DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNAlig4 and lig4

DIOPT Version :9

Sequence 1:	NP_572907.1	Gene:	DNAlig4 / 32322	FlyBaseID:	FBgn0030506	Length:	918	Species:	Drosophila melanogaster
Sequence 2:	NP_001096593.1	Gene:	lig4 / 569525	ZFINID:	ZDB-GENE-070820-10	Length:	909	Species:	Danio rerio

Alignment Length:	960	Identity:	288/960 - (30%)
Similarity:	462/960 - (48%)	Gaps:	130/960 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 IASTIKFRDICSLFEKIKATQKVANKEEVLKSYYESFCRHRESFRRQTGLNNDQPEDGASSFYSV 69
            :|:.:.|..:|::.|||:.|:...:|.::||.:.:|:    .:|  ...|:.|.| |.|.|||:.
Zfish    15 VAAQVPFIHLCTVLEKIQKTKLRPDKSKILKEFIDSW----RNF--HAALHKDNP-DTADSFYAA 72

  Fly    70 LRLLLPGADTGRDTYGLQITALGRLYIRVLQLPTDSSDAIRLQHRNGNMYR----------DYGD 124
            :||::|..:..|..||::...|.:|||.||.||.:..:|.:|.:     ||          |:..
Zfish    73 MRLIVPSFERERMAYGIKENMLAKLYIEVLGLPKNGDEANKLLN-----YRAPTTSQGEAGDFAL 132

  Fly   125 VVYSVLKPRCFNPPSNLRLKEIHQMLDTIANEDTEVKQQQ----LIRFTEQASPEEQKWLIRLLL 185
            :.|.|||.|| ....||.:||::..|.::|..:...::.|    |:....|:|..|||||||::|
Zfish   133 MAYFVLKKRC-TSQGNLSIKEVNDFLGSVATNNASKQKDQVKKSLLHLITQSSALEQKWLIRMIL 196

  Fly   186 KSLGLGIGEQKIFGVLHPKAQDIYQRCSDLGHVCNLLADRTTDLDASSSKDSKAAVKFVNLNSVI 250
            |.:.|||.::.:..|.||.|.:.|...:||..||..|.|.:..|...|                |
Zfish   197 KDMKLGISKETVLQVFHPDAPEFYNVTTDLNKVCRQLHDPSVSLSEVS----------------I 245

  Fly   251 RPFHQIRPMLCE----RFPGDIQELMQSDVLYLETKMDGERFQLHIDRGRFMYISRNGVDYTRNF 311
            ..|...:|||..    |   .|::.|.:...|:|||:||||.|||.....:.|.:||..:||:.|
Zfish   246 DLFSAFKPMLAAVANIR---QIEKQMGNRPFYIETKLDGERIQLHKAGDVYKYYTRNSYEYTQQF 307

  Fly   312 GHSYDHGTLTPKLRGLLPLGLESIILDGEMMVWDTNKLRFREKGENTDVKSLKPEGSWQPCFVVY 376
            |.|...|:|||.:..:....:.:.|||||||.::.....|.:||...|:|.|..:...|.||.|:
Zfish   308 GASPLEGSLTPYIHTVFSPHVVNCILDGEMMAYNPKTETFMQKGSKFDIKRLVDDSDLQTCFCVF 372

  Fly   377 DLLYFNGQSLLDHTYIQRAYKLQKLIVEQSGVLQLMRARKIGSVQEFNELFQQALDSHAEGIVLK 441
            |:|..|.|........:|...|:.:.....|.:.|:...:..::||......:|:|:..|||::|
Zfish   373 DVLLINNQKFGKEPLKKRYESLETVFSPIKGRIHLVSKSEGRTMQEVVNSLNEAIDNREEGIMVK 437

  Fly   442 KQGSRYQPGVRLGGGWYKDKADYIKGLITEFDVLIIGAFY--NRKRTFVDSFLLGVLQPAPPGSS 504
            ...|.|:|..| |.||.|.|.:|:.||:.|.|:||:|.::  .|:...:..||..|.:...||
Zfish   438 DPMSIYKPDKR-GEGWLKIKPEYVDGLMDELDLLIVGGYWGKGRRSGMLSHFLCAVAEVPSPG-- 499

  Fly   505 NRPEVF------SIGVVANNTKQRGVLNHTLKPHWHDV-VNEPPPLWFHYKPKERSGCPDLWIEP 562
            .:|.||      ..|.........||   .|..||... .|.||........|     |:::|||
Zfish   500 EKPTVFHTLCRIGSGYTLKELYDLGV---KLAKHWKVYNKNNPPAAILCGTEK-----PEVYIEP 556

