DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top1 and TOP1BETA

DIOPT Version :9

Sequence 1:	NP_001014742.1	Gene:	Top1 / 32458	FlyBaseID:	FBgn0004924	Length:	974	Species:	Drosophila melanogaster
Sequence 2:	NP_200342.1	Gene:	TOP1BETA / 835624	AraportID:	AT5G55310	Length:	917	Species:	Arabidopsis thaliana

Alignment Length:	994	Identity:	351/994 - (35%)
Similarity:	506/994 - (50%)	Gaps:	138/994 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 VTTNGHGHHHHHHHSSSSSSSSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRD--KDRHKS 89
            |..|||..:.............::.:::...:|.....:...||:..|.:.....|.  ...::|
plant    10 VVPNGHDGYEDEDEDDIPLVFKRNSNTAATTNRPSPINNAMRNSAIGSTKSSPPMRSPLTSPNRS 74

  Fly    90 SSSSSKHRDKDKERDGSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGS 154
            :|||::..........||:..||...|..:| |.|..:|.::..|.....||..   |:|.:...
plant    75 ASSSTRSSMMKPALPSSSSVQRSTLKSPLRD-DRSVVAKERNGFGKAPSVSKSD---DEDSEDDK 135

  Fly   155 SSSSRHKSSSSSRDKERSSSSHKSSSS------------SSSSKSKHSSSRHSSSSSSKDQPSYD 207
            ..|:|.|..|....|:.|||...|:..            |..:|.|....|...||:.:.:.|..
plant   136 PLSARLKLDSKEVTKQPSSSGRGSTQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVG 200

  Fly   208 GVFVKPEPVSQQLMHSGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEE 272
            ....||..:.|:.....::|                              :||       :|.:.
plant   201 TSSSKPVHIEQKRPLVNNID------------------------------RNG-------LKPKT 228

  Fly   273 ESFNNLSQA--------SSCDYSMSQFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDE 329
            |..::.:.|        ||...|:.:.:..||...||.||.        ::.....||...|.|.
plant   229 EGHSSQAPAKRPLEKGSSSNQSSVKRPKLSEPARPVKVEQG--------SHISATQDAKGKNLDA 285

  Fly   330 EDVPLAMRKRKQEATDRPDGGMDNDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGN 394
            .. ||    |..:||.:.|   ::|.||.:|:.:|.|......|..|.:.|..::
plant   286 SK-PL----RANQATVKED---NSDGDDHVPIASRMKSDSSNNKSSSAKPSSSKM---------- 332

  Fly   395 VKPKKKKMKKEPEPAVSPGKRQKAKAKVEEEEVWRWWEEEKRADG-VKWSTLEHKGPVFAPRYER 458
            :....:.:.::|...|...|..|:...:..            .|| .||.||:|.|.:|.|.|:|
plant   333 IASSSRTIAQKPNKWVKDSKYSKSSKSLPS------------GDGQKKWKTLQHNGVIFPPPYKR 385

  Fly   459 VPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFR 523
              ..|:..:.|||::|:.|.||.||.:|.|...:|..|..|..||:.|:||.:  .:..:||...
plant   386 --HGVKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLL--GKNHMIKSLD 446

  Fly   524 KCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRG 588
            .|:|..::.::..|.|.:|..:.|||.|.|.|.....:::.:.::||.:|:|||||:||||||||
plant   447 DCDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFRG 511

  Fly   589 RGEHPKMGMIKRRIQASDVSINCGKDSKVPS-PPPGSRWKEVRHDNTVTWLASWIENVQ-GQVKY 651
            ||||||||.:|:||:..|::||.||::.:|. |.||.|||||:|||||||||.|.:.:. .:.||
plant   512 RGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPINPKEFKY 576

  Fly   652 IMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGN 716
            :.|..||.|||:.|..|||.||:|...|..|||.|..::.:|::..||.|||.|.||||||||||
plant   577 VFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAGN 641

  Fly   717 EKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEH 781
            |||:|:|||||||:|:|.:|:..     ..|.:.|||.|||||:|.|.||||..|:|.:..|...
plant   642 EKDDDEADTVGCCTLKVGNVECI-----PPNKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAG 701

  Fly   782 KKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRA 846
            |.:.|||||.|:|..||.||||||.|||||||||||||.||...|...| ....||||::.|.:|
plant   702 KSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKET-RDGDVPEKVVVYQQA 765

  Fly   847 NRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKKG-------------- 897
            |:.|||:|||||:|.|||...:|.|..||:..||.|::...:...  .|||
plant   766 NKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDR--AKKGRTPLMGSDGKRKRN 828

  Fly   898 -------KQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNK 955
                   |::.:.:.:::|:|.....:::.||:||||||:||:||||:|||||:|||||||||||
plant   829 LTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITVAWCKRHDVPIEKIFNK 893

  Fly   956 TQRTKFLWAVHMADENYRF 974
            :...||.||:.: |..:||
plant   894 SLLAKFAWAMDV-DPEFRF 911

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top1	NP_001014742.1	Topoisomer_IB_N_htopoI_like	441..655	CDD:239570	96/215 (45%)
		TOPEUc	585..946	CDD:214661	192/383 (50%)
		Topoisom_I	657..859	CDD:279380	113/201 (56%)
		Topo_C_assoc	905..972	CDD:291068	37/66 (56%)
TOP1BETA	NP_200342.1	Topoisom_I_N	367..579	CDD:367250	96/215 (45%)
TOP1BETA	NP_200342.1	TOPEUc	508..884	CDD:214661	192/383 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	226	1.000	Domainoid score	I677
eggNOG	1	0.900	-	-		E1_COG3569
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2467
Inparanoid	1	1.050	530	1.000	Inparanoid score	I281
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D303947at2759
OrthoFinder	1	1.000	-	-		FOG0001157
OrthoInspector	1	1.000	-	-		otm2698
orthoMCL	1	0.900	-	-		OOG6_101409
Panther	1	1.100	-	-	LDO	PTHR10290
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X783
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	14	13.830