Protein Alignment NetB and unc-6

DIOPT Version :10

• Sequence 1: NP_511155.1
• Gene: NetB / 32400
• FlyBaseID: FBgn0015774
• Length: 793
• Species: Drosophila melanogaster
• Sequence 2: NP_509165.1
• Gene: unc-6 / 180961
• WormBaseID: WBGene00006746
• Length: 612
• Species: Caenorhabditis elegans
• Alignment Length: 795
• Identity: 300/795 - (37%)
• Similarity: 411/795 - (51%)
• Gaps: 217/795 - (27%)

  Fly     8 RMGLLLPIILALAIGSSAAGIS----SNDPCY-FEGKPRKCLPSFVNAAYGNPVQASSVCGAQQP 67
            |..|.|...:.:|.|:..:..|    .:|||: ..|:|.:|:|.|:|||:|.||.||..||..:|
 Worm     7 RYVLALYFCMGIAHGAYFSQFSMRAPDHDPCHDHTGRPVRCVPEFINAAFGKPVIASDTCGTNRP 71

  Fly    68 ERYCELLRDGNAG----ECRSCEQQRY---GPAA-LTDLNNPSNVTCWRSGAVNVPHDPDSAPPD 124
            ::|| .:::|..|    :|.:|:.:.:   .||: |||||:..|:|||    |:.|    |..|.
 Worm    72 DKYC-TVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGNMTCW----VSTP----SLSPQ 127

  Fly   125 NVTLTLSLGKKYELTYISLSFCPRSPRPDSLAIFKSSDFGQTWQPFQFYSSQCQKFYGRPDRAKI 189
            ||:||||||||:||||:|:.||.|  .|||:|::||:|||:||.|||||||:|::.:||.....|
 Worm   128 NVSLTLSLGKKFELTYVSMHFCSR--LPDSMALYKSADFGKTWTPFQFYSSECRRIFGRDPDVSI 190

  Fly   190 SKFNEQEARCINSQHDTGGAAQRFAFNTLEGRPSANDLDSSLVLQDWVTATDIRVVFHRLELPPQ 254
            :|.|||||.| .:.|..|....|.||..||.||||.:.::|.|||||||||||:|||.||
 Worm   191 TKSNEQEAVC-TASHIMGPGGNRVAFPFLENRPSAQNFENSPVLQDWVTATDIKVVFSRL----- 249

  Fly   255 LLKVKNANAFSDEMGGSREEDEDDDADLELDGEQDEYDYNLQDNDSADAGYDEYEEPKKHLELDD 319
                                 ..|.|:|          |.|.::.::
 Worm   250 ---------------------SPDQAEL----------YGLSNDVNS------------------ 265

  Fly   320 DHLHLDYASDGESVVKRQGKHKGSAYEKHYQSKLAATTPPQQPPKVTPPGKVTPPSTAAPSAAAS 384
                  |.::.:..||::
 Worm   266 ------YGNETDDEVKQR----------------------------------------------- 277

  Fly   385 AVTLPISQHYAVSDFAVGGRCKCNGHASECVATVSSGSGTALSDQDDGQDEDTPSAPSLANHFGR 449
                   ..|::.:.|||||||||||||.|:                            .:..||
 Worm   278 -------YFYSMGELAVGGRCKCNGHASRCI----------------------------FDKMGR 307

  Fly   450 STQMSAKLTMTCACKHNTAGPECERCKPFYFDRPWGRATDNDANECKMCQCNGHARRCRFNLELY 514
                     .||.|||||||.|||.||||::||||||||.|.||.|..|.||.||:||||:.||:
 Worm   308 ---------YTCDCKHNTAGTECEMCKPFHYDRPWGRATANSANSCVACNCNQHAKRCRFDAELF 363

  Fly   515 KLSGRVSGGVCYNCQHDTTGRYCHYCREGYYRDATKPPNHRKVCKRCDCHPVGSTGKTCNHLSGQ 579
            :|||..|||||.||:|:|.||.||.|:.|:.||.:.|..|||.||.|.||||||.||:||..|||
 Worm   364 RLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKSCNQSSGQ 428

  Fly   580 CPCKEGVTGLTCNRCARGYQQTRSHVAPCIKVPTNANMIQAESAGGGGGGGTGDYKDGGGSQVEE 644
            |.||.||||.||||||:||||:||.|.||||:||.|:.|                    ||...|
 Worm   429 CVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKADFI--------------------GSSHSE 473

  Fly   645 MKKYCGKCKASPKKLNLNKFCMEDYAILAKVIGHDRASQDISTEKFSIERQNEIYKYEINIQTIF 709
            .:..|.||:..||:||..|||..|:|:...|:              |.|..:...||:|.::::|
 Worm   474 EQDQCSKCRIVPKRLNQKKFCKRDHAVQMVVV--------------SREMVDGWAKYKIVVESVF 524

  Fly   710 KRNPMSGTTSSLLGRGNMMLLVPRKSIECQCPKIKLNKSYLILGR-DSEAAPGYLAIGPSSVVLE 773
            ||    ||.:  :.||...|.:..:.:.|:|||:::.:.||:||: ||:.....|.:.|.:|::|
 Worm   525 KR----GTEN--MQRGETSLWISPQGVICKCPKLRVGRRYLLLGKNDSDHERDGLMVNPQTVLVE 583

  Fly   774 WKDEWSLRMKRFQRR 788
            |:|:...::.||.::
 Worm   584 WEDDIMDKVLRFSKK 598

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NetB	NP_511155.1	LamNT	37..261	CDD:214532	112/231 (48%)
		EGF_Lam	<458..484	CDD:238012	18/25 (72%)
		EGF_Lam	498..555	CDD:238012	32/56 (57%)
		EGF_Lam	560..608	CDD:238012	34/47 (72%)
		NTR_netrin-1_like	661..791	CDD:239634	37/129 (29%)
unc-6	NP_509165.1	LamNT	41..289	CDD:214532	123/373 (33%)
		EGF_Lam	290..333	CDD:238012	30/79 (38%)
		Laminin_EGF	347..400	CDD:395007	31/52 (60%)
		Laminin_EGF	410..453	CDD:395007	32/42 (76%)
		NTR_netrin-1_like	490..604	CDD:239634	37/129 (29%)