Protein Alignment Rab3-GEF and madd

DIOPT Version :9

• Sequence 1: NP_001259560.1
• Gene: Rab3-GEF / 32442
• FlyBaseID: FBgn0030613
• Length: 2154
• Species: Drosophila melanogaster
• Sequence 2: XP_021327625.1
• Gene: madd / 100150917
• ZFINID: ZDB-GENE-030131-8076
• Length: 1717
• Species: Danio rerio
• Alignment Length: 1957
• Identity: 761/1957 - (38%)
• Similarity: 1028/1957 - (52%)
• Gaps: 404/1957 - (20%)

  Fly     5 QKASLCPRLVDYMAIVGAHTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDMVYFCQP 69
            :|..:||||:||:.:|||.         |.......|.|.||||||..||.|||||.|:|:||||
Zfish     2 EKKKMCPRLLDYLVVVGAR---------QPSNDSVAQTPQLLRRYPLEDHNDFPLPPDVVFFCQP 57

  Fly    70 EGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIERPSSAAGSAGAGNDRPGNG 134
            |||.|:..||.  ::||.|||||.|||||||.||||||:||||..::          .:.||...
Zfish    58 EGCLSIRQRRV--SLRDDTSFVFTLTDKDSGITRYGICLNFYRSFQK----------AHHRPRAE 110

  Fly   135 GPGGHGGGAGGGAGGGGRGGRRSSAFRRESWRKSMERSSDSAFSSDYRSNVAPSDSDRELTSRRD 199
            |.|                                             ...|.:|:..|.|.:.|
Zfish   111 GKG---------------------------------------------EKPAHTDTAVEATEKSD 130

  Fly   200 SDQQRLHSHHSHHQPHHPSASPAVPKLGLMAPSADSESGGSHSP-SPRASRKRTKLRNQSLTSLC 263
            .....|...|        ||.||..  |.:.|.....||  .|| |.|:.|...:.||..|||||
Zfish   131 PSTLTLSGEH--------SAPPAGD--GTLLPGEPGSSG--KSPRSKRSGRIAPQNRNSMLTSLC 183

  Fly   264 IISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVS---DATPSIVL 325
            |:||:|||:||||||:|||:::|.|::..: :|.||.:...||.:|.|.||.:|   ....|.||
Zfish   184 ILSHYPFFSTFRECLYILKRMVDCCSQRLN-QRPGAPKSTQRDTMWRVFTGALSVEEKEKGSQVL 247

  Fly   326 HDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELLGV 390
            .|:||||:|:.|||.:||||.|..||:||||...:...|.|||||.:|||:||||||||||||||
Zfish   248 QDLREIESWVYRLLRSPVPVAGQRRVDVEVLPHELQPALTFALPDPSRFSIVDFPLHLPLELLGV 312

  Fly   391 ETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLAPT 455
            :.||:|...|:.|:||:.||||||||||||||||:|:||||||||||||||||::.|||||||||
Zfish   313 DACLQVLACILLEHKVVLQSRDYNALSMSVMAFVSMIYPLEYMFPVIPLLPTCMASAEQLLLAPT 377

  Fly   456 PFVIGVPASFLVYKKNFRLPDDIWVVDLDSTKLTPPTGGYEEIPPLPEPEGTILKNHLKQAMLLM 520
            |:||||||||.:||.:|::|||:|:||||..|:..|:.. |.:|||||||.:.||.||||.:
Zfish   378 PYVIGVPASFFLYKCDFKMPDDVWLVDLDCNKVIVPSNA-ELLPPLPEPEASELKKHLKQLL--- 438

  Fly   521 DEAGLGALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSAQG 585
              ..|..||.:|......|..:.|.|:  ||...|:|.:.:.           |..|..|:...|
Zfish   439 --ECLVRLTLITQKQIFASDSKALASM--SLNTQPILNLEKF-----------QDGQELSLLPPG 488

  Fly   586 TISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVVAF 650
                |..:..:|...||.:||.||||||||||||||||||:.|.|..|..|||||||:|||||||
Zfish   489 ----RDKASPSSTEFNPLIYGNDVDSVDVATRVAMVRFFNSPNVLQGFQMHTRTLRLFPRPVVAF 549

  Fly   651 QINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHALT 715
            |..|||.||||.:.|..:.:.|||||:..||:|.|||:||.|:...|.||..:||||||:||.|.
Zfish   550 QSTSFLASRPRRNGFTEKLSHTQAVEYYGEWALNPTNLAFQRIHNNVYDPSLIGDKPKWYAHQLQ 614

