DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNAlig4 and Lig4

DIOPT Version :9

Sequence 1:	NP_572907.1	Gene:	DNAlig4 / 32322	FlyBaseID:	FBgn0030506	Length:	918	Species:	Drosophila melanogaster
Sequence 2:	NP_001099565.1	Gene:	Lig4 / 290907	RGDID:	1304639	Length:	911	Species:	Rattus norvegicus

Alignment Length:	879	Identity:	271/879 - (30%)
Similarity:	445/879 - (50%)	Gaps:	90/879 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 IASTIKFRDICSLFEKIKATQKVANKEEVLKSYYESFCRHRESFRRQTGLNNDQPEDGASSFYSV 69
            :|:.:.|.|:||..|:|:..:..|.|....|.:.:|:.:..::..:.   ..|.|:    |||..
  Rat    10 VAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKN---KKDVPD----SFYPA 67

  Fly    70 LRLLLPGADTGRDTYGLQITALGRLYIRVLQLPTDSSDAIR-LQHRNGNMYR----DYGDVVYSV 129
            :||:||..:..|..||::.|.|.:|||.:|.||.:..||.: |.:|..:..|    |:..:.|.|
  Rat    68 MRLILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGARSDAGDFATIAYFV 132

  Fly   130 LKPRCFNPPSNLRLKEIHQMLDTIANEDT----EVKQQQLIRFTEQASPEEQKWLIRLLLKSLGL 190
            |||||....| |.:::::::||.:|:.::    ::.::.|::...|.|..|||||||:::|.|.|
  Rat   133 LKPRCLQKGS-LTIQKVNELLDLVASNNSGKRKDLVKKSLLQLITQCSALEQKWLIRMIIKDLKL 196

  Fly   191 GIGEQKIFGVLHPKAQDIYQRCSDLGHVCNLLADRTTDLDASSSKDSKAAVKFVNLNSVIRPFHQ 255
            |:.:..|..:.|..|.:::...:||..||..|.|.:..|...|                |..|..
  Rat   197 GVSQHTILSIFHNDAVELHNVTTDLEKVCRQLHDPSVGLSDIS----------------ITLFSA 245

  Fly   256 IRPMLC-----ERFPGDIQELMQSDVLYLETKMDGERFQLHIDRGRFMYISRNGVDYTRNFGHSY 315
            .||||.     ||    :::.|:....|:|||:||||.|:|.|...:.|.||||.:||..||.|.
  Rat   246 FRPMLAAVADVER----VEKDMKQQSFYIETKLDGERMQMHKDGALYRYFSRNGYNYTDQFGESP 306

  Fly   316 DHGTLTPKLRGLLPLGLESIILDGEMMVWDTNKLRFREKGENTDVKSLKPEGSWQPCFVVYDLLY 380
            ..|:|||.:.......::..|||||||.::.....|.:||...|:|.:..:...|.|:.|:|:|.
  Rat   307 QEGSLTPFIHNAFKTDVQVCILDGEMMAYNPTTQTFMQKGVKFDIKRMVEDSDLQTCYCVFDVLM 371

  Fly   381 FNGQSLLDHTYIQRAYKLQKLIVEQSGVLQLMRARKIGSVQEFNELFQQALDSHAEGIVLKKQGS 445
            .|.:.|...|..:|...|........|.:::::..:..:.||..:....|:|...|||::|...|
  Rat   372 VNNKKLGRETLRKRYDILSSTFTPIQGRIEIVQKTQAQTKQEVVDALNDAIDKREEGIMVKHPLS 436

  Fly   446 RYQPGVRLGGGWYKDKADYIKGLITEFDVLIIGAFYNR--KRTFVDSFLLGVLQPAPPGSSNRPE 508
            .|:|..| |.||.|.|.:|:.||:.|.|:||:|.::.:  :...:..||..|.:..|||  ::|.
  Rat   437 IYKPDKR-GEGWLKIKPEYVTGLMDELDLLIVGGYWGKGSRGGMMSHFLCAVAETPPPG--DKPS 498

