DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top1 and AgaP_AGAP004078

DIOPT Version :9

Sequence 1:	NP_001014742.1	Gene:	Top1 / 32458	FlyBaseID:	FBgn0004924	Length:	974	Species:	Drosophila melanogaster
Sequence 2:	XP_309629.5	Gene:	AgaP_AGAP004078 / 1270877	VectorBaseID:	AGAP004078	Length:	1088	Species:	Anopheles gambiae

Alignment Length:	1133	Identity:	617/1133 - (54%)
Similarity:	733/1133 - (64%)	Gaps:	204/1133 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSGDVAA---ENSIHIQNGGSCEVVQSNGVTTNGH----GHHHHHHHSSSSSSSSKH------KS 52
            ||.|.|.   .:|..:.||   .:.:.||| :|||    |.:...|.|..|||..||      |:
Mosquito     1 MSVDAAVPQPADSSKVMNG---MMERPNGV-SNGHSGGGGENGDRHKSHKSSSKDKHRDKDRDKN 61

  Fly    53 SSKDKHRDRERE---------HKSSNSSSSSKEHKSSSRDK---DRHKSSSSSSKHRDKDKERDG 105
            ..||||||::|:         ||||:.|||||...|||:||   |:.|.|||||.::||:|....
Mosquito    62 RDKDKHRDKDRDREKDKNRDKHKSSSHSSSSKSGSSSSKDKEKSDKEKHSSSSSSNKDKEKSHHS 126

  Fly   106 SSNSHRSGSSSSHKDKDGS-SSSKHKS-SSGHHKRSSKDKE------RRDKDKDRGSSSSSRHKS 162
            ||::|:|..|...:.||.. ...||:| ..|..:...:||:      |.|||||:..|||    |
Mosquito   127 SSSTHKSSKSDKDRSKDKERDKDKHRSREDGKDRDRDRDKKDRDKSSRSDKDKDKHKSSS----S 187

  Fly   163 SSSSRDKERSSSSHKSSSSSSS-----SKSKHSSSR-----HSSSSSS-------KDQPSYDG-V 209
            |||::||:|  ..|||||||||     .|:||||||     |||||||       :|:....| |
Mosquito   188 SSSNKDKDR--DKHKSSSSSSSRDKDKDKNKHSSSRRDKDKHSSSSSSSKHKDRHRDEKVASGEV 250

  Fly   210 FVK--PEPVSQQLMHSGSVDAFQMQQLGSYEAAAAGTNFNGNGNVAGANYKNGYEESIVDIKKEE 272
            .||  ||||..:.:                         ||.|.:...|          |...|.
Mosquito   251 KVKEEPEPVPTEFL-------------------------NGEGYIPQQN----------DAMVEN 280

  Fly   273 ESFN-----NLSQASSCDYSMSQFRADEPPFVVKHEQSYAEEDSTM--NYNDHD----DDADEMN 326
            .:..     ::|||||||||||||||||.||.:|..::..:..|..  :|.::|    |||...|
Mosquito   281 HAGPVGGKLDISQASSCDYSMSQFRADETPFALKQNETMDQTTSNQGDDYQNNDDEEGDDASRYN 345

  Fly   327 D------------------------------------DEEDVPLAMRKRKQEATDRPDGGMDNDD 355
            |                                    .:.:|..|::|...:..|.|..|.:.::
Mosquito   346 DFNTENGHHHEQQPHMYQQQQHQQQGYFGGMQQQHEQYQANVQAAIKKEPLDIKDEPQSGQEGEE 410

  Fly   356 --DDDIPLLARKK----------VKKEKIKKESKE-KSKKRVKE-----EPSDDYGNVKPKKKKM 402
              :|||||..|||          .:|:|||.|.|| |:|||..:     |..||..:.|.|:||.
Mosquito   411 ESEDDIPLSKRKKREAEEGYDEDGRKKKIKTEKKEKKNKKRSHDSDDANENDDDVNSSKKKQKKT 475

