:.:|.|..||..:.|.||     .:.|:.:|.:||   ||:....:..||:       |.:..:
Human     2 ATASPRSDTSNNHSGRLQL-----QVTVSSAKLKRK---KNWFGTAIYTEVVVDGEITKTAKSSS 58

  Fly  4338 TEGLDNVAKLIVNLTQCSQASSAIFI-QYLTA-AILGLA---------------EEVRQAIQMLL 4385
            :.......:|.||:|..:.....::. :.|.| |:||.|               |.|::.:::.|
Human    59 SSNPKWDEQLTVNVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVKEQLKLSL 123

  Fly  4386 NE------------------IRMYNI------------------HQGG---AGTTSQAA--STSG 4409
            ..                  |...||                  |:.|   |.||::.|  .|:|
Human   124 ENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNG 188

  Fly  4410 AAAATASYVPTVSGASLLPNLAVHEGIMQDRFTAEHVIISA-PKNAKPTCELQLPSMKKLMSNSS 4473
                ..::|||   ::|:.|......:..|...:....::| |||.        |:.|.|.|..:
Human   189 ----IDNHVPT---STLVQNSCCSYVVNGDNTPSSPSQVAARPKNT--------PAPKPLASEPA 238

  Fly  4474 AQPYFLRTLKIFMQVRDMYEAQNGLSTGGGVACGAGDNDDDIIDIEGDGGASGGSSSDMAPGSAS 4538
            .                  :..||.|:.......|......::..|          :.::|...|
Human   239 D------------------DTVNGESSSFAPTDNASVTGTPVVSEE----------NALSPNCTS 275

  Fly  4539 TISPPPPMETNQIAQDGDDDE-------EDDDEARSF--PGVGNHAVAS---ASKSTKPTLSQIL 4591
            |....||::  :|....:::|       |.:.||||.  |...|...:|   |:||.:|.    .
Human   276 TTVEDPPVQ--EILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAAKSRQPD----G 334

  Fly  4592 CLDVL----WNTLSECLVA-LEESKDEFAVLVLQPTVEAFFLIH--------------------- 4630
            |:|.:    .|..:|.|.: .|:.||        |....:::.|
Human   335 CMDPVRQQSGNANTETLPSGWEQRKD--------PHGRTYYVDHNTRTTTWERPQPLPPGWERRV 391

  Fly  4631 --------ASHRGASKKSGRSFMPNAAGF--------GLRAAAGAGAIGGLGAAAPSAQEYSPF- 4678
                    ..|...:....|..|.:...|        .|:.|........|.:|:..|.|..|:
Human   392 DDRRRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYG 456

  Fly  4679 ------------MDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQK 4731
                        .|.|...|..:..:..::||.|......||           ....::|..|:.
Human   457 PLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEEPL-----------PEGWEIRYTREG 510

  Fly  4732 FLQFAEKH-RTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQ--TELERLDEGIRREE 4793
            ...|.:.: ||...:..|...:.::.|...:..:.    .|..|..:|:  .:...|...::
Human   511 VRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYER----GFRWKLAHFRYLCQSNALPSHVK--- 568

  Fly  4794 HTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALF 4858
              ::|.|.|:|||||:.:..|.|.:.:.|.|::|..|||.|.|||.|||:.::|.|:.||||.||
Human   569 --INVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLF 631

  Fly  4859 CVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDM 4923
            ..:..:.....|||:|..||:|||||.|:||.||.|:...|.::..|:..|||.:|.|::...|:
Human   632 EYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDL 696

  Fly  4924 ESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVC 4988
            ||.|.|||..|.::..|:|...|.|:.||.:::..|.....|||..|.:..||||||.||:.|:.
Human   697 ESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMT 761

  Fly  4989 QLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSA 5053
            :.:.|..:::|..|||:||.:::|...:..|:|:|||:::.|:.::|:.|.:.||.|..||..|.
Human   762 EWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSK 826

  Fly  5054 QIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTC 5118
            ||.|||:.::..|...|.:.|||||||.::||.||..|.|.||.|||.|.:..:.| .||.:|||
Human   827 QIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDT-WLPRSHTC 890

  Fly  5119 FNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5149
            ||:||||.||||::|:..||.||.| :||||
Human   891 FNRLDLPPYKSYEQLKEKLLFAIEE-TEGFG 920

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159
		HECTc	4796..5149	CDD:238033	155/352 (44%)
WWP1	NP_008944.1	C2_E3_ubiquitin_ligase	17..140	CDD:175988	25/130 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	210..388		43/227 (19%)
		SP1-4_N	<236..>344	CDD:425404	26/141 (18%)
		HUL4	<307..922	CDD:227354	207/648 (32%)
		Required for interaction with and ubiquitination of AMOTL2. Required for interaction with YAP1. /evidence=ECO:0000269|PubMed:34404733	349..531		32/200 (16%)