  Fly   563 QNSVILQVKAADLAPNGAFFTRKSLHFPRTEMKRDDKTWSECMTLKEFNDLCGGPLAIKKLNKRQ 627
            :||||||||||::..:..:.|..:|.|||.|..|:||.|.:||||.:.|..  ...|..||..|.
Zfish   557 RNSVILQVKAAEIVTSEMYKTDCTLRFPRIERIREDKEWHQCMTLDDLNQF--RSKASGKLASRH 619

  Fly   628 LRLEDVTTKRKQMRMTPSERSRLGLAVYEKRYDASTSASTSKLFDGLSFCILSGSAGRHSKHQLQ 692
            |.:.:...::|:.::....:..:|:..:.|..|.|..|..:.:|:.:.||:::|. ..|||.:|:
Zfish   620 LNIHEDQPEKKKRKIVAKPKKTIGVIDHFKSQDLSKVAKETDMFEEVEFCVINGD-DEHSKAELE 683

  Fly   693 ELAVKNGGCIVENPLPNDPKCFCIAGDETFLVKRLILQQPRTCDIVRMEWLLRVCQ-KQELELKP 756
            :...:.||.:|:|| ..|..| .||..:...||.||.....  |:|...|||...: ||.:..:|
Zfish   684 KGVARCGGIVVQNP-GKDTYC-AIAAVQNMRVKNLISSDQH--DVVWATWLLECLENKQVIPWQP 744

  Fly   757 RDLIAATEPLQQDLAECFDRHGDSYTKDIANVEELQDLLQGI---ELTADNVAGITASNLNALED 818
            |.:|..:...::..|:.:|::||||..|.:. ::|:|:::.|   |:.:.:||.|.|.  ||.:|
Zfish   745 RHMIHMSPSTREHFAKEYDQYGDSYYVDTSE-QQLRDVIERISSAEVKSLSVAQIEAE--NAWDD 806

  Fly   819 QLLDGKKNLNMFRNLNAFF--YSPHGD----------EVAKLLFLQNGGRIVDDSDPQLNLGFIC 871
                  ...::||..:|:|  .:..||          :...|.|..:||::|:    :|..|
Zfish   807 ------LPTSIFRPYSAYFDRCADIGDPKSVICGTCLDTRALEFRFHGGKVVE----KLKEG--- 858

  Fly   872 MSSDIDNDHFEHWLHNHSKLTTD------------KVLNSAWIHQCHREG 909
                     ..|.:....|...|            |:::.:|:....:.|
Zfish   859 ---------ISHVVVEDMKRALDLKTLRRLHAKKFKIVHESWVTDSIKAG 899

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNAlig4	NP_572907.1	DNA_ligase_A_N	10..197	CDD:282522	66/200 (33%)
		dnl1	84..613	CDD:273147	185/555 (33%)
		Adenylation_DNA_ligase_IV	249..466	CDD:185713	74/220 (34%)
		OBF_DNA_ligase_IV	469..607	CDD:153437	50/146 (34%)
		BRCT	668..745	CDD:214602	25/76 (33%)
lig4	NP_001096593.1	DNA_ligase_A_N	20..208	CDD:282522	66/200 (33%)
		dnl1	86..606	CDD:273147	184/555 (33%)
		Adenylation_DNA_ligase_IV	240..461	CDD:185713	76/240 (32%)
		OBF_DNA_ligase_IV	464..601	CDD:153437	50/146 (34%)
		DNA_ligase_IV	754..786	CDD:288294	9/32 (28%)
		BRCT	<845..896	CDD:278934	10/66 (15%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170596805
Domainoid	1	1.000	116	1.000	Domainoid score	I5934
eggNOG	1	0.900	-	-		E1_COG1793
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1736
Inparanoid	1	1.050	381	1.000	Inparanoid score	I2023
OMA	1	1.010	-	-		QHG54896
OrthoDB	1	1.010	-	-		D189866at33208
OrthoFinder	1	1.000	-	-		FOG0004767
OrthoInspector	1	1.000	-	-		oto41409
orthoMCL	1	0.900	-	-		OOG6_103257
Panther	1	1.100	-	-	LDO	PTHR45997
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R326
SonicParanoid	1	1.000	-	-		X3920
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	17	16.800