DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NetB and unc-6

DIOPT Version :9

Sequence 1:	NP_001162753.1	Gene:	NetB / 32400	FlyBaseID:	FBgn0015774	Length:	793	Species:	Drosophila melanogaster
Sequence 2:	NP_509165.1	Gene:	unc-6 / 180961	WormBaseID:	WBGene00006746	Length:	612	Species:	Caenorhabditis elegans

Alignment Length:	795	Identity:	300/795 - (37%)
Similarity:	411/795 - (51%)	Gaps:	217/795 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 RMGLLLPIILALAIGSSAAGIS----SNDPCY-FEGKPRKCLPSFVNAAYGNPVQASSVCGAQQP 67
            |..|.|...:.:|.|:..:..|    .:|||: ..|:|.:|:|.|:|||:|.||.||..||..:|
Worm     7 RYVLALYFCMGIAHGAYFSQFSMRAPDHDPCHDHTGRPVRCVPEFINAAFGKPVIASDTCGTNRP 71

  Fly    68 ERYCELLRDGNAG----ECRSCEQQRY---GPAA-LTDLNNPSNVTCWRSGAVNVPHDPDSAPPD 124
            ::|| .:::|..|    :|.:|:.:.:   .||: |||||:..|:|||    |:.|    |..|.
Worm    72 DKYC-TVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGNMTCW----VSTP----SLSPQ 127

  Fly   125 NVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKI 189
            ||:||||||||:||||:|:.||.|  .|||:|::||:|||:||.|||||||:|::.:||.....|
Worm   128 NVSLTLSLGKKFELTYVSMHFCSR--LPDSMALYKSADFGKTWTPFQFYSSECRRIFGRDPDVSI 190

  Fly   190 SKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQ 254
            :|.|||||.| .:.|..|....|.||..||.||||.:.::|.|||||||||||:|||.||
Worm   191 TKSNEQEAVC-TASHIMGPGGNRVAFPFLENRPSAQNFENSPVLQDWVTATDIKVVFSRL----- 249

  Fly   255 LLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDD 319
                                 ..|.|:|          |.|.::.::
Worm   250 ---------------------SPDQAEL----------YGLSNDVNS------------------ 265

  Fly   320 DHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAAS 384
                  |.::.:..||::
Worm   266 ------YGNETDDEVKQR----------------------------------------------- 277

  Fly   385 AVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGR 449
                   ..|::.:.|||||||||||||.|:                            .:..||
Worm   278 -------YFYSMGELAVGGRCKCNGHASRCI----------------------------FDKMGR 307

  Fly   450 STQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELY 514
                     .||.|||||||.|||.||||::||||||||.|.||.|..|.||.||:||||:.||:
Worm   308 ---------YTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELF 363

  Fly   515 KLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQ 579
            :|||..|||||.||:|:|.||.||.|:.|:.||.:.|..|||.||.|.||||||.||:||..|||
Worm   364 RLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKSCNQSSGQ 428

  Fly   580 CPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEE 644
            |.||.||||.||||||:||||:||.|.||||:||.|:.|                    ||...|
Worm   429 CVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKADFI--------------------GSSHSE 473

  Fly   645 MKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIF 709
            .:..|.||:..||:||..|||..|:|:...|:              |.|..:...||:|.::::|
Worm   474 EQDQCSKCRIVPKRLNQKKFCKRDHAVQMVVV--------------SREMVDGWAKYKIVVESVF 524

  Fly   710 KRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGR-DSEAAPGYLAIGPSSVVLE 773
            ||    ||.:  :.||...|.:..:.:.|:|||:::.:.||:||: ||:.....|.:.|.:|::|
Worm   525 KR----GTEN--MQRGETSLWISPQGVICKCPKLRVGRRYLLLGKNDSDHERDGLMVNPQTVLVE 583

  Fly   774 WKDEWSLRMKRFQRR 788
            |:|:...::.||.::
Worm   584 WEDDIMDKVLRFSKK 598

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NetB	NP_001162753.1	LamNT	37..261	CDD:214532	112/231 (48%)
		EGF_Lam	<458..484	CDD:238012	18/25 (72%)
		EGF_Lam	498..555	CDD:238012	32/56 (57%)
		EGF_Lam	560..608	CDD:238012	34/47 (72%)
		NTR_netrin-1_like	661..791	CDD:239634	37/129 (29%)
unc-6	NP_509165.1	LamNT	41..289	CDD:214532	123/373 (33%)
		EGF_Lam	290..333	CDD:238012	30/79 (38%)
		Laminin_EGF	347..400	CDD:278482	31/52 (60%)
		Laminin_EGF	410..453	CDD:278482	32/42 (76%)
		NTR_netrin-1_like	490..604	CDD:239634	37/129 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160159484
Domainoid	1	1.000	212	1.000	Domainoid score	I1617
eggNOG	1	0.900	-	-		E1_KOG3512
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H21008
Inparanoid	1	1.050	559	1.000	Inparanoid score	I595
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48023
OrthoDB	1	1.010	-	-		D368755at33208
OrthoFinder	1	1.000	-	-		FOG0003523
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_104609
Panther	1	1.100	-	-	O	PTHR10574
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2886
SonicParanoid	1	1.000	-	-		X1381
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.840