DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tomosyn and STXBP5L

DIOPT Version :9

Sequence 1:	NP_001162735.1	Gene:	Tomosyn / 32217	FlyBaseID:	FBgn0030412	Length:	1470	Species:	Drosophila melanogaster
Sequence 2:	NP_001335272.1	Gene:	STXBP5L / 9515	HGNCID:	30757	Length:	1186	Species:	Homo sapiens

Alignment Length:	1519	Identity:	537/1519 - (35%)
Similarity:	760/1519 - (50%)	Gaps:	384/1519 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKKFTFKGVLDGFRQ--------------------SVQPQATR--QEQEIQEQLKADHFTLKKTF 43
            ||||.|:.||||...                    ||.|..|.  ..:||||.|.:::|.:.||.
Human     1 MKKFNFRKVLDGLTASSPGSGSSSGSNSGGGAGSGSVHPAGTAGVLREEIQETLTSEYFQICKTV 65

  Fly    44 RHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVT 108
            |||||:.||:.|||||||:||||.::|.||||||||||.:.:|  ||..|||..|||:||||||:
Human    66 RHGFPHQPTALAFDPVQKILAIGTRTGAIRILGRPGVDCYCQH--ESGAAVLQLQFLINEGALVS 128

  Fly   109 VTADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYI 173
            .::|||:|||::|||.|.|:.||||.|||:|..|||..|||||||||:||.|:|:|::|.||||:
Human   129 ASSDDTLHLWNLRQKRPAILHSLKFNRERITYCHLPFQSKWLYVGTERGNTHIVNIESFILSGYV 193

  Fly   174 INWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAW 238
            |.||||||:...:|||.|:.|.|:|.|..||||.:|.|.:|.||||:|.||||....||:.|:.|
Human   194 IMWNKAIELSTKTHPGPVVHLSDSPRDEGKLLIGYENGTVVFWDLKSKRAELRVYYDEAIHSIDW 258

  Fly   239 HYEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDG-NAEKCKPIYKVDLKSSATGETFT 302
            |:|||.|:.||:|||:..|..|..::|.....||.|..::| .:|.||||.||:.|:....|.|.
Human   259 HHEGKQFMCSHSDGSLTLWNLKSPSRPFQTTIPHGKSQREGRKSESCKPILKVEYKTCKNSEPFI 323

  Fly   303 IFSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVL 367
            |||||:..:|..:...:|:|.|||.|||||:|.:.:|:||||.|:|.|.|||||:.|||:.||::
Human   324 IFSGGLSYDKACRRPSLTIMHGKAITVLEMDHPIVEFLTLCETPYPNEFQEPYAVVVLLEKDLIV 388

  Fly   368 IDLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGG 432
            :||....||.||:|||||:|||||||..|..|||.||:...||:|  ...||..:|.:|||||||
Human   389 VDLTQSNFPIFENPYPMDIHESPVTCTAYFADCPPDLILVLYSIG--VKHKKQGYSNKEWPISGG 451

  Fly   433 EWSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPE--SDNPLA 495
            .|:..:.:|.||:||||.|||:||||:.|.|||:||||||:|:|||.:........|  .::|.|
Human   452 AWNLGAQTYPEIIITGHADGSIKFWDASAITLQMLYKLKTSKVFEKQKVGEGKQTCEIVEEDPFA 516

  Fly   496 VHLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYE-------------NFDDIY 547
            :.:|:.|.|||..||:|....|:::||.:.|.|:|::.||:.:.|:             .|.|:.
Human   517 IQMIYWCPESRIFCVSGVSAYVIIYKFSRHEITTEIVSLEVRLQYDVEDIITPEPETSPPFPDLS 581

  Fly   548 GTSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVC-LTTGPNRRNVQVTSL 611
            ...|....|:|.....|....:..:..| |.|:....|.|||:|::||. |.........|:|||
Human   582 AQLPSSRSLSGSTNTVASEGVTKDSIPC-LNVKTRPVRMPPGYQAELVIQLVWVDGEPPQQITSL 645

