DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top1 and TOP1ALPHA

DIOPT Version :9

Sequence 1:	NP_001014742.1	Gene:	Top1 / 32458	FlyBaseID:	FBgn0004924	Length:	974	Species:	Drosophila melanogaster
Sequence 2:	NP_001190543.1	Gene:	TOP1ALPHA / 835623	AraportID:	AT5G55300	Length:	933	Species:	Arabidopsis thaliana

Alignment Length:	977	Identity:	349/977 - (35%)
Similarity:	506/977 - (51%)	Gaps:	131/977 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    52 SSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSKHRDKDKERDGSSNSHR----- 111
            :.|.|...|:....|.:|:.:|:.....|:..:.:...::..|..|......:|::.|::
plant    13 NGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMRSPVTSPNGTTPSNKTSIVK 77

  Fly   112 -SGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDK-------ERRDKDKDRGSSS---------SSR 159
             |..|||.|.....|..::...|....||...|       |..|.:.||..||         |||
plant    78 SSMPSSSSKASPAKSPLRNDMPSTVKDRSQLQKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSR 142

  Fly   160 -------HKSSSSSRDKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDQPSYDGVFVKPEPVS 217
                   .|.::..|..:|:|...|..|...:..|.....:..|..|..:|.|.|    :.:|:.
plant   143 QVSSPQPEKKNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSND----EKKPLV 203

  Fly   218 QQLMHSGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNLSQAS 282
            |:|..:||....::.                ||.|.|              |:..|..::..|:
plant   204 QKLHQNGSTVKNEVP----------------NGKVLG--------------KRPLEKNSSADQS- 237

  Fly   283 SCDYSMSQFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAMRKRKQEATDRP 347
                |:.:.:....|..||.:|               |...:..||:..|.::.:.:.::.:.|.
plant   238 ----SLKKAKISASPTSVKMKQ---------------DSVKKEIDDKGRVLVSPKMKAKQLSTRE 283

  Fly   348 DGGMDNDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGNVKPKKKKMKKEPEPAVSP 412
            | |.|:|||||:|:  .|:.|.:.....:.....|.||...:.  ...|||.:.:       |||
plant   284 D-GTDDDDDDDVPI--SKRFKSDSSNSNTSSAKPKAVKLNSTS--SAAKPKARNV-------VSP 336

  Fly   413 GKRQKAK--AKVEEEEVWRWWEEEKRADG---VKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPL 472
            ..|...|  .||.::..:....:...:.|   .||:||.|.|.:|.|.|:  |..::..|.|||:
plant   337 RSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYK--PHGIKILYKGKPV 399

  Fly   473 ELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAE 537
            :|:.|.||.||.:|.|...||.||..|..||:.|:|:.:  .::.:|:....|:|..::.:...|
plant   400 DLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLL--GKKHVIQKLDDCDFTPIYEWHLEE 462

  Fly   538 SEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRI 602
            .||:|..|.|||...|.|.....:::.:.::||.||||||||:||||||||||||||||.:|:||
plant   463 KEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGRGEHPKMGKLKKRI 527

  Fly   603 QASDVSINCGKDSKVPS-PPPGSRWKEVRHDNTVTWLASWIENVQ-GQVKYIMLNPSSKLKGEKD 665
            ...::::|.||.:.:|. |..|.|||||:|||||||||.|.:.:. .:.||:.|...|.|||..|
plant   528 HPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFLGAGSSLKGLSD 592

  Fly   666 HIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCS 730
            ..|||.||.|...||.||.||...:.:|::::||.|||.|.|||||||||||||:|:|||||||:
plant   593 KEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRAGNEKDDDEADTVGCCT 657

  Fly   731 LRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQ 795
            |:|.:|:..     ..|.:.|||.|||||:|.|.||||..|:|.:..|...|.:.|||||.|:|.
plant   658 LKVGNVECI-----PPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKSKTDDLFDELDTS 717

  Fly   796 VLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSV 860
            .||.|||||:.|||||||||||||.||...|...| ....|.:|::.|.:||:.|||:|||||:|
plant   718 KLNAHLKELVPGLTAKVFRTYNASITLDEMLSQET-KDGDVTQKIVVYQKANKEVAIICNHQRTV 781

  Fly   861 PKSHEKSMENLKEKIKAKREAIEKCESE----------YHSRDEKKGKQLE------RLRDQLKK 909
            .|:|...:|.|..:|:..:|.:::.::.          ....|.||.:.||      ::..|..|
plant   782 SKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAK 846

  Fly   910 LELQETD---RDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADEN 971
            :|..|.|   :::.||:||||||:|||||||:|||||:|:|||||||.|:...||.||:.:..|.
plant   847 IEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFTKSLLEKFAWAMDVEPEA 911

  Fly   972 YR 973
            .|
plant   912 KR 913

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top1	NP_001014742.1	Topoisomer_IB_N_htopoI_like	441..655	CDD:239570	96/215 (45%)
		TOPEUc	585..946	CDD:214661	187/381 (49%)
		Topoisom_I	657..859	CDD:279380	110/201 (55%)
		Topo_C_assoc	905..972	CDD:291068	39/69 (57%)
TOP1ALPHA	NP_001190543.1	Topoisomer_IB_N_LdtopoI_like	370..582	CDD:239571	96/215 (45%)
		TOPEUc	510..886	CDD:214661	187/381 (49%)
		Topoisom_I	585..780	CDD:279380	110/200 (55%)
		Topo_C_assoc	846..910	CDD:291068	37/63 (59%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	226	1.000	Domainoid score	I677
eggNOG	1	0.900	-	-		E1_COG3569
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2467
Inparanoid	1	1.050	530	1.000	Inparanoid score	I281
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D303947at2759
OrthoFinder	1	1.000	-	-		FOG0001157
OrthoInspector	1	1.000	-	-		otm2698
orthoMCL	1	0.900	-	-		OOG6_101409
Panther	1	1.100	-	-	O	PTHR10290
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X783
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.830