  Fly   716 PIRFSVWDDGSSLNGALRSLKQLE---CQPTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSP 777
            |:.:.|:|..|.|..||....|.|   ..|||:||||||..|.||||||||.|||:||...|:..
Zfish   615 PVFYRVYDGNSHLAEALSGPLQDETNDSDPTDDSGSDSEAYDDSSSSYSSLGDFVNEMIKGDIQG 679

  Fly   778 SLHDVFGSYNRPHVVPQTLSS--NLDPALVYHPPSKLQYPEGIADAVASKEEEDEERADS-PVSS 839
                     :.|:|.|.|.::  :.|...::      .:.|...|:...:.|...|.||| |:.|
Zfish   680 ---------DTPNVDPLTHAALGDADEVEIH------DFHEYKGDSGEPEPEGPTEAADSQPLRS 729

  Fly   840 SSSRSDLSSPS-----FNRDSEFDFQPKGGQTLGSTTVGSGA---AAPSFELATPLAMRLEATIK 896
            |||.:..||||     .|.:.:   :|...:.:.:.|..|.|   ..|.|...||....::...|
Zfish   730 SSSTTASSSPSTVIQGVNHEQK---EPVEVEPIANVTFPSSAPVLGPPPFTRPTPDPTLVDPANK 791

  Fly   897 MAS-------------IEQESDTVSTATGKTIAAGSKLQRHPSDSESRPEKKIPPPLTP-PVKQP 947
            ...             .|::::|.......|          |..:::....|...||.| .:|.|
Zfish   792 KREYDNPYFEPQYGFPTEEDAETDEQEESYT----------PRFNQNLNGNKPSRPLRPSSLKLP 846

  Fly   948 GVSNILARTGSSGSSSSSPGRQSSQSSL--FENFASHAKELVRETTRQSSQEGLLAHVDKFTLHA 1010
            |.|:     |...|.:|||....|.:|.  |....|.|..|.:.            |...|:|
Zfish   847 GESD-----GEGDSRNSSPNSTISNNSSDGFGGLMSFASNLYKN------------HGTSFSL-- 892

  Fly  1011 KKAAGEASKQALEVSKQAAGVSKNTLEDLTYVGKSTLGDLTKTAKEAATKKGIIK-------IEE 1068
                                              |:|. |...|:|..|....:|       .|:
Zfish   893 ----------------------------------SSLA-LPNKAREKNTPFPSLKEYFNFDLAED 922

  Fly  1069 HSAGGAGPPPKSPGSQLATHKQVQQSGGQGGGNNFFSAIGTDFNGLASSTSTMFSGMFGKKSQQK 1133
            .....|...|:||...|.:..::....|.|.|..                           ::..
Zfish   923 TDQDDADDSPESPVFGLNSLMEIVTEIGPGSGEG---------------------------ARAP 960

  Fly  1134 QVPVQQKQPNVSAGKAKSGINFDPFPGRKGLVERTPLIKHSGPRQTQEELTRQQNQERSHSNAEN 1198
            :..|.||                     ..:::.:|.:|...|          ..|.|:::.:||
Zfish   961 RALVDQK---------------------SSVIKHSPTVKRESP----------SPQGRANNTSEN 994

  Fly  1199 QTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYRTLVLSKLNKTL-DKKIAPDDHIDDVCVTKP 1262
            |.|||:|...||.|:|||||.:.:.::|:|:|..|..||||||:.: .::.|..:.|.||.:.:.
Zfish   995 QQFLKEVVQSVLEGQGVGWLNMKKVRRLLENEQLRVFVLSKLNRAVQSEEDAQQEVIRDVEINRK 1059

  Fly  1263 VWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLMEVAHTHYWSKEINEGSDMSSSLLSSHAA-- 1325
            |:||||..::.....|:.::.|.|||||||||.|:|:|.|||.:|:..:.....:..|||..:
Zfish  1060 VYKGMLDILKCTVSSLEHSYTNAGLGGMASVFSLLEIARTHYQTKDPEKRKRSPTEGLSSPGSKE 1124

  Fly  1326 SPMGSRENLRS-------------PSSPNGSHS----ALGSE--------WASPQESRK-SSTQL 1364
            ||.|..|:.|:             |||...|..    :|..|        |:..|::|| .:.:.
Zfish  1125 SPSGRMESARAAGVLLVPRIQLPPPSSGKSSRQFDTRSLNEENFIASIELWSKHQDNRKQKALEK 1189

