DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rab3-GEF and MADD

DIOPT Version :9

Sequence 1:	NP_001259560.1	Gene:	Rab3-GEF / 32442	FlyBaseID:	FBgn0030613	Length:	2154	Species:	Drosophila melanogaster
Sequence 2:	NP_001363500.1	Gene:	MADD / 8567	HGNCID:	6766	Length:	1650	Species:	Homo sapiens

Alignment Length:	1938	Identity:	752/1938 - (38%)
Similarity:	985/1938 - (50%)	Gaps:	461/1938 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 QKASLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQP 69
            ||...||||:||:.||||.  .|....:       .|.|:||||||..||.:||||.|:|:||||
Human     3 QKKKFCPRLLDYLVIVGAR--HPSSDSV-------AQTPELLRRYPLEDHTEFPLPPDVVFFCQP 58

  Fly    70 EGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIE-RPSSAAGSAGAGNDRPGN 133
            |||.||..||  .::||.|||||.|||||:|.|||||||||||..: |.|...|.
Human    59 EGCLSVRQRR--MSLRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRISKEKGE---------- 111

  Fly   134 GGPGGHGGGAGGGAGGGGRGGRRSSAFRRESWRKSMERSSDSAFSSDYRSNVAPSDSDRELTSRR 198
                       ||||..|:.|..::....|...:|.|..|
Human   112 -----------GGAGSRGKEGTHATCASEEGGTESSESGS------------------------- 140

  Fly   199 DSDQQRLHSHHSHHQPHHPSASPAVPKLGLMAPSADSESGGSHSP-SPRASRKRTKLRNQSLTSL 262
                                        .|...||||....:.|| ..|.::..::.||.:||||
Human   141 ----------------------------SLQPLSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSL 177

  Fly   263 CIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGH-VSDATPSIVLH 326
            |::||:|||:||||||:.||:|:|.|:|....|::|..:.:.||.:|.:.||. :.:...|.:||
Human   178 CVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEKSSALLH 242

  Fly   327 DVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGVE 391
            |:||||.||.|||.:||||.|..||::|||...:...|.|||||.:||:|||||||||||||||:
Human   243 DLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQPALTFALPDPSRFTLVDFPLHLPLELLGVD 307

  Fly   392 TCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPTP 456
            .||:|.|.|:.|:||:.||||||||||||||||.|:||||||||||||||||::.||||||||||
Human   308 ACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAPTP 372

  Fly   457 FVIGVPASFLVYKKNFRLPDDIWVVDLDSTKLTPPTGGYEEIPPLPEPEGTILKNHLKQAMLLMD 521
            ::|||||||.:||.:|::|||:|:|||||.::..||.. |.:|.|||||...||.||||
Human   373 YIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNA-EVLPILPEPESLELKKHLKQ------ 430

  Fly   522 EAGLGALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQGT 586
                 ||.||:.....:.:   |....:..:.|.|||                   |.|...|.|
Human   431 -----ALASMSLNTQPILN---LEKFHEGQEIPLLLG-------------------RPSNDLQST 468

  Fly   587 ISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAFQ 651
            .|:.         .||.:||.||||||||||||||||||:.|.|..|..|||||||:||||||||
Human   469 PSTE---------FNPLIYGNDVDSVDVATRVAMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQ 524

  Fly   652 INSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALTP 716
            ..|||.||||.:.|..:.|||||||:..||.|.|||.||.|:...:.||..:||||||:||.|.|
Human   525 AGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNMFDPALIGDKPKWYAHQLQP 589

  Fly   717 IRFSVWDDGSSLNGALR--SLKQLECQPTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPSL 779
            |.:.|:|..|.|..||.  ..:..:.:|||:|||||...|.||||||||.||||||...|::.
Human   590 IHYRVYDSNSQLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYSSLGDFVSEMMKCDING-- 652

  Fly   780 HDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYPEGIADAVASKEEEDEERADS-------PV 837
                   :.|:|.|.|.::..|.:            |...|.:.:::|.:|...||       |:
Human   653 -------DTPNVDPLTHAALGDAS------------EVEIDELQNQKEAEEPGPDSENSQENPPL 698

  Fly   838 SSSSSRSDLSSPSFNRDSEFDFQPKGGQTLGSTTVGSGAAAPSFELATPLAMRLEATIKMASIEQ 902
            .||||.:..||||                    ||..||   :.|.|....|..:|.:   .:.:
Human   699 RSSSSTTASSSPS--------------------TVIHGA---NSEPADSTEMDDKAAV---GVSK 737

  Fly   903 ESDTVSTATGKT--------IAAGSKLQRHPSDSESRPEKKIPP---------PLTPPVKQPGVS 950
            ...:|..:.||:        |..||..:|...:....|:...||         ..||...|....
Human   738 PLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQHVSG 802

  Fly   951 N---ILARTGS----SGSSSSSPGRQSSQSSLFEN--------FASHAKELVRETTRQSSQEGLL 1000
            |   .|.|..|    |.|.:.|..|.||.:|...|        ..|.|..|.|.
Human   803 NRAQKLLRPNSLRLASDSDAESDSRASSPNSTVSNTSTEGFGGIMSFASSLYRN----------- 856

