DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top1 and top1b

DIOPT Version :9

Sequence 1:	NP_001014742.1	Gene:	Top1 / 32458	FlyBaseID:	FBgn0004924	Length:	974	Species:	Drosophila melanogaster
Sequence 2:	XP_002666448.2	Gene:	top1b / 327179	ZFINID:	ZDB-GENE-030131-5390	Length:	751	Species:	Danio rerio

Alignment Length:	964	Identity:	445/964 - (46%)
Similarity:	564/964 - (58%)	Gaps:	237/964 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    33 GHHHHHHHSSSSSSSSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSKHR 97
            |.|.|.|....|.|...:....||||:||:..|         ||.|
Zfish     3 GDHAHKHRQVESGSVEYNSHKHKDKHKDRQHGH---------KEQK------------------- 39

  Fly    98 DKDKERDGSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGSSSSSR--- 159
             :||||..|.|.|                |:|||||        :::.|:|:|||......|
Zfish    40 -RDKERVNSKNGH----------------SEHKSSS--------ERKHREKEKDRLKHKEKRKEE 79

  Fly   160 --HKSSSSSRDKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDQPSYDGVFVKPEPVSQQLMH 222
              |.|....||||..                                     ||.|.
Zfish    80 KFHGSDGKYRDKENG-------------------------------------FVSPS-------- 99

  Fly   223 SGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNLSQASSCDYS 287
                                                        .||.||::.|:
Zfish   100 --------------------------------------------SIKVEEDTDNH---------- 110

  Fly   288 MSQFRADEPPFVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAMRKRKQEATDRPDGGMD 352
                      ..||||:                            ||   ||::
Zfish   111 ----------HTVKHEK----------------------------PL---KRER----------- 123

  Fly   353 NDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDY-GNVKPKKKKMKKEPEPAVSPGKRQ 416
              |:|:.      :.|.:|:|.|:::|.|||.:||...|: ..|:.|.||..||.:...:.||:
Zfish   124 --DEDEY------EHKPKKVKVENEKKVKKRKQEEEDSDFEERVEKKPKKKIKEEKKDTTGGKK- 179

  Fly   417 KAKAKVEEEEVWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEA 481
              |||.:.||.|:|||||:..||.||..||||||||.|.||.:|.:::|||||..::||...||.
Zfish   180 --KAKNQPEEKWKWWEEERYTDGSKWRFLEHKGPVFPPPYEPLPGHIKFYYDGNAMKLSPAAEEV 242

  Fly   482 ATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASK 546
            ||||||||.|:|.|.|:|..|||||:||.||..|:..|.:.:||:|.:|..||:|:||.||..||
Zfish   243 ATFYAKMLEHEYTTNEIFRKNFFKDWRKEMTSEEKSKITELKKCDFTQMHEYFKAQSEARKQMSK 307

  Fly   547 EEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINC 611
            |||...|.|||.:::|:|||::|.|:|:|||||:||||||||||:|||||.:||||:..|:.|||
Zfish   308 EEKQKNKEENERILQEYGFCLMDNHRERIGNFRIEPPGLFRGRGDHPKMGKLKRRIRPEDIIINC 372

  Fly   612 GKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLD 676
            .|||..|..|||:|||||||||.||||.||.||:||.:|||||||||::|||||..||||||||.
Zfish   373 SKDSVQPKAPPGTRWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLK 437

  Fly   677 KVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDE-DQADTVGCCSLRVEHVQLHK 740
            |.:::|||.||::|:|||||:||||||||||||||||||||||| :.|||||||||||||::||.
Zfish   438 KCVERIRAQYREDWRSKEMRIRQRAVALYFIDKLALRAGNEKDEGETADTVGCCSLRVEHIKLHP 502

  Fly   741 ELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELM 805
            :|:|::.||.|||.|||||||||::.|||||||||:||:|:|:..|||||||||.:||::|:|||
Zfish   503 QLDGQDYVVEFDFLGKDSIRYYNKIPVEKRVFKNLQLFLENKQSEDDLFDRLNTSILNKYLQELM 567

  Fly   806 EGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMEN 870
            :|||||||||||||.|||.||:.||.|...:|||:|:|||||||||:||||||:.||:.||||:|
Zfish   568 DGLTAKVFRTYNASITLQQQLNELTSPGENIPEKILSYNRANRAVALLCNHQRAPPKTFEKSMQN 632

  Fly   871 LKEKIKAKREAIEKCESEYHS--------RDEK-------KGKQLERLRDQLKKLELQETDRDEN 920
            |:.||..|::.:...:.|:.:        .|||       |.|.::|:.:||.|||:|.|||:||
Zfish   633 LQTKIDEKKKQLSAAKKEHKAAKADAKALHDEKSKKAVETKKKAVQRISEQLMKLEVQATDREEN 697

  Fly   921 KTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 974
            |.|||||||||||||||||:||||..||||||::||||.||.||:.||||::.|
Zfish   698 KQIALGTSKLNYLDPRISVSWCKKWGVPIEKIYSKTQRDKFAWAIDMADEDFEF 751

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top1	NP_001014742.1	Topoisomer_IB_N_htopoI_like	441..655	CDD:239570	132/213 (62%)
		TOPEUc	585..946	CDD:214661	252/376 (67%)
		Topoisom_I	657..859	CDD:279380	148/202 (73%)
		Topo_C_assoc	905..972	CDD:291068	51/66 (77%)
top1b	XP_002666448.2	Topoisomer_IB_N_htopoI_like	202..416	CDD:239570	132/213 (62%)
		TOPEUc	346..723	CDD:214661	252/376 (67%)
		Topoisom_I	418..621	CDD:279380	148/202 (73%)
		Topo_C_assoc	682..749	CDD:291068	51/66 (77%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	334	1.000	Domainoid score	I1104
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	827	1.000	Inparanoid score	I445
OMA	1	1.010	-	-		QHG62324
OrthoDB	1	1.010	-	-		D208288at33208
OrthoFinder	1	1.000	-	-		FOG0001157
OrthoInspector	1	1.000	-	-		otm24282
orthoMCL	1	0.900	-	-		OOG6_101409
Panther	1	1.100	-	-	O	PTHR10290
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2314
SonicParanoid	1	1.000	-	-		X783
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	13.010