Protein Alignment Rab3-GEF and madd

DIOPT Version :10

• Sequence 1: NP_001259560.1
• Gene: Rab3-GEF / 32442
• FlyBaseID: FBgn0030613
• Length: 2154
• Species: Drosophila melanogaster
• Sequence 2: XP_012816822.1
• Gene: madd / 780174
• XenbaseID: XB-GENE-5787338
• Length: 1662
• Species: Xenopus tropicalis
• Alignment Length: 1966
• Identity: 731/1966 - (37%)
• Similarity: 1005/1966 - (51%)
• Gaps: 500/1966 - (25%)

  Fly     1 MSDQQKASLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVY 65
            |..::||  ||||:||:.|:||.  .|....:.       |.|:||||||..||.:||||.|:|:
 Frog     1 MGQRKKA--CPRLLDYLVIIGAR--QPSSDSIS-------QTPELLRRYPLEDHPEFPLPPDVVF 54

  Fly    66 FCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIE------------RPS 118
            |||||||.||..:|  .::||..||||.|||||||..||||||||||..:            :|:
 Frog    55 FCQPEGCLSVRQKR--MSLRDDMSFVFTLTDKDSGVIRYGICVNFYRSFQKRLPKDKSRKPPKPA 117

  Fly   119 SAAGSAGAGNDRPGNGGPGGHGGGAGGGAGGGGRGGRRSSAFRRESWRKSMERSSDSAFSSDYRS 183
            |..||         .|..|||
 Frog   118 SENGS---------EGSDGGH-------------------------------------------- 129

  Fly   184 NVAPSDSDRELTSRRDSDQQRLHSHHSHHQPHHPSASPAVPKLGLMAPSADSESGGSHSP-SPRA 247
               |.                        |||...::|.:                :.|| ..|.
 Frog   130 ---PM------------------------QPHSAESTPDI----------------NQSPRGKRL 151

  Fly   248 SRKRTKLRNQSLTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVL 312
            |:...:.||.:|||||||||:|||:||||||:.||:|:|.|:|....|:.|.::.:.||.:|.:.
 Frog   152 SKGMQRSRNSTLTSLCIISHYPFFSTFRECLYTLKRLVDCCSERLVSKKAGVTKGVQRDTMWRIF 216

  Fly   313 TGHVS-DATPSIVLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHE-PLLFALPDHTRFS 375
            ||.:. :..|:::|||:||||.||.|||.:|.||||..||:|||| |..|: ||.|||||.:|||
 Frog   217 TGSLQLEEKPNVLLHDLREIEAWIYRLLRSPTPVPGQKRVDVEVL-PLDHQPPLTFALPDPSRFS 280

  Fly   376 LVDFPLHLPLELLGVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLL 440
            ||||||||||||||||.||:|.:.||.|:||:.||||||||||||||||:|:|||||||||||||
 Frog   281 LVDFPLHLPLELLGVEACLQVLSCIMLEHKVVMQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLL 345

  Fly   441 PTCLSCAEQLLLAPTPFVIGVPASFLVYKKNFRLPDDIWVVDLDSTKLTPPTGGYEEIPPLPEPE 505
            |||::.||||||||||::|||||||.:||.:|::|||:|:||||:.|:..||.. :.:|.|||||
 Frog   346 PTCMASAEQLLLAPTPYIIGVPASFFLYKSDFKMPDDVWLVDLDTNKVVAPTNA-DLLPVLPEPE 409

  Fly   506 GTILKNHLKQAMLLMDEAGLGALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPP 570
            .:.||.||||.        |.:::.:|......|..:.|.|:  ||...|:|.:.:    ....|
 Frog   410 ASELKKHLKQC--------LHSMSVITQQRLLASDNKALASM--SLNTQPILNLDR----FHEGP 460

  Fly   571 HSAQASQRNSMSAQGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAE 635
            .:|..  :....||||.|:.         .||.:||.||||||||||||||||||:.|.|.:|..
 Frog   461 DAALL--KAPSDAQGTPSTE---------FNPLIYGNDVDSVDVATRVAMVRFFNSPNMLHSFQM 514

