Protein Alignment Cngl and Hcn1

DIOPT Version :9

• Sequence 1: NP_001245691.1
• Gene: Cngl / 32468
• FlyBaseID: FBgn0263257
• Length: 1970
• Species: Drosophila melanogaster
• Sequence 2: NP_034538.2
• Gene: Hcn1 / 15165
• MGIID: 1096392
• Length: 910
• Species: Mus musculus
• Alignment Length: 1076
• Identity: 237/1076 - (22%)
• Similarity: 426/1076 - (39%)
• Gaps: 301/1076 - (27%)

  Fly    76 DTDVISPTGTSISGGGGGGGAGGAGGSGGAGGHSDGGYHKRHHHKMVRDDDNNSAAHSSDSKSPS 140
            |.:.:.|:....:|........|....|||.|...|                ||.....|
Mouse    16 DGNSVFPSKAPATGPVAADKRLGTPPGGGAAGKEHG----------------NSVCFKVD----- 59

  Fly   141 QRKSRIGDGASDP-----DSDWTRSNQRWMKLRTTVQISSAIQKKPPLKREDSFLKRFSTRQIPE 200
                  |.|..:|     |::..|....:|:.:.|..:...:.|             ||.|..
Mouse    60 ------GGGGEEPAGSFEDAEGPRRQYGFMQRQFTSMLQPGVNK-------------FSLRMF-- 103

  Fly   201 TQETVEDTGSESASGDVDKSVKRRRRYLQKRRSVVNPDENFYFYWLMMLTVCVLYNLWTLIVRQS 265
                    ||:.|.....:.||....:      :::|..:|.|||.:::.:.::.||..:.|..:
Mouse   104 --------GSQKAVEKEQERVKTAGFW------IIHPYSDFRFYWDLIMLIMMVGNLVIIPVGIT 154

  Fly   266 FPELQQSVPTFWLICDSMTDVVFILDIIVQLRTGYL--EQGLMVYDDRKLACHYVHSRDFIFDMI 328
            |...|.:.|  |:|.:..:|.||:||:|:..|||.:  :...::.|.:.:..:|:.|. |:.|.|
Mouse   155 FFTEQTTTP--WIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSW-FVVDFI 216

  Fly   329 ALIPLD--LLQLKMGTHP----------LLRFTRFFKVYRSVRFYYIVESRTVWPNLW------- 374
            :.||:|  .|.::.|...          ::|||:...:.|.:|...::.....|..::
Mouse   217 SSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLA 281

  Fly   375 ----RVVNLIHILLILAHWFGCFYFLLSEAEGFQGD-WVYPYRPGDYATLT--------RKYLGS 426
                |:.|||.::|:|.||.||..||:...:.|..| ||         :|.        ::|..:
Mouse   282 SAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWV---------SLNEMVNDSWGKQYSYA 337

  Fly   427 LYWSTLTLTTIG-DLPTPETNAEYIFTIVSYLIGVFIFATIVGQVGNVITNRNANRLEFERLLDG 490
            |:.:...:..|| ....|.:.::...|::|.::|...:|..||....:|.:.:::|.:::.....
Mouse   338 LFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQ 402

  Fly   491 AKTYMRHHKVPGGMKRRVLRWYDYSWSRGRIQGGGDINTALGLLPDKLKTELALHVNLSVLKKVT 555
            .:.||..||:|..|::::..:|::.: :|:|   .|....|..|.|.|:.|:   ||.:..|.|.
Mouse   403 VEQYMSFHKLPADMRQKIHDYYEHRY-QGKI---FDEENILSELNDPLREEI---VNFNCRKLVA 460

  Fly   556 ---IFQECQPEFLHDLVLKMKAYIFTPGDSICRKGEVAREMFIIADGILEVLSETGKVLTTMKAG 617
               :|....|.|:..::.|::..:|.|||.|.|:|.|.::|:.|..|:..|::::.|.: .:..|
Mouse   461 TMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEM-KLTDG 524

