Protein Alignment Prp16 and DHX36

DIOPT Version :10

• Sequence 1: NP_572947.1
• Gene: Prp16 / 32373
• FlyBaseID: FBgn0026713
• Length: 1222
• Species: Drosophila melanogaster
• Sequence 2: NP_065916.2
• Gene: DHX36 / 170506
• HGNCID: 14410
• Length: 1008
• Species: Homo sapiens
• Alignment Length: 1162
• Identity: 299/1162 - (25%)
• Similarity: 496/1162 - (42%)
• Gaps: 295/1162 - (25%)

  Fly   189 DRHRSWDRDRGRDRDRDRSMSERSVHTPREPGTPGGSSGGISNSSWDDEDGEFGQRKSDWDMPTP 253
            |.|::|.||.|                ||..|  ||..||                      |..
Human     4 DYHQNWGRDGG----------------PRSSG--GGYGGG----------------------PAG 28

  Fly   254 RRHGNK-SGDWSVRSGGSRRNHGRQDDTVRPTPAHRYNQWAHGRKRSGATPWGEDPESLDLW--- 314
            ...||: ||......||.|...||.       |.|     ..||:             :.:|
Human    29 GHGGNRGSGGGGGGGGGGRGGRGRH-------PGH-----LKGRE-------------IGMWYAK 68

  Fly   315 -----EEEQRRLDREWYNIDEGYDDENNPFGGPNSEYFRKREEQLEQKRTKRISAQQRQNNRDNE 374
                 .:|..|.:|...::||                  :||||:.|    .:::.|.:|::::|
Human    69 KQGQKNKEAERQERAVVHMDE------------------RREEQIVQ----LLNSVQAKNDKESE 111

  Fly   375 LWERNRMLTSGVVTLISVNDDFDEEALERVHLLVHHIIPPFLDGRIVFTKQPEPVVPVKDPTSDM 439
                      ..::..:..|              |..      |..|.||.    .|..:...|:
Human   112 ----------AQISWFAPED--------------HGY------GTEVSTKN----TPCSENKLDI 142

  Fly   440 ALLARKGSALVRNYREQKERRKAQKKHWELSGTKLGNIMGVQRPQDEDDMRFDKEKDKADYRKDQ 504
                           ::|:....:||.:.:.          .|...:.|..:..::::.|...||
Human   143 ---------------QEKKLINQEKKMFRIR----------NRSYIDRDSEYLLQENEPDGTLDQ 182

  Fly   505 KFADHMRDQDTGGKSDFSRKKTISEQRRFLPVFASRQELLNVIRENSVIIIVGETGSGKTTQLTQ 569
            |..:.::.:    |:|. |...:...|..||.:..::||:|:|..:.|.:|.||||.|||||:||
Human   183 KLLEDLQKK----KNDL-RYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQVTQ 242

  Fly   570 YLHEDGYSKRG-----MIGCTQPRRVAAMSVAKRVSDEMDTQL--GEDVGYAIRFED-CTSERTV 626
            :: .|.|.:||     .|.||||||::|:|||:||:.|.....  |...||.||.:. ...::..
Human   243 FI-LDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQIRLQSRLPRKQGS 306

  Fly   627 IKYMTDGILLRESLRDPELDSYSAIIMDEAHERSLSTDVLFGLLREIVARRHDLKLIVTSATMDS 691
            |.|.|.||:|:....||.|.|.|.|::||.|||:|.:|||..::::::..|.|||:|:.|||:::
Human   307 ILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDLKVILMSATLNA 371

  Fly   692 SKFATFFGNVPTFTIPGRTFPV------DVM---------------FSKNTCEDYV---ESAVKQ 732
            .||:.:|||.|...|||.||||      ||:               |.:...:.:|   |...|:
Human   372 EKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRSQFKRGFMQGHVNRQEKEEKE 436

  Fly   733 AL------------------------------------------QVHLTPNEGDMLIFMPGQEDI 755
            |:                                          .:.|...:|.:|:|:||.::|
Human   437 AIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDGAILVFLPGWDNI 501

  Fly   756 EVTCEVLEERLAEIDNAPALSILPIYSQLPSDLQAKIFQKSSDGLRKCVVATNIAETSLTVDGII 820
            ....::|..::  :..:....|:|::|.:|:..|.::|:::..|:||.|:||||||||:|:|.::
Human   502 STLHDLLMSQV--MFKSDKFLIIPLHSLMPTVNQTQVFKRTPPGVRKIVIATNIAETSITIDDVV 564

