Protein Alignment Cngl and hcn4

DIOPT Version :9

• Sequence 1: NP_001245691.1
• Gene: Cngl / 32468
• FlyBaseID: FBgn0263257
• Length: 1970
• Species: Drosophila melanogaster
• Sequence 2: XP_686078.4
• Gene: hcn4 / 557843
• ZFINID: ZDB-GENE-050420-360
• Length: 1140
• Species: Danio rerio
• Alignment Length: 1284
• Identity: 298/1284 - (23%)
• Similarity: 490/1284 - (38%)
• Gaps: 364/1284 - (28%)

  Fly     9 RAHASAHKKILSSGYHSQLNYGSTGAAAASSS-------------SSGATATGLYTMEAHEHEAG 60
            |.|:|..|::.|...|........|.|..|..             :||...:|  .....|.:.|
Zfish     3 RLHSSMRKRLYSLPQHIGQKASIMGDAEDSDKDTRRKSIRMKPLPTSGEIKSG--ESAIMETDIG 65

  Fly    61 KFVKD----------------VARQVHDCNSD--------TD-VISPTGTSISGGGGGGGAGGAG 100
            :.||.                .:|.|||  ||        || .::|:..|..|..|||...||
Zfish    66 RPVKTSSNGDCRRFRGSLSSITSRHVHD--SDVAEERRLITDGEVTPSEESPPGAAGGGAEPGA- 127

  Fly   101 GSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPSQRKSRIG-DGASD--PDSDWTRSNQ 162
            .|||.||....|                |:.|..   .|.|:...|. :|...  ||.:  |..|
Zfish   128 QSGGGGGEEAAG----------------SSQHQG---IPDQQAGFIKLEGIEQILPDDE--RYYQ 171

  Fly   163 RWMKLRTTVQISSAIQKKPPLKREDSFLKRFSTRQIPETQETVEDTGSESASGDVDKSVKRRRRY 227
            .....|   |..:.:|..         :.:||.|..          |||       |:|:|.:..
Zfish   172 AGFMHR---QFGAMLQPG---------VNKFSLRMF----------GSE-------KAVEREQER 207

  Fly   228 LQKRRS-VVNPDENFYFYWLMMLTVCVLYNLWTLIVRQSFPELQQSVPTFWLICDSMTDVVFILD 291
            ::.... :::|..:|.|||.:.:.:.::.||..:.|..:|.:.:.:.|  |::.:.::|..|::|
Zfish   208 VKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDEHTPP--WIVFNVVSDTFFLMD 270

  Fly   292 IIVQLRTGYLEQ--GLMVYDDRKLACHYVHSRDFIFDMIALIPLDLLQLKMGTH----------- 343
            :::..|||.:::  ..::.|.:::...|:.|. |:.|.|:.||:|.:.|.:.|.
Zfish   271 LVLNFRTGIVKEDNAEIILDPQQIKIKYLRSW-FVVDFISSIPVDYIFLIVETRIDSDFYKTARA 334

  Fly   344 -PLLRFTRFFKVYRSVRFYYIVESRTVWPNLW-----------RVVNLIHILLILAHWFGCFYFL 396
             .::|||:...:.|.:|...::.....|..::           |:||||.::|:|.||.||..||
Zfish   335 LRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAMVRIVNLIGMMLLLCHWDGCLQFL 399

  Fly   397 LSEAEGFQGD-WVYPYRPGDYATLTRKYLGSLYWSTLTLTTIG-DLPTPETNAEYIFTIVSYLIG 459
            :...:.|..| ||...:..: .|..::|..:|:.:...:..|| .:..|....:...||:|.::|
Zfish   400 VPMLQDFPADCWVAKNKMVN-DTWGQQYSYALFKAMSHMLCIGYGMYPPVGMTDVWLTILSMIVG 463

  Fly   460 VFIFATIVGQVGNVITNRNANRLEFERLLDGAKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGG 524
            ...:|..||....:|.:.:::|.:::......:.||..||:|..|::|:..:|::     |.||.
Zfish   464 ATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQRIHDYYEH-----RYQGK 523

  Fly   525 -GDINTALGLLPDKLKTELALHVNLSVLKKVT---IFQECQPEFLHDLVLKMKAYIFTPGDSICR 585
             .|..:.||.|.:.|:.|:   :|.:..|.|.   :|....|.|:..::.|::..:|.|||.|.|
Zfish   524 MFDEESILGELNEPLREEI---INFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIR 585

