DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNAlig4 and Lig4

DIOPT Version :9

Sequence 1:	NP_572907.1	Gene:	DNAlig4 / 32322	FlyBaseID:	FBgn0030506	Length:	918	Species:	Drosophila melanogaster
Sequence 2:	NP_001363971.1	Gene:	Lig4 / 319583	MGIID:	1335098	Length:	911	Species:	Mus musculus

Alignment Length:	966	Identity:	296/966 - (30%)
Similarity:	478/966 - (49%)	Gaps:	125/966 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 SVDIASTIKFRDICSLFEKIKATQKVANKEEVLKSYYESFCRHRESFRRQTGLNNDQPEDGASSF 66
            |..:|:.:.|.|:||..|:|:..:..|.|....|.:.:|:.:..::..:..       :|...||
Mouse     7 SQTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNR-------KDVTDSF 64

  Fly    67 YSVLRLLLPGADTGRDTYGLQITALGRLYIRVLQLPTDSSDAIR-LQHRNGNMYR-DYGD---VV 126
            |..:||:||..:..|..||::.|.|.:|||.:|.||.:..||.: |.:|..:..| |.||   :.
Mouse    65 YPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGARTDAGDFAMIA 129

  Fly   127 YSVLKPRCFNPPSNLRLKEIHQMLDTIANEDT----EVKQQQLIRFTEQASPEEQKWLIRLLLKS 187
            |.||||||....| |.:::::::||.:|:.::    ::.::.|::...|:|..|||||||:::|.
Mouse   130 YFVLKPRCLQKGS-LTIQQVNELLDLVASNNSGKKKDLVKKSLLQLITQSSALEQKWLIRMIIKD 193

  Fly   188 LGLGIGEQKIFGVLHPKAQDIYQRCSDLGHVCNLLADRTTDLDASSSKDSKAAVKFVNLNSVIRP 252
            |.|||.:|.||.:.|..|.:::...:||..||..|.|.:..|...|                |..
Mouse   194 LKLGISQQTIFSIFHNDAVELHNVTTDLEKVCRQLHDPSVGLSDIS----------------ITL 242

  Fly   253 FHQIRPMLC-----ERFPGDIQELMQSDVLYLETKMDGERFQLHIDRGRFMYISRNGVDYTRNFG 312
            |...:|||.     ||    :::.|:....|:|||:||||.|:|.|...:.|.||||.:||..||
Mouse   243 FSAFKPMLAAVADVER----VEKDMKQQSFYIETKLDGERMQMHKDGALYRYFSRNGYNYTDQFG 303

  Fly   313 HSYDHGTLTPKLRGLLPLGLESIILDGEMMVWDTNKLRFREKGENTDVKSLKPEGSWQPCFVVYD 377
            .|...|:|||.:.......:::.|||||||.::.....|.:||...|:|.:..:...|.|:.|:|
Mouse   304 ESPQEGSLTPFIHNAFGTDVQACILDGEMMAYNPTTQTFMQKGVKFDIKRMVEDSGLQTCYSVFD 368

  Fly   378 LLYFNGQSLLDHTYIQRAYKLQKLIVEQSGVLQLMRARKIGSVQEFNELFQQALDSHAEGIVLKK 442
            :|..|.:.|...|..:|...|........|.:::::..:..:.:|..:....|:|...|||::|.
Mouse   369 VLMVNKKKLGRETLRKRYEILSSTFTPIQGRIEIVQKTQAHTKKEVVDALNDAIDKREEGIMVKH 433

  Fly   443 QGSRYQPGVRLGGGWYKDKADYIKGLITEFDVLIIGAFYNR--KRTFVDSFLLGVLQPAPPGSSN 505
            ..|.|:|..| |.||.|.|.:|:.||:.|.||||:|.::.:  :...:..||..|.:..|||  :
Mouse   434 PLSIYKPDKR-GEGWLKIKPEYVSGLMDELDVLIVGGYWGKGSRGGMMSHFLCAVAETPPPG--D 495

  Fly   506 RPEVFSIGVVANNTKQRGVLNHTLKPHWHDVVNEPPPLW--FHYK-PKERSGC----PDLWIEPQ 563
            ||.||       :|..|....:|:| ..:|:..:....|  ||.| |.....|    |:::||||
Mouse   496 RPSVF-------HTLCRVGSGYTMK-ELYDLGLKLAKYWKPFHKKSPPSSILCGTEKPEVYIEPQ 552