  Fly   636 HTRTLRLYPRPVVAFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDP 700
            |||||||:||||||||.:|||.:||:.|.|..:.::||||||..||||.|||.||.|:...:.||
 Frog   515 HTRTLRLFPRPVVAFQSSSFLAARPKPSLFTEKLSKTQAVEFFGEWSLNPTNFAFQRIHNNMFDP 579

  Fly   701 MQVGDKPKWFAHALTPIRFSVWDDGSSLNGA--LRSLKQLECQPTDESGSDSEGADSSSSSYSSL 763
            ..:||||||:||.|.||.:.|:|..|.|..|  :.:.:..:..|||:|||||...|.||||||||
 Frog   580 ALIGDKPKWYAHQLQPIHYRVYDANSQLAEAFCVPAERDSDSDPTDDSGSDSVEYDDSSSSYSSL 644

  Fly   764 SDFVSEMASSDLSPSLHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYPEGIADAVASKEEE 828
            .||||||...|::.         :.|:|.|.|.::                   :.|| :..|.:
 Frog   645 GDFVSEMMKCDING---------DTPNVDPLTHAA-------------------LGDA-SEVEFD 680

  Fly   829 DEERADSPVSSSSSRSDLSSPSFNRDSEFDFQPKGGQTLGSTTVGS-------GAAAPSFELATP 886
            |.:.....:..::..|:        :|:.:.||:.|   .|||..|       ||...|.:.|.|
 Frog   681 DFQEYSGEIEENNGDSE--------NSQDNNQPRSG---SSTTASSSPSTVIHGANTESADSAEP 734

  Fly   887 -----------LAMRLEATIKMASIEQESDTVSTATGKTIAAGSKLQRHPSDSESRPEKKIPP-- 938
                       |.:......::|...:|||.       ::..|...:|...:....|:...||
 Frog   735 EEKVMGVLQNHLPVLPPTFTRLAMERRESDV-------SLGGGEARRREYDNPYFEPQYGFPPEE 792

  Fly   939 ------------------------PLTPPVKQPGVSNILARTGSSGSS-SSSPGRQSSQSSLFEN 978
                                    ||..|......|:..|.|.|.||| :|:....||.:..|..
 Frog   793 ENDEREESYTPMFNQSVNGTRSPKPLLRPNSLKLASDSDAETDSRGSSPTSTVSNNSSNNEGFGG 857

  Fly   979 FASHAKELVRETTRQSSQEGLLAHVDKFTLHAKKAAGEASKQALEVSKQAAGVSKNTLEDLTYVG 1043
            ..|.|..|.:            .|...|:|.:.....::..:|            .....|...|
 Frog   858 IMSFASNLYK------------THSSSFSLSSLTLPSKSRDKA------------TPFPSLKVFG 898

  Fly  1044 KSTLGDLTKTAKEAATKKGIIKIEEHSAGGAGPPPKSPGSQLATHKQVQQSGGQGGGNNFFSAIG 1108
            .:|:.::                    ...:||.|..                   |:|
 Frog   899 LNTIMEI--------------------IADSGPAPAE-------------------GSN------ 918

  Fly  1109 TDFNGLASSTSTMFSGMFGKKSQQKQVPVQQKQPNVSAGKAKSGINFDPFPGRKGLVERTPLIKH 1173
                                    ::..|.||    |:|                 ::.:|.:|.
 Frog   919 ------------------------RRALVDQK----SSG-----------------IKHSPTVKR 938

  Fly  1174 SGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLS 1238
            ..|          ..|.:|::::|||.|||:|...||.|:|||||.:.:.::|:|.|..|..|||
 Frog   939 ESP----------SPQGKSNNSSENQQFLKEVALGVLEGQGVGWLNMKKVRRLLESEQLRVFVLS 993

  Fly  1239 KLNKTLD-KKIAPDDHIDDVCVTKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHT 1302
            |||:::. ::.|.:|.|.:|.:|:.|::|||..::.....|:.|:||.|||||||||.|:|:|||
 Frog   994 KLNRSIQTEEDARNDVIQEVEITRKVYRGMLDLLKCTVSSLEQTYANAGLGGMASVFSLLEIAHT 1058