  Fly   509 VFSIGVVANNTKQRGVLNHTLKPHWHDVVNEPPPLWFHYKPKERSGC-------PDLWIEPQNSV 566
            ||       :|..|....:|:| ..:|:..:....|..::.|....|       |:::|||||||
  Rat   499 VF-------HTLCRVGSGYTMK-ELYDLGLKLAKYWKPFQRKSPPSCILCGTEKPEVYIEPQNSV 555

  Fly   567 ILQVKAADLAPNGAFFTRKSLHFPRTEMKRDDKTWSECMTLKEFNDLCG---GPLAIKKLNKRQL 628
            |:|:|||::.|:..:.|..:|.|||.|..||||.|.:||||.:...|.|   |.||.|.|:..
  Rat   556 IVQIKAAEIVPSDMYKTGSTLRFPRIEKIRDDKEWHDCMTLDDLEQLRGKASGKLATKHLHIG-- 618

  Fly   629 RLEDVTTKRKQMRMTPSERSRLGLAVYEKRYDASTSASTSKLFDGLSFCILSGSAGRHSKHQLQE 693
              :|...:.|:.:.....:..:|:..:.|..:.|.....|.:|:.:.||::||..| :.|..|:.
  Rat   619 --DDDEPREKRRKPISKTKKTIGIIEHLKAPNLSNINKVSNVFEDVEFCVMSGLDG-YPKPDLEN 680

  Fly   694 LAVKNGGCIVENPLPNDPKCFC-IAGDETFLVKRLILQQPRTCDIVRMEWLLRVCQKQE--LELK 755
            ...:.||.||:||   .|..:| |||.|...|:.:|.....  |:|:.||||. |.|.:  :..:
  Rat   681 KIAEFGGYIVQNP---GPDTYCVIAGSENIRVRNIISSDRH--DVVKPEWLLE-CFKTKTCVPWQ 739

  Fly   756 PRDLIAATEPLQQDLAECFDRHGDSYTKDIANVEELQDLLQGI----ELTADNVAGITASNLNAL 816
            ||.:|......:|..|..:|.:||||..| |::::|:::...|    :.|.:.:|.:.|.    |
  Rat   740 PRFMIHMCPSTKQHFAREYDCYGDSYFVD-ADLDQLKEVFLRIKPSEQQTPEEMAPVIAD----L 799

  Fly   817 EDQLLDGKKNLNMFRNLNAF--FYSPHGDEVAKL 848
            |.:.......|:|||:...:  .|:...|..:|:
  Rat   800 ECRYSWDHSPLSMFRHYTVYLDLYAVINDLSSKI 833

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNAlig4	NP_572907.1	DNA_ligase_A_N	10..197	CDD:282522	62/195 (32%)
		dnl1	84..613	CDD:273147	181/551 (33%)
		Adenylation_DNA_ligase_IV	249..466	CDD:185713	75/221 (34%)
		OBF_DNA_ligase_IV	469..607	CDD:153437	49/146 (34%)
		BRCT	668..745	CDD:214602	28/77 (36%)
Lig4	NP_001099565.1	DNA_ligase_A_N	15..203	CDD:282522	62/195 (32%)
		dnl1	81..602	CDD:273147	181/552 (33%)
		Adenylation_DNA_ligase_IV	235..455	CDD:185713	76/240 (32%)
		OBF_DNA_ligase_IV	459..596	CDD:153437	49/146 (34%)
		BRCT	654..730	CDD:278934	29/82 (35%)
		DNA_ligase_IV	750..782	CDD:288294	10/32 (31%)
		BRCT	<845..899	CDD:237994
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166354488
Domainoid	1	1.000	122	1.000	Domainoid score	I5520
eggNOG	1	0.900	-	-		E1_COG1793
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1736
Inparanoid	1	1.050	384	1.000	Inparanoid score	I1967
OMA	1	1.010	-	-		QHG54896
OrthoDB	1	1.010	-	-		D189866at33208
OrthoFinder	1	1.000	-	-		FOG0004767
OrthoInspector	1	1.000	-	-		oto97509
orthoMCL	1	0.900	-	-		OOG6_103257
Panther	1	1.100	-	-	LDO	PTHR45997
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3920
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.770