  Fly   821 YVIDSGYCKLKVYNPRIGMDALQIYPISQANANQRSGRAGRTGPGQAYRLYT--QRQYKDELLAL 883
            ||||.|..|...::.:..:..:....:|:|||.||.|||||..||..|.||.  :....|:   .
Human   565 YVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDD---Y 626

  Fly   884 TVPEIQRTNLANTVLLLKSLGVVDLLQF--HFMDPPPQDNILNSLYQLWILGALDHTGALTTLGR 946
            .:|||.||.|....|.:|.|.:..:..|  ..||||..:.:|.|:..|..|.|||....||.||.
Human   627 QLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDKQEELTPLGV 691

  Fly   947 QMAEFPLDPPQCQMLIVACRMGCSAEVLIIVSMLSVPSIFYRPKGREDEADGVREKFQR-PESDH 1010
            .:|..|::|...:|::......|...||.|.:.||....|..|.|:|..||..|::..: ..|||
Human   692 HLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKDTRSDH 756

  Fly  1011 LTYLNVYQQW-----RQNNYSSTWCNEHFIHIKAMRKVREVRQQLKD------IMTQQNLSVISC 1064
            ||.:|.::.|     |...|...:|.|:|:....::.:..::.|..:      .::.:|......
Human   757 LTVVNAFEGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPKDPES 821

  Fly  1065 GIDWD---IVRKCICSAYFYQAARLK-GIGE-----YVNLRTGMPCHLHPTSALYGLGTTP---D 1117
            .|:.|   |::..||:..:.:.|::: .:|:     .|..:|.....:||.|.  .:..|.   :
Human   822 NINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTKTDGLVAVHPKSV--NVEQTDFHYN 884

  Fly  1118 YVVYHELIMTAKEYMQCATAVDGYWLAELGPMFFSVKESGRSGREKKKQAAE------------H 1170
            :::||..:.|:..|:...|.|..|.|     :||....|.:...:::..|.:            |
Human   885 WLIYHLKMRTSSIYLYDCTEVSPYCL-----LFFGGDISIQKDNDQETIAVDEWIVFQSPARIAH 944

  Fly  1171 L-----KEME---EQMLKAQHEMEERKQQAAEREEQLATKQEIATPGNATPRRTPAR 1219
            |     ||::   ::.:::.|.::....::.:.....|....|.|...||||..|.|
Human   945 LVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKTQEKATPRNFPPR 1001

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Prp16	NP_572947.1	HrpA	534..>1148	CDD:441249	219/715 (31%)
DHX36	NP_065916.2	Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000269|PubMed:18279852	1..200		61/346 (18%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000250|UniProtKB:D4A2Z8	1..104		38/186 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..58		25/105 (24%)
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000269|PubMed:18854321	1..51		21/86 (24%)
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000269|PubMed:21149580, ECO:0000269|PubMed:21846770, ECO:0000269|PubMed:24151078	53..105		16/98 (16%)
		DSM (DHX36-specific motif). /evidence=ECO:0000269|PubMed:20472641, ECO:0000269|PubMed:22238380, ECO:0000269|PubMed:24369427, ECO:0000269|PubMed:26195789, ECO:0000269|PubMed:26649896	53..75		5/46 (11%)
		RecA-like domain 1. /evidence=ECO:0000250|UniProtKB:Q05B79	106..386		100/344 (29%)
		DEXHc_DHX36	207..386	CDD:350739	77/179 (43%)
		HrpA	215..>776	CDD:441249	190/566 (34%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	265..317		19/51 (37%)
		DEAH box. /evidence=ECO:0000255|PROSITE-ProRule:PRU00541	334..337		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250|UniProtKB:Q05B79	387..628		64/245 (26%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	498..557		18/60 (30%)
		Nuclear localization signal. /evidence=ECO:0000303|PubMed:14731398	517..528		3/10 (30%)
		WH domain. /evidence=ECO:0000250|UniProtKB:Q05B79	629..698		26/68 (38%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	638..697		20/58 (34%)
		OB_NTP_bind	831..917	CDD:400182	20/92 (22%)
		OB-fold-like subdomains. /evidence=ECO:0000250|UniProtKB:Q05B79	841..905		13/65 (20%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	849..860		1/10 (10%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	870..900		8/31 (26%)