Protein Alignment Rab3-GEF and Madd

DIOPT Version :9

• Sequence 1: NP_001259560.1
• Gene: Rab3-GEF / 32442
• FlyBaseID: FBgn0030613
• Length: 2154
• Species: Drosophila melanogaster
• Sequence 2: XP_038961933.1
• Gene: Madd / 94193
• RGDID: 619922
• Length: 1647
• Species: Rattus norvegicus
• Alignment Length: 1930
• Identity: 748/1930 - (38%)
• Similarity: 1010/1930 - (52%)
• Gaps: 433/1930 - (22%)

  Fly     4 QQKASLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQ 68
            |:|  .||||:||:.||||.  .|....:       .|.|:||||||..||.:||||.|:|:|||
  Rat     3 QKK--FCPRLLDYLVIVGAR--HPSSDSV-------AQTPELLRRYPLEDHPEFPLPPDVVFFCQ 56

  Fly    69 PEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIER--PSSAAGSAGAGNDRP 131
            ||||.||..||  .::||.|||||.|||||:|.|||||||||||..::  |...
  Rat    57 PEGCLSVRQRR--MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEK----------- 108

  Fly   132 GNGGPGGHGGGAGGGAGGGGRGGRRSSAFRRESWRKSMERSSDSAFSSDYRSNVAPSDSDRELTS 196
                       |.||||..|:.|..:.....|:..:|.|..|.
  Rat   109 -----------AEGGAGPRGKEGAHAPCASEEAATESSESGST---------------------- 140

  Fly   197 RRDSDQQRLHSHHSHHQPHHPSASPAVPKLGLMAPSADSESGGSHSP-SPRASRKRTKLRNQSLT 260
                                           |..|||||....:.|| ..|.::...:.||.:||
  Rat   141 -------------------------------LQPPSADSTPDVNQSPRGKRRAKAGNRSRNSTLT 174

  Fly   261 SLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGH-VSDATPSIV 324
            |||::||:|||:||||||:.||:|:|.|:|....|:.|..:.:.||.:|.:.||. :.:...|.:
  Rat   175 SLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKPGIPRGVQRDTMWRIFTGSLLVEEKSSAL 239

  Fly   325 LHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLG 389
            |||:||||.||.|||.:||||.|..||::|||...|.:.|.|||||.:||:||||||||||||||
  Rat   240 LHDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQEVQQALTFALPDPSRFTLVDFPLHLPLELLG 304

  Fly   390 VETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAP 454
            |:.||:|.|.|:.|:||:.||||||||||||||||.|:||||||||||||||||::.||||||||
  Rat   305 VDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAP 369

  Fly   455 TPFVIGVPASFLVYKKNFRLPDDIWVVDLDSTKLTPPTGGYEEIPPLPEPEGTILKNHLKQAMLL 519
            ||::|||||||.:||.:|::|||:|:|||||.::..||.. |.:|.|||||...||.||||
  Rat   370 TPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNA-EVLPILPEPESLELKKHLKQ---- 429

  Fly   520 MDEAGLGALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQ 584
                   ||.||:.....:.:   |....:..:.|.|||                   |.|...|
  Rat   430 -------ALASMSLNTQPILN---LEKFHEGQETPLLLG-------------------RFSNDLQ 465

  Fly   585 GTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVA 649
            .|.|:.         .||.:||.||||||||||||||||||:.|.|..|..|||||||:||||||
  Rat   466 STPSTE---------FNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVA 521

  Fly   650 FQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHAL 714
            ||..|||.||||.:.|..:.|||||||:..||.|.|:|.||.|:.....||..:||||||:||.|
  Rat   522 FQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNYAFQRIHNNTFDPALIGDKPKWYAHQL 586

  Fly   715 TPIRFSVWDDGSSLNGALR--SLKQLECQPTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSP 777
            .||.:.|:|..|.|..||.  ..:..|..|||:|||||...|.||||||||.||||||...|::.
  Rat   587 QPIHYRVYDSNSQLAEALSVPPERDSESDPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDING 651

  Fly   778 SLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYPEGIADAVASKEEEDEERADS------- 835
                     :.|:|.|.|.::..|.:            |...|.:..::|.:|...||
  Rat   652 ---------DTPNVDPLTHAALGDAS------------EVEIDELQPQKEGEEPGPDSENSQENL 695

  Fly   836 PVSSSSSRSDLSSPSFNRDSEFDFQPKGGQTLGSTTVGSGAAAPSFELATPLAMRLEATIKMASI 900
            |:.||||.:..||||                    |:..||.:
  Rat   696 PLRSSSSTTASSSPS--------------------TIVHGAHS---------------------- 718

  Fly   901 EQESDTVSTATGKTIAAGSKLQRHPSDSESRPEKKIPPPLTPPVKQPGVSNILARTGSSGSSSSS 965
             :.:|  ||..|...|.|.          |:|    .||:.|.:.:..|......||     ..|
  Rat   719 -EPAD--STEVGDKAATGI----------SKP----LPPVPPSICKSTVDRRQTETG-----EGS 761

  Fly   966 PGRQSSQSSLFE-NFASHAKELVRETTRQSSQEGLLAHVDKFTLHAKKAAGEASKQALEVSKQAA 1029
            ..:::.....|| .:.|.|:|       ...::| .::..:|:.|   |:|..:::.|
  Rat   762 VCQRTYDHPYFEPQYGSPAEE-------DDDEQG-ESYTPRFSQH---ASGSRAQKLL------- 808