  Fly   403 KKEPEPAVSP-----------------------------GKRQKAKAKVEEE--EVWRWWEEEKR 436
            |.:.||.|.|                             ||..:|:.|.|||  |||:|||||||
Mosquito   476 KIKKEPGVEPPTQQAKKNAKAAPPMPGTNQARTGDQAGTGKTGRARVKKEEEEQEVWKWWEEEKR 540

  Fly   437 ADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNN 501
            .|||||..||||||||||.|:.:|.:|:|.||||.::||::.||.|.|||:||.|||.:|:.||:
Mosquito   541 DDGVKWKYLEHKGPVFAPPYDPLPSHVKFMYDGKEMKLSQDAEEVAGFYARMLEHDYTSKQAFND 605

  Fly   502 NFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFC 566
            |||||:||:|||.|||.|.|..||||:.|..||...:|:.:..|||||...|..||.|.||:|.|
Mosquito   606 NFFKDWRKTMTPHERERITDLEKCNFRPMAAYFAEIAEQNRNRSKEEKAALKAANEQLTKEYGIC 670

  Fly   567 MIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRH 631
            :|||||||||||::||||||||||||||||::|||:...||.|||.:|||:||||||.|||||||
Mosquito   671 VIDGHKEKIGNFKIEPPGLFRGRGEHPKMGLVKRRVMPEDVIINCSQDSKIPSPPPGRRWKEVRH 735

  Fly   632 DNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMR 696
            ||||:|||||:|||||||||||||||||||||||..||||||||.|.|||||.||||||||||||
Mosquito   736 DNTVSWLASWVENVQGQVKYIMLNPSSKLKGEKDWQKYETARRLLKHIDKIRDTYRDEWKSKEMR 800

  Fly   697 VRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRY 761
            :||||||:|||||||||||||||:||||||||||||.||:.||||||||||||||||.|||||||
Mosquito   801 IRQRAVAMYFIDKLALRAGNEKDDDQADTVGCCSLRYEHIALHKELNGKENVVVFDFLGKDSIRY 865

  Fly   762 YNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQL 826
            |||||||||||||||||.|:||.||||||||||.|:||:||.||:|||||||||:|||.|||.||
Mosquito   866 YNEVEVEKRVFKNLELFKENKKPGDDLFDRLNTSVVNEYLKGLMDGLTAKVFRTFNASYTLQKQL 930

  Fly   827 DLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEY-- 889
            :.||||:.:|||||||||||||||||||||||:|||:|||||.||||||:.|||.:|:|:.|:
Mosquito   931 EELTDPNMSVPEKLLAYNRANRAVAILCNHQRAVPKTHEKSMGNLKEKIRVKREQVEQCQKEFKD 995

  Fly   890 ----HSRD----EKKGKQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHD 946
                :.||    :||.||||||::|||||||||||||||||||||||||||||||||||||||..
Mosquito   996 LKKSNVRDPSAFDKKSKQLERLKEQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKFG 1060

  Fly   947 VPIEKIFNKTQRTKFLWAVHMADENYRF 974
            ||||||||||||.||.||:.||||||.|
Mosquito  1061 VPIEKIFNKTQRDKFRWAIDMADENYVF 1088

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top1	NP_001014742.1	Topoisomer_IB_N_htopoI_like	441..655	CDD:239570	143/213 (67%)
		TOPEUc	585..946	CDD:214661	302/370 (82%)
		Topoisom_I	657..859	CDD:279380	173/201 (86%)
		Topo_C_assoc	905..972	CDD:291068	59/66 (89%)
AgaP_AGAP004078	XP_309629.5	Topoisom_I_N	544..758	CDD:280987	143/213 (67%)
		TOPEUc	689..1060	CDD:214661	302/370 (82%)
		Topoisom_I	761..963	CDD:279380	173/201 (86%)
		Topo_C_assoc	1018..1086	CDD:291068	59/67 (88%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	388	1.000	Domainoid score	I2301
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H2467
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG62324
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001157
OrthoInspector	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR10290
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X783
TreeFam	0	0.000	Not matched by this tool.
	7	7.020