  Fly   564 NSVILQVKAADLAPNGAFFTRKSLHFPRTEMKRDDKTWSECMTLKEFNDLCGGPLAIKKLNKRQL 628
            ||||:|:|||::.|:..:.|..:|.|||.|..||||.|.|||||.:...|.|  .|..||..:.|
Mouse   553 NSVIVQIKAAEIVPSDMYKTGSTLRFPRIEKIRDDKEWHECMTLGDLEQLRG--KASGKLATKHL 615

  Fly   629 RLEDVTTKRKQMRMTPSERSRLGLAVYE--KRYDASTSASTSKLFDGLSFCILSGSAGRHSKHQL 691
            .:.|....|:: |..|..:::..:.:.|  |..:.|.....|.:|:.:.||::||..| :.|..|
Mouse   616 HVGDDDEPREK-RRKPISKTKKAIRIIEHLKAPNLSNVNKVSNVFEDVEFCVMSGLDG-YPKADL 678

  Fly   692 QELAVKNGGCIVENPLPNDPKCFC-IAGDETFLVKRLILQQPRTCDIVRMEWLLRVCQKQE--LE 753
            :....:.||.||:||   .|..:| |||.|...||.:|.....  |:|:.||||. |.|.:  :.
Mouse   679 ENRIAEFGGYIVQNP---GPDTYCVIAGSENVRVKNIISSDKN--DVVKPEWLLE-CFKTKTCVP 737

  Fly   754 LKPRDLIAATEPLQQDLAECFDRHGDSYTKDIANVEELQDLLQGI----ELTADNVAGITASNLN 814
            .:||.:|......:|..|..:|.:||||..| .::::|:::..||    :.|.:.:|.:.|.
Mouse   738 WQPRFMIHMCPSTKQHFAREYDCYGDSYFVD-TDLDQLKEVFLGIKPSEQQTPEEMAPVIAD--- 798

  Fly   815 ALEDQLLDGKKNLNMFRNLNAF--FYSPHGD-----EVAKL----LFLQ-NGGRIVDDSDPQLNL 867
             ||.:.......|:|||:...:  .|:...|     |..:|    |.|: :|.::|.
Mouse   799 -LECRYSWDHSPLSMFRHYTIYLDLYAVINDLSSRIEATRLGITALELRFHGAKVVS-------- 854

  Fly   868 GFICMSSDIDNDHFEHWLHNHSKLTTD------------KVLNSAWIHQCHREGIL 911
               |:|..:.:    ..:....:..||            |:|..:|:.....:|.|
Mouse   855 ---CLSEGVSH----VIIGEDQRRVTDFKIFRRMLKKKFKILQESWVSDSVDKGEL 903

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNAlig4	NP_572907.1	DNA_ligase_A_N	10..197	CDD:282522	65/195 (33%)
		dnl1	84..613	CDD:273147	190/551 (34%)
		Adenylation_DNA_ligase_IV	249..466	CDD:185713	73/221 (33%)
		OBF_DNA_ligase_IV	469..607	CDD:153437	55/146 (38%)
		BRCT	668..745	CDD:214602	29/77 (38%)
Lig4	NP_001363971.1	dnl1	81..602	CDD:273147	190/552 (34%)
		BRCT_DNA_ligase_IV_rpt1	658..745	CDD:349354	33/93 (35%)
		DNA_ligase_IV	750..782	CDD:371515	10/32 (31%)
		BRCT_DNA_ligase_IV_rpt2	813..900	CDD:349349	17/101 (17%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850782
Domainoid	1	1.000	121	1.000	Domainoid score	I5713
eggNOG	1	0.900	-	-		E1_COG1793
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1736
Inparanoid	1	1.050	388	1.000	Inparanoid score	I1998
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54896
OrthoDB	1	1.010	-	-		D274264at2759
OrthoFinder	1	1.000	-	-		FOG0004767
OrthoInspector	1	1.000	-	-		oto93979
orthoMCL	1	0.900	-	-		OOG6_103257
Panther	1	1.100	-	-	LDO	PTHR45997
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R326
SonicParanoid	1	1.000	-	-		X3920
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	16	15.800