DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment rut and Adcy8

DIOPT Version :9

Sequence 1:	NP_511156.2	Gene:	rut / 32406	FlyBaseID:	FBgn0003301	Length:	2248	Species:	Drosophila melanogaster
Sequence 2:	NP_033753.2	Gene:	Adcy8 / 11514	MGIID:	1341110	Length:	1249	Species:	Mus musculus

Alignment Length:	1274	Identity:	473/1274 - (37%)
Similarity:	679/1274 - (53%)	Gaps:	233/1274 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 RGRPLNTLR--FENDELECLYQRYTLKLQRFS--VLGVVALVFVLCGVMAALSLTFNNAATFHNI 69
            ||....|||  |::.:||.|||||.|..:|.|  |:.|:.::..|..::..|||    |:...:.
Mouse   148 RGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSL----ASAPMDP 208

  Fly    70 FNAIVCGLFAVVLVLLQCSVI---KDHHLPT-LCY-GILLFTASICVVSMPTLGSVFPVDTKEVM 129
            ...|:.|.|..:.|:: |:::   ||:...| |.| |::.:.|....:....||       ..::
Mouse   209 LKGILLGFFTGIEVVI-CALVVVRKDNTSHTYLQYSGVVTWVAMTTQILAAGLG-------YGLL 265

  Fly   130 AEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFI 194
            .:|:..::|.:|..|:|:||.:..|:..|:....:.::|   ::....|.....||::|.:|:|:
Mouse   266 GDGIGYVLFTLFATYSMLPLPLTWAILAGLGTSLLQVTL---QVLIPRLAVFSINQVLAQVVLFM 327

  Fly   195 GVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILS-- 257
            .:|.||:.::.:.:||||:.||:||.|:.:||.::.||::.|||:|||||:.|.::|.||:.:
Mouse   328 CMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVE 392

  Fly   258 --PVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIK 320
              .:..|||||||.::|||||||||:.|||.||:..|||||||:|||||.|||:|||::||||||
Mouse   393 DEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIK 457

  Fly   321 ILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRK 385
            ||||||||||||||||:|||.|.|||||.||..|..|...|...::||:|||:|.||||||||||
Mouse   458 ILGDCYYCVSGLPEPRRDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRK 522

  Fly   386 WQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVP 450
            |||||||.||.:||.:||||.|||:|:::||||.|:|:|.||.|||.||:.:||.|.::|:.|..
Mouse   523 WQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLNGDYNVEEGHGKERNEFLRKHNIETYLIKQ 587

  Fly   451 PPHRRKPLMLNTLGVRSAIGSRRK--LSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAASA 513
            |......|..:.:....:...||.  .:|...|           :|..:||.||
Mouse   588 PEESLLCLPEDIVKESVSCSDRRNSGATFTEGS-----------WSPELPFDNI----------- 630

  Fly   514 LGGGVSVGGGGGGGGGGVARGSTCEANSGNVQVSEKGSRKVIRLQKILHATPPPHGMGYGSVVGS 578
                             |.:.:|..|.:.|
Mouse   631 -----------------VGKQNTLAALTRN----------------------------------- 643

  Fly   579 GGGVDSGISGGGGCVSGGIAGGGGVQVTVGTNPNSTASTISRIHRHNHKNNKSQSKVADKFKRPF 643
                                       ::...||..|.
Mouse   644 ---------------------------SINLLPNHLAQ--------------------------- 654

  Fly   644 RKRHSVAAHHQP-TNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTA 707
                  |.|.|. ...:|:.:...|:.||.|..:.||:...:|.|:.|.:|.:|.:..|..|.:.
Mouse   655 ------ALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSN 713

  Fly   708 MGCSLLLLILGAALQVTALPRTLILLLLFLFAFIWV--SAILMLLLA--------VRLKWIIWDI 762
            :.|:.::|:...|:| :.||.:.::.:...|:.:.:  ||::::..|        :..|...| |
Mouse   714 LVCAFIVLLFITAIQ-SLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRKTCCW-I 776

  Fly   763 SESFSLRMAITIFTVILIYSVGQV-NVFTCVSDH--PCSG---NGTTSFQNDSHRKCSLPQYVSL 821
            :|::..|..| ||..|||..:|.| |:..|..|.  |...   |.:..|.:    .||.|:|...
Mouse   777 NETYLARNVI-IFASILINFLGAVLNILWCDFDKSIPLKNLTFNSSAVFTD----ICSYPEYFVF 836

  Fly   822 SAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDNRVNAS----IPLHLISL 882
            :...|.::.:|||||..:.|..::|.|..||.|..|..:..:|..||| :|.|    :.....||
Mouse   837 TGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDN-LNHSGEDFLGTKEASL 900

  Fly   883 ARIAIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQ 947
            ..:|:|::|:..||:.:|.||||||||::||.:|..||..|:|.|:.:|.|:||:|||.|||:..
Mouse   901 LLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARHFLEKD 965

  Fly   948 FRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGID 1012
             |:|.|||.|||..|||:|||:|.|.:||::.:.::||:|||||||||||||||||.||||:.|:
Mouse   966 -RDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIE 1029

  Fly  1013 KIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALIEYVKAMRHSLQEINSHSYNNFMLRVGIN 1077
            ||||:|||||||.||.|| |.|..|...   |:.||.::..|:..|:||||.||:|||.||:||:
Mouse  1030 KIKTIGSTYMAVSGLSPE-KQQCEDKWG---HLCALADFSLALTESIQEINKHSFNNFELRIGIS 1090

  Fly  1078 IGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKG 1142
            .|.||||||||:||||||||.|||:|||||||||.|..||.:|....|....|.|..||.|.|||
Mouse  1091 HGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKG 1155

  Fly  1143 ----KGDMVTYFLCDSGNKSLNGEVR-NAMSLPQSLHAPDYYMKVSQFPENRVNTDTYSKKENGH 1202
                :|.:.||||.        |.|: |...||.. ..|..|              :.:....|.
Mouse  1156 ISEQEGKIKTYFLL--------GRVQPNPFILPPR-RLPGQY--------------SLAAVVLGL 1197

  Fly  1203 LYAGNGVEEQQLLLQH-------QHKQHDPLPLPAPPPP 1234
            :.:.|...::|||.::       .|.....|..|:.|.|
Mouse  1198 VQSLNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEP 1236

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
rut	NP_511156.2	AC_N	<17..255	CDD:292831	76/246 (31%)
		CYCc	230..425	CDD:214485	130/198 (66%)
		Guanylate_cyc	266..438	CDD:278633	120/171 (70%)
		DUF1053	624..696	CDD:283888	14/72 (19%)
		SLC5-6-like_sbd	<742..>893	CDD:294310	50/170 (29%)
		CYCc	924..1134	CDD:214485	126/209 (60%)
		Guanylate_cyc	954..1151	CDD:278633	120/200 (60%)
Adcy8	NP_033753.2	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..180		15/31 (48%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1..107
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	14..33
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	50..118
		AC_N	<159..398	CDD:292831	76/253 (30%)
		CYCc	364..562	CDD:214485	130/197 (66%)
		Guanylate_cyc	403..587	CDD:278633	125/183 (68%)
		DUF1053	615..709	CDD:283888	30/227 (13%)
		CYCc	942..1146	CDD:214485	125/208 (60%)
		Guanylate_cyc	971..1170	CDD:278633	122/210 (58%)
		Involved in CALM1 interaction. /evidence=ECO:0000250\|UniProtKB:P40146	1107..1249		51/153 (33%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000250\|UniProtKB:P40146	1198..1213		4/14 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1221..1249		5/16 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100296
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.710