DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment rut and PHLPP1

DIOPT Version :9

Sequence 1:	NP_511156.2	Gene:	rut / 32406	FlyBaseID:	FBgn0003301	Length:	2248	Species:	Drosophila melanogaster
Sequence 2:	NP_919431.2	Gene:	PHLPP1 / 23239	HGNCID:	20610	Length:	1717	Species:	Homo sapiens

Alignment Length:	1478	Identity:	251/1478 - (16%)
Similarity:	435/1478 - (29%)	Gaps:	592/1478 - (40%)

- Green bases have known domain annotations that are detailed below.

  Fly   494 SEPVPFSNIATGSFP--SAASALGGGVSVGGGGGGGGGGVARGSTCEANS------GNVQVSEKG 550
            ::|:|....|:...|  .|..|:           ...||..|..:||..:      |.:| |..|
Human   401 AQPLPLPQTASSPQPQQKAPRAI-----------DSPGGAVREGSCEEKAAAAVAPGGLQ-STPG 453

  Fly   551 SRKVIRLQKILHATPPP------HG----------------------MGYGSVVG-SGGGVDSGI 586
             |..:..:|.....|||      ||                      :|:|.:.. ...|:||.|
Human   454 -RSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEI 517

  Fly   587 SGGGGCVSGGIAGGGGVQVTVGTNPNSTASTISRIH---RHNHKNNKSQ---------------- 632
                ||:....||          .|:||.|: .||.   .:|.:..|.|
Human   518 ----GCLIRFYAG----------KPHSTGSS-ERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGT 567

  Fly   633 ----SKVAD--------------KFKRPFRKRHSVA-AHHQPTN------------------RVN 660
                |.|.|              |.:...:.:|.:| :...|.:                  :|:
Human   568 CLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVS 632

  Fly   661 RFLSQAINARSVDCDKSEHV--------DRLTLRFRQS--------------------------D 691
            :..||.|::..:.|...||:        |...|..:|:                          :
Human   633 KVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLN 697

  Fly   692 MEREYHKDFDLGFTTA-------MGCSLL---------------LLILGAALQVTALP------R 728
            :...:..||.|...:.       :.|:.|               .|:.|..||  :||      :
Human   698 LSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQ--SLPAELENMK 760

  Fly   729 TLILLLLFLFAFIWVSAILMLLLAVRLKWIIWDISESFSLR------------MAITIFTVILIY 781
            .|..|.|....|..:..:|..|.||....:..:..|:..|:            :.:.:...::..
Human   761 QLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIAD 825

  Fly   782 SVGQVNVFTCVS--DHPCSGNGTTSFQNDSHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLL 844
            .|..:...|.:.  |:.........|.|.....|...|.|:|.....||..        ::.|..
Human   826 EVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKA--------LYASSN 882

  Fly   845 VLGMGTIYGLFIELSHQNIFECYDNRVNASIPLHLISLARIAIFMIAILVHGRLVEGTARL---- 905
            .|....:|.:...||:.::..   ||:. ::|..:....::.:..|.   |.::.|..|||
Human   883 ELVQLDVYPVPNYLSYMDVSR---NRLE-NVPEWVCESRKLEVLDIG---HNQICELPARLFCNS 940

  Fly   906 ---------DFLWQLQASQEKKEMDVLQESNKRILH---NLLPAHVAAHFLDAQ----------- 947
                     :.|.:|....|:..::||...:.::|.   |||....:..||:|.
Human   941 SLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPAT 1005

  Fly   948 -------------FRNN---------------MELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQ 984
                         ..||               :::.|.:|.::....||.....|...|:|.|..
Human  1006 LSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGN 1070

  Fly   985 GLECL-------RLLNEIIAD------FDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEY---KI 1033
            .|:.:       |.::.:||.      |.|:::....:.:|    :....::.|.| ||.   |:
Human  1071 KLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVD----LSCNELSEVTL-PENLPPKL 1130

  Fly  1034 QPND----PNSVRRHMT-ALIEYVKAMR---------HSLQEINSHSY----------------- 1067
            |..|    |..|..|.| .|:..::..:         .....:.||.|
Human  1131 QELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSV 1195

  Fly  1068 NNF---------------------MLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDST-- 1109
            |||                     :|:  ..:..::|..:...|.:.:...||..|..|...|
Human  1196 NNFCDNREALYGVFDGDRNVEVPYLLQ--CTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAG 1258

  Fly  1110 ----GVPGYSQVTQEVVDSLVGSHFEF------RCRGTIKVKGKGDMV--TYFL-CDSGNKSL-- 1159
                |......:..:.||.  |..|..      :|:..:...||...:  :|.: |:...|.:
Human  1259 QKLGGAAVLCHIKHDPVDP--GGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQ 1321

  Fly  1160 -------NGEVRNAMSLPQSLHAPDY-YMKVSQFPENRVNTDTYSKKENGHLYAGNG------VE 1210
                   :|:|.   .:.:|.....| ::..|..|...|.:...:.::...:....|      ||
Human  1322 HKAIITEDGKVN---GVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVE 1383

  Fly  1211 E----------------QQLLLQHQHKQHDPL-------------------------PLPAP--- 1231
            |                :...|...:..||.:                         |.|:|
Human  1384 EAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIF 1448

  Fly  1232 PPPVHHHLHQQQQQRLNSKLQKQPIFMANG-GLPNIRENGNGHNGEHQQQQQQQQQHQQQQQQQQ 1295
            ||.|            |..::.:|   ::| |:|:   :.:|...|...:....:...:.
Human  1449 PPSV------------NMVIKDRP---SDGLGVPS---SSSGMASEISSELSTSEMSSEV----- 1490