  Fly   586 KGEVAREMFIIADGILEVLSETGKVLTTMKAGDFFGEIGILNLDGLNKRTADVRSVGYSELFSLS 650
            :|.:.::|:.|..|::.||:: |...|.:..|.:||||.:|.   ..:|||.||:..|..|:|||
Zfish   586 EGTIGKKMYFIQHGVVSVLTK-GNKETKLSDGSYFGEICLLT---RGRRTASVRADTYCRLYSLS 646

  Fly   651 REDVLAAMKDYPDAQEILQTLGRKRLMEVRCVNKKYA----KAQSD--------KEAAAYAAAHP 703
            .::....:::||..:...:|:...||..   :.||.:    |.|.|        :|:........
Zfish   647 VDNFNEVLEEYPMMRRAFETVALDRLDR---IGKKNSILQHKVQHDLNSGVLNYQESEIIQQIVQ 708

  Fly   704 HHHQSHHQGQVHQSDS--------------------SENSASKKIVDKLKH-------------D 735
            |.....|...:.||.|                    ...:|:..:...|.|             .
Zfish   709 HDRDMAHCANLLQSPSPPAPPSPTPVIWAPLIQAPLQAAAATTSVAIALTHHPHLPATLFRPPVS 773

  Fly   736 VKGFRNV----LKKSRTSRKSDESLEMQPLHNTSPRGSKILLKRMSRVRSDEKDADSAEAKDELH 796
            :.|.||.    ||:.:|......|....|  :|||                          .:||
Zfish   774 LLGSRNEPPSRLKRFQTVAPRTGSTTDSP--STSP--------------------------SKLH 810

  Fly   797 DKTPSPIGAGLPLLQRLRLLKEKQDREERAVKSTP---------------PQKSPPHSHVTCTLS 846
            .      |...|||..|.       .:..:|..||               |..||..|  |..|.
Zfish   811 S------GVDTPLLASLH-------TQHSSVTITPSATTNQPVSFRSFSSPSASPTLS--TAQLH 860

  Fly   847 PQESIQEEPEREFSEGFPLIQRLQQLKIKNEPQANAAEPGVVMV---NT---------------- 892
            ||.. |::|...     ||..|||        .|.|..||::..   ||
Zfish   861 PQPR-QKQPSTP-----PLSARLQ--------AAGAHPPGILTTASSNTSALSAGLSTHSPTHSY 911

  Fly   893 PPNISSKIDSHFAASQAGAS-----HPGPNGGAIGI--SAPGQSLTVAQIKP------IMKVSFK 944
            ||....:..|...||..|.|     |..|.|..|.|  .|| :|.|.:|.:|      .::.|..
Zfish   912 PPPKHPQTGSLQFASGGGKSGLTLFHSPPPGSPISIHSQAP-ESSTSSQTQPSFSSYLALERSAL 975

  Fly   945 QKIQQMQGGGGGASSSP------------GPSTGAIAKKEPPKSLA----------------LVA 981
            ..|.|.    |.|::||            .|.||...:...|.|.|                |..
Zfish   976 ASIAQY----GSANASPSYTPLALSPTVQSPVTGRTFQYSDPSSAAGSHTSLLLPQSSCPGQLPG 1036

  Fly   982 KHATSED-PV------------AAAGLGL-GSGSSSG-------IATISKRVVKPQSHRMATPLQ 1025
            .|::..| |:            :...||: |:|.||.       ..|::.:.....|..|::|:|
Zfish  1037 HHSSGTDSPLGRFYEDLNILSSSHPSLGVEGAGQSSPGYLSPYLSPTLTPKPCSSVSGHMSSPVQ 1101

  Fly  1026 SDTDTDGTPIKPWSKLKLATLMSSSYTSLTNCSPDDLASPLKNYSLSNI 1074
            :         .|..:.:..::.:|...||...|..|| .||::...||:
Zfish  1102 T---------SPGPRGQALSVGTSPALSLPRTSDGDL-EPLRSKLPSNL 1140

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cngl	NP_001245691.1	Ion_trans	260..481	CDD:278921	58/247 (23%)
		Ion_trans_2	382..476	CDD:285168	26/95 (27%)
		Crp	550..>680	CDD:223736	41/132 (31%)
		CAP_ED	556..668	CDD:237999	36/111 (32%)
hcn4	XP_686078.4	Ion_trans_N	179..221	CDD:285597	11/67 (16%)
		Ion_trans	241..486	CDD:278921	58/248 (23%)
		Crp	550..>685	CDD:223736	42/141 (30%)
		CAP_ED	556..664	CDD:237999	36/111 (32%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170578311
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.840