  Fly  1303 HYWSKEIN--------EGS-------DMSSSLLSSHAASPMGSRENLRSPSSPNG---------- 1342
            ||::||..        :|:       .:|:..:.|..|..:.:::.|..||.|.|
 Frog  1059 HYYNKEPEKRKHSPSADGAVTPVSRDPVSAPRVESRPAPQLPAKQLLPRPSGPAGRGAREFDTRS 1123

  Fly  1343 --------------SHSALGSEWASPQESRKSSTQLAHGGPGGSHSGAPINRRLSSADSQDGQST 1393
                          :|..:..:.|..::|.|.:..|...|        ....:.|...:..|.|.
 Frog  1124 LKEENFVASIELWNTHREVKKQKAMDKQSTKPAPPLVDSG--------ETEEKKSQISADSGLSV 1180

  Fly  1394 TEMFKDMLSQKRSALKN-------MLTSFDSDTTTSKDSKKSSGNLWSGKSTLSAGFRYT----- 1446
            |.      ..:||.|..       ::.|...|:..|.....|||......|.||:.....
 Frog  1181 TS------GSQRSDLDTGSIGPSILIRSSSQDSEISTVVSNSSGETLGADSDLSSNAGDALGGPG 1239

  Fly  1447 GGHLINTSSS-----------------------------------PSPD-SPRVYLFEGLLGKD- 1474
            |.||..:..:                                   |..| |.|:||:|||||:|
 Frog  1240 GSHLSGSRGAVSDSEIQMNSFTGAIFGKPQMLRPGEKERNVSPGRPQQDLSQRIYLYEGLLGRDK 1304

  Fly  1475 --------------------RLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSES 1519
                                |..||:|||||||||||||..||:.:||||||.||::||.||.:.
 Frog  1305 GSVWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYFSLGDH 1369

  Fly  1520 ERKRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNL 1584
            :|||||.||||||:|:|:|:.|.::|:.|.|.::|||:||:|||.|||||:||:::.::|::.::
 Frog  1370 DRKRLEDDEDRLLATLLHNMIAYMIMMKVNKTDLRRKVRRILGKCHIGLVHSQQINELLDRVTDM 1434

  Fly  1585 NGNDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTY 1649
            ||.|:.::|.|||.:.:|:|.||.|||.:|.|.||||.||.:||||..|.:.||||||:|:||||
 Frog  1435 NGRDLSIRPSGSRHIKKQTFVVHAGTDTSGDLFFMEVCDDCIVLRSNIGAVYERWWYEKLINMTY 1499

  Fly  1650 SPKTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGTPTNV----PELGGEFPVQDMNT 1710
            .||||:||||||||.:|||:|::|:||:|||.|:||:|||.......    ||||||||||||.|
 Frog  1500 CPKTKVLCLWRRNGQETQLNKFFTKKCRELYYCVKESMERAAARQQSIKPGPELGGEFPVQDMKT 1564

  Fly  1711 GEGGLLQVCLEGVGLLFSNS---KFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMS 1772
            ||||||||.|||:.|.|.:|   |.|::|:||:||.|.: |:|||||:.|:|:.::..||::.|:
 Frog  1565 GEGGLLQVTLEGINLKFMHSQERKVFIQLNHIKKCNTVR-GVFVLEEFVPETKEVVSHKYKTPMA 1628

  Fly  1773 DQICYSVLCVFSYVAA 1788
            .:|||||||:||||||
 Frog  1629 HEICYSVLCLFSYVAA 1644

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rab3-GEF	NP_001259560.1	uDENN	10..111	CDD:214824	56/100 (56%)
		DENN	260..486	CDD:214823	151/227 (67%)
		dDENN	613..681	CDD:129037	44/67 (66%)
madd	XP_012816822.1	uDENN	8..98	CDD:214824	56/100 (56%)
		DENN	162..391	CDD:214823	152/229 (66%)