  Fly  1030 GVSKNTLEDLTYVGKSTLGDLTKTAKEAATKKGIIKIEEHSAGGAGPPPKSPGSQLATHKQVQQS 1094
                                          :...:|:...|...:.....||.|.::.:    .:
  Rat   809 ------------------------------RPNSLKLASDSDAESDSRASSPNSTVSNN----ST 839

  Fly  1095 GGQGGGNNFFSAIGTDFNGLASSTSTMFS----GMFGKKSQQKQVPVQQKQPNVSAGKAKSGIN- 1154
            .|.||..:|.|:       |..:.||.||    .:..|.:::|..|.    |::..    .|:|
  Rat   840 EGFGGIMSFASS-------LYRNHSTSFSLSNLTLPTKGAREKTTPF----PSLKV----FGLNT 889

  Fly  1155 -----FDPFPG-----RKGLV-ERTPLIKHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQ 1208
                 .:..||     |:.|| :::.:|||| |...:|.   ...|.||.:::|||.|||:|.:.
  Rat   890 LMEIVTEAGPGSGEGNRRALVDQKSSVIKHS-PTVKREP---PSPQGRSSNSSENQQFLKEVVHS 950

  Fly  1209 VLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKK-IAPDDHIDDVCVTKPVWKGMLKCIQ 1272
            ||.|:|||||.:.:.::|:|.|..|..|||||::.:..: .|..|.|.||.:::.|:||||..::
  Rat   951 VLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLSRAVQSEDDARQDVIQDVEISRKVYKGMLDLLK 1015

  Fly  1273 AIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKEINEGSDMSSSLLSSHAASPMGSRENL--- 1334
            .....|:.::|:.|||||||:|.|:|:|.|||:|||    .|......:.:..:|:|....|
  Rat  1016 CTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKE----PDKRKRSPTENVNTPVGKDPGLAGR 1076

  Fly  1335 ----------------RSPSSPNGSHSALGSE-------------WASPQESRKSST-------- 1362
                            |:||:.......|.:.             |...||.:|...
  Rat  1077 GDPKAMAQLRVPQLGPRAPSATGRGPKELDTRSLKEENFVASVELWNKHQEVKKQKALEKQRPEV 1141

  Fly  1363 -----QLAHGGPGGSHSGAPINRRLSSA------DSQDGQSTTEMFKDMLSQKRSALKNMLTSFD 1416
                 .|.......|...|.....|:||      ||..|.|...|.:   |..:.:..:.:.|..
  Rat  1142 IKPVFDLGETEEKKSQISADSGVSLASASQRTDQDSVIGVSPAVMIR---SSSQDSEVSTVVSNS 1203

  Fly  1417 SDTTTSKDSKKS-----------SGNLWSGKSTLSAGFRYTG---------GHLINTSSSPSPD- 1460
            |..|...||..|           |.:|.|.::|||.....|.         .|.:.....|:..
  Rat  1204 SGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSTIFGKAHSLKPKEKPASSP 1268

  Fly  1461 -------SPRVYLFEGLLGKD---------------------RLNLWNQMQFWEDAFLDAVSQER 1497
                   |.||||:|||||:|                     |..||:|||||||||||||..||
  Rat  1269 VRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQMQFWEDAFLDAVMLER 1333

  Fly  1498 DMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLG 1562
            :.:||||||.||::||.||.|.:|||||.||||||:|:|:||.:.::::.|.|::||:|:|||:|
  Rat  1334 EGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMG 1398

  Fly  1563 KSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLV 1627
            |||:||||||:::.|:||:.||||.|:.::..|||.:.:|:|.||.|||.||.:.||||.||.:|
  Rat  1399 KSHVGLVYSQQINEVLDQLTNLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVV 1463

  Fly  1628 LRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGT 1692
            |||..||:.||||||:|:||||.||||:||||||||.:|||:|:||:||:|||.|:|::|||...
  Rat  1464 LRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAA 1528

  Fly  1693 PTNV----PELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNS---KFFVRLDHIRKCFTQKGGIF 1750
            ....    ||||||||||||.|||||||||.|||:.|.|.::   |.|:.|:||:||.|.: |:|
  Rat  1529 RQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHNQERKVFIELNHIKKCNTVR-GVF 1592

  Fly  1751 VLEEYNPKTRNLIQRKYQSSMSDQICYSVLCVFSYVAAGQDQKKN 1795
            ||||:.|:.:.::..||::.|:.:|||||||:||||||.:..:::
  Rat  1593 VLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAAVRSSEED 1637

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rab3-GEF	NP_001259560.1	uDENN	10..111	CDD:214824	59/100 (59%)
		DENN	260..486	CDD:214823	144/226 (64%)
		dDENN	613..681	CDD:129037	46/67 (69%)
Madd	XP_038961933.1	uDENN	7..97	CDD:214824	59/100 (59%)
		DENN	172..401	CDD:214823	145/228 (64%)
		dDENN	485..553	CDD:129037	46/67 (69%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353853
Domainoid	1	1.000	763	1.000	Domainoid score	I81
eggNOG	1	0.900	-	-		E1_KOG3570
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H14249
Inparanoid	1	1.050	1175	1.000	Inparanoid score	I188
OMA	1	1.010	-	-		QHG48534
OrthoDB	1	1.010	-	-		D162625at2759
OrthoFinder	1	1.000	-	-		FOG0006143
OrthoInspector	1	1.000	-	-		oto97185
orthoMCL	1	0.900	-	-		OOG6_106194
Panther	1	1.100	-	-	LDO	PTHR13008
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4435
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	13	12.900