back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top1 and TOP1

DIOPT Version :9

Sequence 1:	NP_001014742.1	Gene:	Top1 / 32458	FlyBaseID:	FBgn0004924	Length:	974	Species:	Drosophila melanogaster
Sequence 2:	NP_003277.1	Gene:	TOP1 / 7150	HGNCID:	11986	Length:	765	Species:	Homo sapiens

Alignment Length:	963	Identity:	474/963 - (49%)
Similarity:	576/963 - (59%)	Gaps:	228/963 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 HHHSSSS-------SSSSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSK 95
            |.|:.|.       :.|.||    ||||:|||..|         ||||   ::|||.||..|:|:
Human     5 HLHNDSQIEADFRLNDSHKH----KDKHKDREHRH---------KEHK---KEKDREKSKHSNSE 53

  Fly    96 HRDKDKE-RDGSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGSSSSSR 159
            |:|.:|: ::.....|:.|||..||||              ||  .:|||:|.::|.|.|..:..
Human    54 HKDSEKKHKEKEKTKHKDGSSEKHKDK--------------HK--DRDKEKRKEEKVRASGDAKI 102

  Fly   160 HKSSSSSRDKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDQPSYDGVFVKPEPVSQQLMHSG 224
            .|      :||..                     .||....||:|..||.||.|
Human   103 KK------EKENG---------------------FSSPPQIKDEPEDDGYFVPP----------- 129

  Fly   225 SVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNLSQASSCDYSMS 289
                                                 :|.|..:|:..:
Human   130 -------------------------------------KEDIKPLKRPRD---------------- 141

  Fly   290 QFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAMRKRKQEATDRPDGGMDND 354
                                         :||||
Human   142 -----------------------------EDDAD------------------------------- 146

  Fly   355 DDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGNV-KPKKK-KMKKEPEPAVSPGKRQK 417
                        .|.:|||.|..:|.|||..||..|  |.: |||.| |.||.|||     ..:|
Human   147 ------------YKPKKIKTEDTKKEKKRKLEEEED--GKLKKPKNKDKDKKVPEP-----DNKK 192

  Fly   418 AKAKVEEEEVWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAA 482
            .|.|.|||:.|:|||||:..:|:||..||||||||||.||.:|.||:||||||.::||.:.||.|
Human   193 KKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVA 257

  Fly   483 TFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKE 547
            ||:||||:|:|.|||:|..|||||:||.||..|:.||.:..||:|.:|..||:|::|.||..|||
Human   258 TFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKE 322

  Fly   548 EKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCG 612
            |||..|.|||.|:||:|||::|.|||:|.||::||||||||||.||||||:||||...|:.|||.
Human   323 EKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCS 387

  Fly   613 KDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDK 677
            ||:||||||||.:||||||||.||||.||.||:||.:|||||||||::|||||..||||||||.|
Human   388 KDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKK 452

  Fly   678 VIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDE-DQADTVGCCSLRVEHVQLHKE 741
            .:||||..||::||||||:||||||||||||||||||||||:| :.|||||||||||||:.||.|
Human   453 CVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPE 517

  Fly   742 LNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELME 806
            |:|:|.||.|||.|||||||||:|.|||||||||:||||:|:..|||||||||.:||:||::|||
Human   518 LDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLME 582

  Fly   807 GLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENL 871
            |||||||||||||.|||.||..||.|...:|.|:|:||||||||||||||||:.||:.||||.||
Human   583 GLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNL 647

  Fly   872 KEKIKAKREAIEKCESEYHS--------RD-------EKKGKQLERLRDQLKKLELQETDRDENK 921
            :.||.||:|.:.....:..|        :|       |.|.|.::||.:||.|||:|.|||:|||
Human   648 QTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENK 712

  Fly   922 TIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 974
            .|||||||||||||||:||||||..||||||:|||||.||.||:.||||:|.|
Human   713 QIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF 765

Known Domains:

Indicated by green bases in alignment.

Software error:

Illegal division by zero at /www/www.flyrnai.org/docroot/cgi-bin/DRSC_prot_align.pl line 591.

For help, please send mail to the webmaster (ritg@hms.harvard.edu), giving this error message and the time and date of the error.