DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eag and Kcnh1

DIOPT Version :9

Sequence 1:	NP_001036275.1	Gene:	eag / 32428	FlyBaseID:	FBgn0000535	Length:	1270	Species:	Drosophila melanogaster
Sequence 2:	NP_034730.1	Gene:	Kcnh1 / 16510	MGIID:	1341721	Length:	989	Species:	Mus musculus

Alignment Length:	1111	Identity:	534/1111 - (48%)
Similarity:	691/1111 - (62%)	Gaps:	203/1111 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MPGGRRGLVAPQNTFLENIIRRSNSQPDSSFLLANAQIVDFPIVYCNESFCKISGYNRAEVMQKS 65
            |.|||||||||||||||||:||||   |::|:|.||||||:||||.|:.|||:|||:||||||||
Mouse     3 MAGGRRGLVAPQNTFLENIVRRSN---DTNFVLGNAQIVDWPIVYSNDGFCKLSGYHRAEVMQKS 64

  Fly    66 CRYVCGFMYGELTDKETVGRLEYTLENQQQDQFEILLYKKNNLQCGCALSQFGKAQTQETPLWLL 130
            .  .|.|||||||||:||.::..|.||.:.:.||||:||||                 .||:|..
Mouse    65 S--ACSFMYGELTDKDTVEKVRQTFENYEMNSFEILMYKKN-----------------RTPVWFF 110

  Fly   131 LQVAPIRNERDLVVLFLLTFRDITALKQPIDSEDTKGVLGLSKFAKLARSVTRSR----QFSAHL 191
            :::||||||:|.|||||.||.||||.||||:.:..|   |..|||:|.|::|.||    |.:..:
Mouse   111 VKIAPIRNEQDKVVLFLCTFSDITAFKQPIEDDSCK---GWGKFARLTRALTSSRGVLQQLAPSV 172

  Fly   192 PTLKDPTKQSNLAHMMSLSADIMPQYRQEAPKTPPHILLHYCAFKAIWDWVILCLTFYTAIMVPY 256
            ...::..|.|.||.::.|.:||:|||:||||||||||:||||.||..|||:||.|||||||:|||
Mouse   173 QKGENVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHYCVFKTTWDWIILILTFYTAILVPY 237

  Fly   257 NVAFKNKTSEDVSLLVVDSIVDVIFFIDIVLNFHTTFVGPGGEVVSDPKVIRMNYLKSWFIIDLL 321
            ||:||.: ..:|:.|||||||||||.:|||||||||||||.|||:||||:|||||||:||:||||
Mouse   238 NVSFKTR-QNNVAWLVVDSIVDVIFLVDIVLNFHTTFVGPAGEVISDPKLIRMNYLKTWFVIDLL 301

  Fly   322 SCLPYDVFNAFD---------------------------RDEDGIGSLFSALKVVRLLRLGRVVR 359
            |||||||.|||:                           |:..||.||||:||||||||||||.|
Mouse   302 SCLPYDVINAFENVDEVSAFMGDPGKIGFADQIPPPLEGRESQGISSLFSSLKVVRLLRLGRVAR 366

  Fly   360 KLDRYLEYGAAMLILLLCFYMLVAHWLACIWYSIG-----RSDADNGIQYSWLWKLANVTQSPYS 419
            |||.|:|||||:|:||:|.:.|.|||:||||||||     ..|.......|||::||....:||.
Mouse   367 KLDHYIEYGAAVLVLLVCVFGLAAHWMACIWYSIGDYEIFDEDTKTIRNNSWLYQLALDIGTPYQ 431

  Fly   420 YIWSNDTGP-ELVNGPSRKSMYVTALYFTMTCMTSVGFGNVAAETDNEKVFTICMMIIAALLYAT 483
            :   |.:|. :...|||:.|:|:::||||||.:|||||||:|..||.||:|.:.:|:|.:|||||
Mouse   432 F---NGSGSGKWEGGPSKNSVYISSLYFTMTSLTSVGFGNIAPSTDIEKIFAVAIMMIGSLLYAT 493

  Fly   484 IFGHVTTIIQQMTSATAKYHDMLNNVREFMKLHEVPKALSERVMDYVVSTWAMTKGLDTEKVLNY 548
            |||:||||.|||.:.|.:||:|||:||:|:||::|||.||||||||:||||:|::|:||||||..
Mouse   494 IFGNVTTIFQQMYANTNRYHEMLNSVRDFLKLYQVPKGLSERVMDYIVSTWSMSRGIDTEKVLQI 558

  Fly   549 CPKDMKADICVHLNRKVFNEHPAFRLASDGCLRALAMHFMMSHSAPGDLLYHTGESIDSLCFIVT 613
            |||||:||||||||||||.||||||||||||||||||.|...|.|||||:||.|||:|||||:|:
Mouse   559 CPKDMRADICVHLNRKVFKEHPAFRLASDGCLRALAMEFQTVHCAPGDLIYHAGESVDSLCFVVS 623