  Fly  1296 QHGGFMVATTTPPAAVA--VPLQPQHHQLQFQHPHQHPLPSAVSVPVQHQILLHHQLQLQ----- 1353
               |...:...||.|::  .|..|...:...                 |.|.|.:..|.|
Human  1491 ---GSTASDEPPPGALSENSPAYPSEQRCML-----------------HPICLSNSFQRQLSSAT 1535

  Fly  1354 --------------HQPVPSVMLREFNIIENPTSGGR-----------------HQQMEQLPPHH 1387
                          .:|:..|.          |:|.|                 .|.:.|:|
Human  1536 FSSAFSDNGLDSDDEEPIEGVF----------TNGSRVEVEVDIHCSRAKEKEKQQHLLQVP--- 1587

  Fly  1388 GSLDLSGMGMGVGAGVLGSDCFMMPRRDRERTYVPPLNQHGHHPPHHLHSNLNLNQSQHPPSFTS 1452
              .:.|..|:.:.|   ..|...:||:                                 ..|::
Human  1588 --AEASDEGIVISA---NEDEPGLPRK---------------------------------ADFSA 1614

  Fly  1453 LG-YGQCR---------ESEPLLHASSVAPVAK-----IMPMQHAPKYE---PPRYTSPHTMLSQ 1499
            :| .|:.|         .|..::..::.||:.|     ..|.|..|..:   ||........:.:
Human  1615 VGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMK 1679

  Fly  1500 QHQQQQQQQHQHQQPQSQSAQDQQTHPAQDPHPLQRQYAMYSQQPQLP 1547
            .||:|||||.....||                 ||.|...:.|..|||
Human  1680 HHQEQQQQQQPPPPPQ-----------------LQPQLPRHYQLDQLP 1710

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
rut	NP_511156.2	AC_N	<17..255	CDD:292831
		CYCc	230..425	CDD:214485
		Guanylate_cyc	266..438	CDD:278633
		DUF1053	624..696	CDD:283888	20/158 (13%)
		SLC5-6-like_sbd	<742..>893	CDD:294310	26/164 (16%)
		CYCc	924..1134	CDD:214485	55/331 (17%)
		Guanylate_cyc	954..1151	CDD:278633	50/278 (18%)
PHLPP1	NP_919431.2	leucine-rich repeat	1107..1129	CDD:275380	5/26 (19%)
		PP2Cc	1168..1420	CDD:214625	39/258 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1458..1510		11/65 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1673..1717		16/55 (29%)
		PDZ-binding, required for interaction with SLC9A3R1. /evidence=ECO:0000269\|PubMed:21804599	1715..1717
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..25
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	41..118
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	136..156
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	252..470		19/81 (23%)
		PH_PHLPP-like	535..631	CDD:270131	12/95 (13%)
		PH	555..636	CDD:278594	8/80 (10%)
		LRR_RI	625..929	CDD:238064	52/320 (16%)
		LRR_8	638..703	CDD:290566	7/64 (11%)
		LRR 1	638..659		4/20 (20%)
		leucine-rich repeat	641..661	CDD:275380	3/19 (16%)
		LRR 2	661..682		3/20 (15%)
		leucine-rich repeat	662..692	CDD:275380	2/29 (7%)
		LRR 3	692..712		3/19 (16%)
		leucine-rich repeat	693..738	CDD:275380	5/44 (11%)
		LRR_8	714..770	CDD:290566	11/57 (19%)
		LRR 4	715..736		2/20 (10%)
		LRR 5	738..760		6/23 (26%)
		leucine-rich repeat	739..761	CDD:275380	6/23 (26%)
		LRR 6	761..783		5/21 (24%)
		leucine-rich repeat	762..784	CDD:275380	6/21 (29%)
		LRR 7	784..804		4/19 (21%)
		leucine-rich repeat	785..808	CDD:275380	3/22 (14%)
		LRR 8	808..831		1/22 (5%)
		leucine-rich repeat	810..832	CDD:275380	1/21 (5%)
		LRR_RI	814..1074	CDD:238064	48/274 (18%)
		LRR 9	832..853		3/20 (15%)
		leucine-rich repeat	833..853	CDD:275380	3/19 (16%)
		leucine-rich repeat	854..895	CDD:275380	9/48 (19%)
		LRR 10	873..894		5/28 (18%)
		LRR 11	895..916		5/24 (21%)
		leucine-rich repeat	896..918	CDD:275380	5/25 (20%)
		LRR 12	918..939		6/23 (26%)
		leucine-rich repeat	919..941	CDD:275380	6/24 (25%)
		LRR 13	941..962		3/20 (15%)
		leucine-rich repeat	942..963	CDD:275380	3/20 (15%)
		LRR_8	962..1024	CDD:290566	11/61 (18%)
		LRR 14	963..984		6/20 (30%)
		leucine-rich repeat	964..987	CDD:275380	6/22 (27%)
		LRR 15	987..1008		3/20 (15%)
		leucine-rich repeat	988..1011	CDD:275380	3/22 (14%)
		LRR 16	1013..1033		2/19 (11%)
		LRR_8	1014..1072	CDD:290566	10/57 (18%)
		leucine-rich repeat	1014..1037	CDD:275380	2/22 (9%)
		LRR 17	1037..1058		4/20 (20%)
		leucine-rich repeat	1038..1061	CDD:275380	4/22 (18%)
		LRR 18	1061..1082		4/20 (20%)
		Interaction with SLC9A3R1. /evidence=ECO:0000269\|PubMed:21804599	1076..1205		24/133 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.900