DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top1 and TOP1

DIOPT Version :9

Sequence 1:	NP_001014742.1	Gene:	Top1 / 32458	FlyBaseID:	FBgn0004924	Length:	974	Species:	Drosophila melanogaster
Sequence 2:	NP_014637.1	Gene:	TOP1 / 854156	SGDID:	S000005366	Length:	769	Species:	Saccharomyces cerevisiae

Alignment Length:	776	Identity:	327/776 - (42%)
Similarity:	447/776 - (57%)	Gaps:	133/776 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly   321 DADEMN-----DDEEDVPLA--MRKRK---QEATDRPDGGMDNDDDDDIPLLARKKVKKEK---- 371
            ||.::|     ||::||||:  ::|||   ..:....|.....|.|:.|..:::||.||.|
Yeast     5 DASKVNHELSSDDDDDVPLSQTLKKRKVASMNSASLQDEAEPYDSDEAISKISKKKTKKIKTEPV 69

  Fly   372 ---------IKKESKEKSKKRVKEEPSDDYGNVKPK-KKKMKKEPEPAVSPGKRQKAKAKVEEEE 426
                     .||.:..|.||..||:     |:||.| .||.::|.|......:.::.|...||||
Yeast    70 QSSSLPSPPAKKSATSKPKKIKKED-----GDVKVKTTKKEEQENEKKKREEEEEEDKKAKEEEE 129

  Fly   427 VWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNH 491
            .::|||:|...|.:||.||:|.|.:|.|.|:.:|.:::.||||||::|..:.||.|.|:|.:|..
Yeast   130 EYKWWEKENEDDTIKWVTLKHNGVIFPPPYQPLPSHIKLYYDGKPVDLPPQAEEVAGFFAALLES 194

  Fly   492 DYCTKEVFNNNFFKDF----RKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIK 552
            |:....||..|||.||    ::|..|.....||:|.:|:|.:||:|||.:.|::|..:.:||...
Yeast   195 DHAKNPVFQKNFFNDFLQVLKESGGPLNGIEIKEFSRCDFTKMFDYFQLQKEQKKQLTSQEKKQI 259

  Fly   553 KNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKV 617
            :.|.|...:::.||.:||.:|::|||::|||.||||||.|||.|.:|||:...|:.:|..||:.|
Yeast   260 RLEREKFEEDYKFCELDGRREQVGNFKVEPPDLFRGRGAHPKTGKLKRRVNPEDIVLNLSKDAPV 324

  Fly   618 PSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKI 682
            |..|.|.:|.|:||||||.|||.|.||:....||:.|..:|.|||:.|:.|:|.||:|...||.|
Yeast   325 PPAPEGHKWGEIRHDNTVQWLAMWRENIFNSFKYVRLAANSSLKGQSDYKKFEKARQLKSYIDAI 389

  Fly   683 RATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKEN 747
            |..|....|||.|..||:|||:|.||..|||||.||.||:||||||||||.|||.|.     ..|
Yeast   390 RRDYTRNLKSKVMLERQKAVAIYLIDVFALRAGGEKSEDEADTVGCCSLRYEHVTLK-----PPN 449

  Fly   748 VVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKE-GDDLFDRLNTQVLNEHLKELMEGLTAK 811
            .|:|||.||||||:|.||||:|:|||||.:|....|: |..|||||:..:||::|:..|.|||||
Yeast   450 TVIFDFLGKDSIRFYQEVEVDKQVFKNLTIFKRPPKQPGHQLFDRLDPSILNKYLQNYMPGLTAK 514

  Fly   812 VFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLK---- 872
            ||||||||||:|.||||:.: ..:|.||:|.||.|||.|||||||||:|.|.|.:::|...
Yeast   515 VFRTYNASKTMQDQLDLIPN-KGSVAEKILKYNAANRTVAILCNHQRTVTKGHAQTVEKANNRIQ 578

  Fly   873 ----EKIKAKREAIE------KCESEY---------------HSR-------------------- 892
                :||:.||..::      |.|.:|               |.|
Yeast   579 ELEWQKIRCKRAILQLDKDLLKKEPKYFEEIDDLTKEDEATIHKRIIDREIEKYQRKFVRENDKR 643

  Fly   893 --------------------DEKKGK-----------------QLERLRDQLKKLE-------LQ 913
                                ||||.:                 .:|:::.|::|||       :|
Yeast   644 KFEKEELLPESQLKEWLEKVDEKKQEFEKELKTGEVELKSSWNSVEKIKAQVEKLEQRIQTSSIQ 708

  Fly   914 ETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 974
            ..|::||..::|||||:||:|||:||.:|||:||||||||.||.|.||.||:...|||:||
Yeast   709 LKDKEENSQVSLGTSKINYIDPRLSVVFCKKYDVPIEKIFTKTLREKFKWAIESVDENWRF 769

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top1	NP_001014742.1	Topoisomer_IB_N_htopoI_like	441..655	CDD:239570	97/217 (45%)
		TOPEUc	585..946	CDD:214661	201/454 (44%)
		Topoisom_I	657..859	CDD:279380	121/202 (60%)
		Topo_C_assoc	905..972	CDD:291068	40/73 (55%)
TOP1	NP_014637.1	Topoisom_I_N	143..361	CDD:397183	97/217 (45%)
TOP1	NP_014637.1	TOPEUc	292..741	CDD:214661	201/454 (44%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346278
Domainoid	1	1.000	239	1.000	Domainoid score	I375
eggNOG	1	0.900	-	-		E1_COG3569
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2467
Inparanoid	1	1.050	512	1.000	Inparanoid score	I200
Isobase	1	0.950	-	0	Normalized mean entropy	S549
OMA	1	1.010	-	-		QHG62324
OrthoFinder	1	1.000	-	-		FOG0001157
OrthoInspector	1	1.000	-	-		oto100336
orthoMCL	1	0.900	-	-		OOG6_101409
Panther	1	1.100	-	-	LDO	PTHR10290
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2314
SonicParanoid	1	1.000	-	-		X783
TreeFam	1	0.960	-	-
	16	15.740