  Fly   614 GSLEVIQDDEVVAILGKGDVFGDQFWKDSAVGQSAANVRALTYCDLHAIKRDKLLEVLDFYSAFA 678
            |||||||||||||||||||||||.|||::.:.||.||||||||||||.||||.|.:||:||:||:
Mouse   624 GSLEVIQDDEVVAILGKGDVFGDVFWKEATLAQSCANVRALTYCDLHVIKRDALQKVLEFYTAFS 688

  Fly   679 NSFARNLVLTYNLRHRLIFRKVADVKREKELAERRKNEPQLPQNQDHLVRKIFSKFRRTPQVQAG 743
            :||:|||:||||||.|::|||::|||||:|...:||||..|....||.||::|.:||:..:.:..
Mouse   689 HSFSRNLILTYNLRKRIVFRKISDVKREEEERMKRKNEAPLILPPDHPVRRLFQRFRQQKEARLA 753

  Fly   744 SKELVGGS--GQSDVEKGDGEVERTKLPAKLTLTEDARILSTAAAPSPSPSPSPSS--------- 797
            ::.  ||.  ...|||||:...:.|.....|.......:..:.|.|....:.:.|:
Mouse   754 AER--GGRDLDDLDVEKGNALTDHTSANHSLVKASVVTVRESPATPVSFQAATTSTMSDHAKLHA 816

  Fly   798 ------GPPSARSTRASK--WGRLLGSSSVDSASDTSAKVAVSRSLSARESLRESTAQARQSSTS 854
                  ||.:.....|.:  |.|.  ..:.....|.: ||:.:.|:   |:|.|.|
Mouse   817 PGSECLGPKAVSCDPAKRKGWARF--KDACGKGEDWN-KVSKAESM---ETLPERT--------- 866

  Fly   855 SSNGGQGNKVFPKAPKLQASQATLARQDTIDEGGEVDSSPPSRDSRVVIEGAAVSSATVGPSPPV 919
                        |||    .:|||.:.|:.|.|
Mouse   867 ------------KAP----GEATLKKTDSCDSG-------------------------------- 883

  Fly   920 ATTSSAAAGAGVSGGPGSGGTVVAIVTKADRNLALERERQIEMASSRATTSDTYDTGLRETPPTL 984
                                     :||:|  |.|:...:......|:              |.|
Mouse   884 -------------------------ITKSD--LRLDNVGETRSPQDRS--------------PIL 907

  Fly   985 A----------QRDLIATVLDMKVDVRLELQRMQQRIGRIEDLLGELVKRLAPGAGSGGNAPDNS 1039
            |          ::.|.||||::|.:::.:::.:..::..||..|.|:: |:....|| ..:|..:
Mouse   908 AEVKHSFYPIPEQTLQATVLEVKYELKEDIKALNAKMTSIEKQLSEIL-RILMSRGS-AQSPQET 970

  Fly  1040 SGQTTP 1045
            ...:.|
Mouse   971 GEISRP 976

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eag	NP_001036275.1	PAS_9	28..155	CDD:290162	70/126 (56%)
		PAS	40..153	CDD:238075	60/112 (54%)
		Ion_trans	254..499	CDD:278921	158/277 (57%)
		Ion_trans_2	<440..494	CDD:285168	34/53 (64%)
		Crp	565..>702	CDD:223736	104/136 (76%)
		CAP_ED	571..682	CDD:237999	85/110 (77%)
Kcnh1	NP_034730.1	PAS_9	39..135	CDD:290162	62/114 (54%)
		PAS	39..133	CDD:238075	60/112 (54%)
		Required for phosphatidylinositol bisphosphate binding. /evidence=ECO:0000250\|UniProtKB:O95259	151..162		5/10 (50%)
		Ion_trans	235..510	CDD:278921	158/278 (57%)
		Ion_trans_2	<450..504	CDD:285168	34/53 (64%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:Q63472	463..468		4/4 (100%)
		Crp	575..>720	CDD:223736	110/144 (76%)
		CAP_ED	581..691	CDD:237999	84/109 (77%)
		Calmodulin-binding. /evidence=ECO:0000269\|PubMed:22732247, ECO:0000269\|PubMed:27618660	673..770		49/98 (50%)
		Interaction with cyclic nucleotide-binding pocket. /evidence=ECO:0000269\|PubMed:22732247	699..701		1/1 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	857..905		20/148 (14%)
		CAD (involved in subunit assembly). /evidence=ECO:0000250\|UniProtKB:Q63472	924..964		12/41 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	961..989		4/17 (24%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850332
Domainoid	1	1.000	352	1.000	Domainoid score	I1005
eggNOG	1	0.900	-	-		E1_COG2202
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	973	1.000	Inparanoid score	I317
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48089
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000148
OrthoInspector	1	1.000	-	-		otm43035
orthoMCL	1	0.900	-	-		OOG6_104814
Panther	1	1.100	-	-	LDO	PTHR10217
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3522
SonicParanoid	1	1.000	-	-		X71
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.790