DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top1 and top-1

DIOPT Version :9

Sequence 1:	NP_001014742.1	Gene:	Top1 / 32458	FlyBaseID:	FBgn0004924	Length:	974	Species:	Drosophila melanogaster
Sequence 2:	NP_493337.1	Gene:	top-1 / 266847	WormBaseID:	WBGene00006595	Length:	806	Species:	Caenorhabditis elegans

Alignment Length:	967	Identity:	433/967 - (44%)
Similarity:	565/967 - (58%)	Gaps:	196/967 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 HHHSSSSSSSSKHKSSSKDKHRDREREHKSSNSSSSSKEHKSSSRDKDRHKSSSSSSKHRDKDKE 102
            :|||:.:.:|:.:.::..|   :.::|.|....:|.|:........:|         |...|.|:
Worm     6 NHHSNGNGNSTVYDTNGND---EIKKEVKDEPMASDSEVPFGELMKRD---------KKEKKQKK 58

  Fly   103 RDGSSNSHRSGSSSSHKDKDGSSSSKHKSSSGHHKRSSKDKERRDKDKDRGSSSSSRHKSSSSSR 167
            |...|.|          |:|.....|.|||:           :..|.||.||.|...:|.
Worm    59 RKAESGS----------DEDDYKPEKRKSSA-----------KNGKKKDVGSDSEDDYKP----- 97

  Fly   168 DKERSSSSHKSSSSSSSSKSKHSSSRHSSSSSSKDQPSYDGVFVKPEPVSQQLMHSGSVDAFQMQ 232
            :|::|..::|        |....||......|..|....|   |||:      :||
Worm    98 EKKKSKKNNK--------KKAQESSEDDDEESEGDVSEED---VKPQ------IHS--------- 136

  Fly   233 QLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEEESFNNLSQASSCDYSMSQFRADEPP 297
                                              |.:.|||                    ||.|
Worm   137 ----------------------------------DDELEEE--------------------DEAP 147

  Fly   298 FVVKHEQSYAEEDSTMNYNDHDDDADEMNDDEEDVPLAMRKRKQEATDRPDGGMDNDDDDDIPLL 362
                                       ..||||:     :|||::...:.:
Worm   148 ---------------------------TTDDEEE-----QKRKEKERRKKE-------------- 166

  Fly   363 ARKKVKKEKIKKESKEKSKKRVKE---EPSDDYGNVKPKKKKMK----KEPEPAVSP----GKRQ 416
              |:.|||:.:|:..||..:::||   |.|||..:.|.||||.|    ::.:|:.|.    ||::
Worm   167 --KREKKERKEKKRLEKENRKIKEEDDEDSDDEDDEKAKKKKRKSKGAEKSKPSTSKKDAGGKKE 229

  Fly   417 KAKAKVEEEE----VWRWWEEEKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEE 477
            ..|.||::||    :|.||:|||:..||||::|:|.||:|||.|..:|.:|.|.|.|:.::|:.|
Worm   230 PPKKKVKKEEDIEDIWEWWKEEKKPAGVKWNSLQHCGPLFAPPYIPLPSHVHFKYGGEKMKLTLE 294

  Fly   478 TEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRK 542
            |||.|.|||.:|:|:|.|||.||.||.||:||.||..|||.|.|.:||:|:.:..|.:.:.|.||
Worm   295 TEEIAQFYAGVLDHEYSTKEAFNKNFMKDWRKVMTVEERERIHDLKKCDFRAIDAYQKEQREIRK 359

  Fly   543 AASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDV 607
            |.:|||||..|.|.||.:|.:|..:||||::|:.|||:||||:|||||.|||||:||:||...||
Worm   360 AMTKEEKLKIKEEKEAEVKIYGIAIIDGHRQKVANFRIEPPGVFRGRGGHPKMGLIKKRIMPEDV 424

  Fly   608 SINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETA 672
            .||||||:::|.||||.:|||||||||||||.||.|:|.||.||||||||||:|||||..|||||
Worm   425 IINCGKDTEIPKPPPGHKWKEVRHDNTVTWLCSWTESVLGQNKYIMLNPSSKIKGEKDFEKYETA 489

  Fly   673 RRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQ-ADTVGCCSLRVEHV 736
            |||.|.|..||..|.|::|||||||||||.|||||||||||||||||.|: |||||||||||||:
Worm   490 RRLKKKIGGIRERYTDDFKSKEMRVRQRATALYFIDKLALRAGNEKDVDEAADTVGCCSLRVEHI 554

  Fly   737 QL--HKELN-----GKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNT 794
            :|  ..:||     .||.||.|||.|||||||:|.|.|||||:|||::|||.|...|||||||:|
Worm   555 KLFDSAKLNEDDKKEKEFVVEFDFLGKDSIRYFNRVSVEKRVYKNLKIFMEGKAPSDDLFDRLDT 619

  Fly   795 QVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRS 859
            ..||:||:.||:|||.|||||||||.|||.||..||:|...|..|:|:||||||.|||||||||:
Worm   620 ATLNDHLRSLMDGLTVKVFRTYNASITLQEQLIKLTNPKDNVAAKILSYNRANRQVAILCNHQRA 684

  Fly   860 VPKSHEKSMENLKEKIKAKREAIEKCESEYHS----RDEKKGKQLERLRDQLKKLELQETDRDEN 920
            |.|..::||:.|::|||.|::.:::.|:...|    ..||..|:.:||::|||||::..||:|||
Worm   685 VSKGFDESMQKLEQKIKDKKKEVKEAEAALKSARGAEKEKAQKKYDRLKEQLKKLKISRTDKDEN 749

  Fly   921 KTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHM---ADENYRF 974
            |.||||||||||:||||:||||||.:||:||:|.||.|.||.||:.|   :||.|.|
Worm   750 KQIALGTSKLNYIDPRITVAWCKKFEVPLEKVFTKTHREKFRWAIDMTNSSDEEYVF 806

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top1	NP_001014742.1	Topoisomer_IB_N_htopoI_like	441..655	CDD:239570	126/213 (59%)
		TOPEUc	585..946	CDD:214661	247/372 (66%)
		Topoisom_I	657..859	CDD:279380	146/209 (70%)
		Topo_C_assoc	905..972	CDD:291068	46/69 (67%)
top-1	NP_493337.1	Topoisomer_IB_N_htopoI_like	258..472	CDD:239570	126/213 (59%)
		TOPEUc	402..775	CDD:214661	247/372 (66%)
		Topoisom_I	474..684	CDD:279380	146/209 (70%)
		Topo_C_assoc	733..795	CDD:291068	43/61 (70%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160166797
Domainoid	1	1.000	296	1.000	Domainoid score	I799
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2467
Inparanoid	1	1.050	740	1.000	Inparanoid score	I302
Isobase	1	0.950	-	0	Normalized mean entropy	S549
OMA	1	1.010	-	-		QHG62324
OrthoDB	1	1.010	-	-		D208288at33208
OrthoFinder	1	1.000	-	-		FOG0001157
OrthoInspector	1	1.000	-	-		oto18122
orthoMCL	1	0.900	-	-		OOG6_101409
Panther	1	1.100	-	-	LDO	PTHR10290
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2314
SonicParanoid	1	1.000	-	-		X783
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	16	15.890