DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment rut and Adcy8

DIOPT Version :9

Sequence 1:	NP_511156.2	Gene:	rut / 32406	FlyBaseID:	FBgn0003301	Length:	2248	Species:	Drosophila melanogaster
Sequence 2:	NP_058838.1	Gene:	Adcy8 / 29241	RGDID:	2036	Length:	1248	Species:	Rattus norvegicus

Alignment Length:	1278	Identity:	474/1278 - (37%)
Similarity:	678/1278 - (53%)	Gaps:	241/1278 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 RGRPLNTLR--FENDELECLYQRYTLKLQRFS--VLGVVALVFVLCGVMAALSLTFNNAATFHNI 69
            ||....|||  |::.:||.|||||.|..:|.|  |:.|:.::..|..::..|||    |:...:.
  Rat   147 RGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSL----ASAPMDP 207

  Fly    70 FNAIVCGLFAVVLVLLQCSVI---KDHHLPTLCYGILLFTASICVVSMPT------LGSVFPVDT 125
            ...|:.|.|..:.|:: |:::   ||    |..:..|.::..:..|:|.|      ||
  Rat   208 LKGILLGFFTGIEVVI-CALVVVRKD----TTSHTYLQYSGVVTWVAMTTQILAAGLG------- 260

  Fly   126 KEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANI 190
            ..::.:|:..::|.:|..|:|:||.:..|:..|:....:.::|   ::....|.....||::|.:
  Rat   261 YGLLGDGIGYVLFTLFATYSMLPLPLTWAILAGLGTSLLQVTL---QVLIPRLAVFSINQVLAQV 322

  Fly   191 VIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDI 255
            |:|:.:|.||:.::.:.:||||:.||:||.|:.:||.::.||::.|||:|||||:.|.::|.||:
  Rat   323 VLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDM 387

  Fly   256 LS----PVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHC 316
            .:    .:..|||||||.::|||||||||:.|||.||:..|||||||:|||||.|||:|||::||
  Rat   388 TNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHC 452

  Fly   317 LRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVL 381
            ||||||||||||||||||||:|||.|.|||||.||..|..|...|...::||:|||:|.||||||
  Rat   453 LRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVL 517

  Fly   382 GLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTF 446
            |||||||||||.||.:||.:||||.|||:|:::||||.|||:|.||.|||.||:.:||.|.::|:
  Rat   518 GLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKERNEFLRKHNIETY 582

  Fly   447 FIVPPPHRRKPLMLNTLGVRSAIGSRRK--LSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPS 509
            .|..|......|..:.:....:...||.  .:|...|           :|..:||.||
  Rat   583 LIKQPEESLLSLPEDIVKESVSCSDRRNSGATFTEGS-----------WSPELPFDNI------- 629

  Fly   510 AASALGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKVIRLQKILHATPPPHGMGYGS 574
                                 |.:.:|..|.:.|
  Rat   630 ---------------------VGKQNTLAALTRN------------------------------- 642

  Fly   575 VVGSGGGVDSGISGGGGCVSGGIAGGGGVQVTVGTNPNSTASTISRIHRHNHKNNKSQSKVADKF 639
                                           ::...||..|.
  Rat   643 -------------------------------SINLLPNHLAQ----------------------- 653

  Fly   640 KRPFRKRHSVAAHHQP-TNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLG 703
                      |.|.|. ...:|:.:...|:.||.|..:.||:...:|.|:.|.:|.:|.:..|..
  Rat   654 ----------ALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEV 708

  Fly   704 FTTAMGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWV--SAILMLLLA--------VRLKWI 758
            |.:.:.|:.::|:...|:| :.||.:.::.:...|:.:.:  ||::::..|        :..|..
  Rat   709 FKSNLVCAFIVLLFITAIQ-SLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRKTC 772

  Fly   759 IWDISESFSLRMAITIFTVILIYSVGQV-NVFTCVSDH--PCSG---NGTTSFQNDSHRKCSLPQ 817
            .| |:|::..|..| ||..|||..:|.| |:..|..|.  |...   |.:..|.:    .||.|:
  Rat   773 CW-INETYLARNVI-IFASILINFLGAVINILWCDFDKSIPLKNLTFNSSAVFTD----ICSYPE 831

  Fly   818 YVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNAS----IPLH 878
            |...:...|.::.:|||||..:.|..::|.|..||.|..|..:..:|..||| :|.|    :...
  Rat   832 YFVFTGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDN-LNHSGEDFLGTK 895

  Fly   879 LISLARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHF 943
            ..||..:|:|::|:..||:.:|.||||||||::||.:|..||..|:|.|:.:|.|:||.|||.||
  Rat   896 EASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKDLREHNENMLRNILPGHVARHF 960

  Fly   944 LDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRF 1008
            |:.. |:|.|||.|||..|||:|||:|.|.:||::.:.::||:|||||||||||||||||.||||
  Rat   961 LEKD-RDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRF 1024

  Fly  1009 RGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLR 1073
            :.|:||||:|||||||.||.|| |.|..|...   |:.||.::..|:..|:||||.||:|||.||
  Rat  1025 QDIEKIKTIGSTYMAVSGLSPE-KQQCEDKWG---HLCALADFSLALTESIQEINKHSFNNFELR 1085

  Fly  1074 VGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTI 1138
            :||:.|.||||||||:||||||||.|||:|||||||||.|..||.:|....|....|.|..||.|
  Rat  1086 IGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEI 1150

  Fly  1139 KVKG----KGDMVTYFLCDSGNKSLNGEVR-NAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKK 1198
            .|||    :|.:.||||.        |.|: |...||.. ..|..|              :.:..
  Rat  1151 YVKGISEQEGKIKTYFLL--------GRVQPNPFILPPR-RLPGQY--------------SLAAV 1192

  Fly  1199 ENGHLYAGNGVEEQQLLLQH-------QHKQHDPLPLPAPPPP 1234
            ..|.:.:.|...::|||.::       .|.....|..|:.|.|
  Rat  1193 VLGLVQSLNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEP 1235

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
rut	NP_511156.2	AC_N	<17..255	CDD:292831	76/250 (30%)
		CYCc	230..425	CDD:214485	131/198 (66%)
		Guanylate_cyc	266..438	CDD:278633	121/171 (71%)
		DUF1053	624..696	CDD:283888	14/72 (19%)
		SLC5-6-like_sbd	<742..>893	CDD:294310	50/170 (29%)
		CYCc	924..1134	CDD:214485	126/209 (60%)
		Guanylate_cyc	954..1151	CDD:278633	120/200 (60%)
Adcy8	NP_058838.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000269\|PubMed:16258073, ECO:0000269\|PubMed:22494970, ECO:0000269\|PubMed:22976297	1..179		15/31 (48%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000269\|PubMed:22976297	1..106
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..117
		AC_N	<158..397	CDD:292831	76/257 (30%)
		CYCc	363..561	CDD:214485	131/197 (66%)
		Guanylate_cyc	402..586	CDD:278633	126/183 (69%)
		DUF1053	614..708	CDD:283888	30/227 (13%)
		CYCc	941..1145	CDD:214485	125/208 (60%)
		Guanylate_cyc	970..1169	CDD:278633	122/210 (58%)
		Involved in CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	1106..1248		51/153 (33%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000269\|PubMed:19305019	1197..1212		4/14 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1220..1248		5/16 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100296
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.710