  Fly   618 DFFGEIGILNLDGLNKRTADVRSVGYSELFSLSREDVLAAMKDYPDAQEILQTLGRKRLMEVRCV 682
            .:||||.:|.   ..:|||.||:..|..|:|||.::....:::||..:...:|:...||..   :
Mouse   525 SYFGEICLLT---KGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDR---I 583

  Fly   683 NKKYA----KAQSDKEAAAYAAAHPHHHQSHHQGQVHQSDSSENSASKKIVDKLKHDVKGFRNVL 743
            .||.:    |.|.|.....:                   ::.||...|:||   |||
Mouse   584 GKKNSILLQKFQKDLNTGVF-------------------NNQENEILKQIV---KHD-------- 618

  Fly   744 KKSRTSRKSDESL------EMQPLHNTSPRGSKILLKRMSRVRSDEKDADSAE--AKDELHDKTP 800
                  |:..:::      :|..|:.||...:.     .||:|:......:|.  :...||..:|
Mouse   619 ------REMVQAIPPINYPQMTALNCTSSTTTP-----TSRMRTQSPPVYTATSLSHSNLHSPSP 672

  Fly   801 S----------------------PIGAGL---------PLLQRLRLLKEKQDREERAVKSTPPQK 834
            |                      ||.:.|         |...:|.|:::             ||:
Mouse   673 STQTPQPSAILSPCSYTTAVCSPPIQSPLATRTFHYASPTASQLSLMQQ-------------PQQ 724

  Fly   835 SPPHSHV----TCTLSPQESIQEEPEREFSEGFPLIQRLQQLKIKNEPQANAAEPGVVMVNTPPN 895
            ..|.|.|    |.|...|:..|::.:::..:.....|:.||.:.:.:.|.....||   .:||.|
Mouse   725 QLPQSQVQQTQTQTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPQTPG---SSTPKN 786

  Fly   896 ISSKIDSHFAASQAGASHPGPNGGAIGISAPGQSLTVAQIKPI--MKVSFKQKIQQMQGGGGGAS 958
            ...|      ::||               ....:|| .:::|:  .:.|...::..:       .
Mouse   787 EVHK------STQA---------------LHNTNLT-KEVRPLSASQPSLPHEVSTL-------I 822

  Fly   959 SSPGPSTGAIAKKEPPKSLALVAKHATSEDPVAAA-GLGLGSGSSSGIATISKRVV--------- 1013
            |.|.|:.|        :|||.:.:      ||||. ..||.:||.|   |:.:||.
Mouse   823 SRPHPTVG--------ESLASIPQ------PVAAVHSTGLQAGSRS---TVPQRVTLFRQMSSGA 870

  Fly  1014 ------------KPQSHRMATPLQSDTDTDGTPIKP 1037
                        .|.:.:..:|...:||.|..  ||
Mouse   871 IPPNRGVPPAPPPPAAVQRESPSVLNTDPDAE--KP 904

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cngl	NP_001245691.1	Ion_trans	260..481	CDD:278921	61/255 (24%)
		Ion_trans_2	382..476	CDD:285168	25/103 (24%)
		Crp	550..>680	CDD:223736	40/132 (30%)
		CAP_ED	556..668	CDD:237999	35/111 (32%)
Hcn1	NP_034538.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..75		16/85 (19%)
		Ion_trans_N	87..129	CDD:285597	11/70 (16%)
		Ion_trans	149..394	CDD:278921	61/256 (24%)
		Selectivity filter. /evidence=ECO:0000305|PubMed:12089064	347..351		2/3 (67%)
		Crp	458..>593	CDD:223736	41/141 (29%)
		CAP_ED	464..572	CDD:237999	35/111 (32%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	634..681		11/51 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	771..791		7/28 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	865..910		7/42 (17%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167835144
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	217	1.000	Inparanoid score	I3583
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.890