  Fly  1365 AHGGPGGSHSGAP-INRRLSSADSQD---------GQSTT----EMFKDMLSQKRSALKNMLTSF 1415
            ...|.....|||. :.:|:...|:::         |.|.|    :...|.|:..........||.
Zfish  1190 EQSGLNKRKSGADGVKQRVEGGDTEEKKSQISADSGLSVTSGSQKSDTDSLASSEPPALTRSTSQ 1254

  Fly  1416 DSDTTTSKDSKKSSGNLWSGKSTLS--AGFRYTG--GHLINTS---------------------- 1454
            ||:.:|...:  |||......|.||  ||...||  |..:|.|
Zfish  1255 DSEASTVVSN--SSGETLGADSDLSSTAGDGLTGRHGQHLNLSRGTLSDSEIETNPATSSVFGKT 1317

  Fly  1455 ---------------SSPSPD----SPRVYLFEGLLGKD---------------------RLNLW 1479
                           ::|:|.    |.|:||.|||||:|                     |..||
Zfish  1318 HKLKPGVKEPVGVNKAAPAPPVEDVSMRIYLCEGLLGRDKSSVWDQLEDAAMETFSLSKERSTLW 1382

  Fly  1480 NQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESERKRLEHDEDRLLSTMLYNLTAILV 1544
            :|||||||||||||..||:.:||||||.||::||.||.:.:|||||.||||||:|:|:|:.|.::
Zfish  1383 DQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRYLSLGDHDRKRLEDDEDRLLATLLHNMIAYML 1447

  Fly  1545 MLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNGNDIDLKPLGSRLLHRQSFTVHQG 1609
            |:.|.|::||:|:|||:|||||||.:|||::.|:|::.:|:|.::.::|.|||.:.:|:|.||.|
Zfish  1448 MMKVGKNDIRKKVRRLMGKSHIGLTHSQEINEVLDRLAHLSGRELSIRPSGSRHIKKQTFVVHAG 1512

  Fly  1610 TDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSPKTKLLCLWRRNGGQTQLHKYYTR 1674
            ||..|.:.||||.||.:||||..||:.||||||:|:||||.||||:||||||||.:|||:|:||:
Zfish  1513 TDTTGDIFFMEVCDDCIVLRSNIGTVYERWWYEKLINMTYCPKTKVLCLWRRNGQETQLNKFYTK 1577

  Fly  1675 KCKELYNCIKEAMERGGTPTNV----PELGGEFPVQDMNTGEGGLLQVCLEGVGLLFSNS---KF 1732
            ||:|||.|:|::|||.......    ||||||||||||.|||||||||.|||:.|.|.:|   |.
Zfish  1578 KCRELYYCVKDSMERAAARQQSIKPGPELGGEFPVQDMKTGEGGLLQVTLEGINLKFMHSQERKV 1642

  Fly  1733 FVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMSDQICYSVLCVFSYVAA--GQDQKKN 1795
            |:.|:||:||.|.| |:|||||:.|:|:.::..||::.|:.||||||||:|||:||  |::.:..
Zfish  1643 FIELNHIKKCNTVK-GVFVLEEFVPETKEVVIHKYKTPMAHQICYSVLCLFSYMAAVKGKESEGK 1706

  Fly  1796 PVVITPQ 1802
            |.:::|:
Zfish  1707 PKMLSPR 1713

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rab3-GEF	NP_001259560.1	uDENN	10..111	CDD:214824	58/100 (58%)
		DENN	260..486	CDD:214823	146/228 (64%)
		dDENN	613..681	CDD:129037	44/67 (66%)
madd	XP_021327625.1	uDENN	7..97	CDD:214824	58/100 (58%)
		DENN	180..406	CDD:214823	144/226 (64%)
		dDENN	512..580	CDD:129037	44/67 (66%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170596025
Domainoid	1	1.000	301	1.000	Domainoid score	I1381
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H14249
Inparanoid	1	1.050	653	1.000	Inparanoid score	I766
OMA	1	1.010	-	-		QHG48534
OrthoDB	1	1.010	-	-		D162625at2759
OrthoFinder	1	1.000	-	-		FOG0006143
OrthoInspector	1	1.000	-	-		otm26175
orthoMCL	1	0.900	-	-		OOG6_106194
Panther	1	1.100	-	-	LDO	PTHR13008
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4435
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	13.910