  Fly  1001 AHVDKFTLHAKKAAGEASKQALEVSKQAAGVSKNTLEDLTYVGKSTLGDLTKTAKEAATKKGIIK 1065
             |...|:|                       |..||.             ||.|:|.||
Human   857 -HSTSFSL-----------------------SNLTLP-------------TKGAREKAT------ 878

  Fly  1066 IEEHSAGGAGPPPKSPGSQLATHKQVQQSGGQGGGNNFFSAIGTDFNGLASSTSTMFSGMFGKKS 1130
                      |.|......|.|..::....|.|.|..
Human   879 ----------PFPSLKVFGLNTLMEIVTEAGPGSGEG---------------------------- 905

  Fly  1131 QQKQVPVQQKQPNVSAGKAKSGINFDPFPGRKGLVERTPLIKHSGPRQTQEELTRQQNQERSHSN 1195
             .::..|.||                     ..:::.:|.:|...|          ..|.||.::
Human   906 -NRRALVDQK---------------------SSVIKHSPTVKREPP----------SPQGRSSNS 938

  Fly  1196 AENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTLDKK-IAPDDHIDDVCV 1259
            :|||.|||:|.:.||.|:|||||.:.:.::|:|.|..|..||||||:.:..: .|..|.|.||.:
Human   939 SENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI 1003

  Fly  1260 TKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKEINEGSDMSSSLLSSHA 1324
            ::.|:||||..::.....|:.::|:.|||||||:|.|:|:|.|||:|||    .|......:...
Human  1004 SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKE----PDKRKRSPTESV 1064

  Fly  1325 ASPMGSRENL-------------------RSPSSPNGSHSALGSE-------------WASPQES 1357
            .:|:|....|                   |:||:.......|.:.             |...||.
Human  1065 NTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFIASIELWNKHQEV 1129

  Fly  1358 RKSST-------------QLAHGGPGGSHSGAPINRRLSSA------DSQDGQSTTEMFKDMLSQ 1403
            :|...             .|.......|...|.....|:|:      ||..|.|...|.:   |.
Human  1130 KKQKALEKQRPEVIKPVFDLGETEEKKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMIR---SS 1191

  Fly  1404 KRSALKNMLTSFDSDTTTSKDSKKSSG-----------NLWSGKSTLSAGFRYTG---------G 1448
            .:.:..:.:.|..|..|...||..||.           :|.|.:.|||.....|.         .
Human  1192 SQDSEVSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVHLASSRGTLSDSEIETNSATSTIFGKA 1256

  Fly  1449 HLINTS------SSP---SPD-SPRVYLFEGLLGKD---------------------RLNLWNQM 1482
            |.:..|      .||   |.| |.||||:|||||:|                     |..||:||
Human  1257 HSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSISKERSTLWDQM 1321

  Fly  1483 QFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILVMLN 1547
            |||||||||||..||:.:||||||.||::||.||.|.:|||||.||||||:|:|:||.:.::::.
Human  1322 QFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMK 1386

  Fly  1548 VAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQGTDV 1612
            |.|::||:|:|||:|||||||||||:::.|:||:.||||.|:.:...|||.:.:|:|.||.|||.
Human  1387 VNKNDIRKKVRRLMGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDT 1451

  Fly  1613 NGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTRKCK 1677
            ||.:.||||.||.:||||..||:.||||||:|:||||.||||:||||||||.:|||:|:||:||:
Human  1452 NGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCR 1516

  Fly  1678 ELYNCIKEAMERGGTPTNV----PELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNS---KFFVR 1735
            |||.|:|::|||.......    |||||||||||:.|||||||||.|||:.|.|.::   |.|:.
Human  1517 ELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQERKVFIE 1581

  Fly  1736 LDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMSDQICYSVLCVFSYVAA 1788
            |:||:||.|.: |:|||||:.|:.:.::..||::.|:.:|||||||:||||||
Human  1582 LNHIKKCNTVR-GVFVLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLFSYVAA 1633

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rab3-GEF	NP_001259560.1	uDENN	10..111	CDD:214824	59/100 (59%)
		DENN	260..486	CDD:214823	143/226 (63%)
		dDENN	613..681	CDD:129037	46/67 (69%)
MADD	NP_001363500.1	uDENN	8..98	CDD:214824	59/100 (59%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	108..168		19/133 (14%)
		DENN	173..402	CDD:214823	144/228 (63%)
		dDENN	486..554	CDD:129037	46/67 (69%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	604..636		15/31 (48%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	678..842		46/189 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	913..941		8/58 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1051..1110		10/62 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1146..1243		23/99 (23%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159790
Domainoid	1	1.000	743	1.000	Domainoid score	I96
eggNOG	1	0.900	-	-		E1_KOG3570
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H14249
Inparanoid	1	1.050	1158	1.000	Inparanoid score	I208
Isobase	1	0.950	-	0	Normalized mean entropy	S6066
OMA	1	1.010	-	-		QHG48534
OrthoDB	1	1.010	-	-		D162625at2759
OrthoFinder	1	1.000	-	-		FOG0006143
OrthoInspector	1	1.000	-	-		oto90067
orthoMCL	1	0.900	-	-		OOG6_106194
Panther	1	1.100	-	-	LDO	PTHR13008
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4941
SonicParanoid	1	1.000	-	-		X4435
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.840