  Fly   612 CISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSS 676
            .:||:||::|:|...||.::|.|||..|||:...|||.:.|.|.|.|:||::  ||
Human   646 AVSSAYGIVAFGNCNGLAVVDFIQKTVLLSMGTIDLYRSSDLYQRQPRSPRK--NK--------- 699

  Fly   677 DQLNDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAAS 741
                          :|:.:
Human   700 --------------QFIAD---------------------------------------------- 704

  Fly   742 AAVMMAKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFYTP 806
                                                             |..|..|.|   ||
Human   705 -------------------------------------------------NFCMRGLSN---FY-- 715

  Fly   807 KEVSPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTD------- 864
                |:......|..::..|      |:|             ||.|....:.:|.|:|
Human   716 ----PDLTKRIRTSYQSLTE------LND-------------SPVPLELERCKSPTSDHVNGHCT 757

  Fly   865 SPDEGASSSIASGIRRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKF-KEKSRDQRLLSVPNI 928
            ||   .|.|.:||.|.      :..:|.:.|.         ..|.|..| |.:|.....:|:|..
Human   758 SP---TSQSCSSGKRL------SSADVSKVNR---------WGPGRPPFRKAQSAACMEISLPVT 804

  Fly   929 KYQPRDSRGGARNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSS 993
            ..:.|::                                              :::||||||:||
Human   805 TEENREN----------------------------------------------SYNRSRSSSISS 823

  Fly   994 IDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRK-SQPVLITING 1057
            ||..|.|::|.|.|::|:|::|| .|:.|.|::|||.|.:|.:.:.:|..|.:: ::||:: :..
Human   824 IDKDSKEAITALYFMDSFARKND-STISPCLFVGTSLGMVLIISLNLPLADEQRFTEPVMV-LPS 886

  Fly  1058 GPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTS 1122
            |..:.|||::.:.|.:|..|.::...:|.|||....:::.   ||..|...
Human   887 GTFLSLKGAVLTFSCMDRMGGLMQPPYEVWRDPNNIDENE---KSWRRKVV-------------- 934

  Fly  1123 VAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASS 1187
                                                                     |:|||||
Human   935 ---------------------------------------------------------MNSSSAS- 941

  Fly  1188 SGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLA 1252
                       :.:.|.||.:|.||||.|||.:.:|.|:....::|..|.::|..:.|...:|||
Human   942 -----------QEIGDHQYTIICSEKQAKVFSLPSQTCLYVHNITETSFILQANVVVMCSSACLA 995

  Fly  1253 TYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIF 1317
            .:.:|||:|:.|||||:.:||.::|||.::                             :|::.|
Human   996 CFCANGHIMIMSLPSLRPMLDVNYLPLTDM-----------------------------RIARTF 1031

  Fly  1318 CFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDR 1382
            ||:::|..||:.||||||:.|.|.|.|..:.:|:|:|:|..|.||....||.||||||..:..||
Human  1032 CFTNEGQALYLVSPTEIQRLTYSQEMCDNLQDMLGDLFTPIETPEAQNRGFLKGLFGGSGQTFDR 1096

  Fly  1383 EELFGEQS-GKPNRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMA 1445
            ||||||.| ||.:||:|:||||| ::|.:...|.....|::||.....|||::|..|||:...|.
Human  1097 EELFGEASAGKASRSLAQHIPGPGSIEGMKGAAGGVMGELTRARIALDERGQRLGELEEKTAGMM 1161

  Fly  1446 NTAQDFSGTAHQLMLKYKDKKWYQ 1469
            .:|:.||..||:||||||||||||
Human  1162 TSAEAFSKHAHELMLKYKDKKWYQ 1185

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_001162735.1	WD40	52..257	CDD:295369	124/204 (61%)
		WD40	<55..257	CDD:225201	123/201 (61%)
		WD40 repeat	95..133	CDD:293791	23/37 (62%)
		WD40 repeat	138..184	CDD:293791	30/45 (67%)
		WD40 repeat	192..226	CDD:293791	18/33 (55%)
		LLGL	269..375	CDD:285555	51/106 (48%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	24/60 (40%)
STXBP5L	NP_001335272.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	15..40		2/24 (8%)
		WD40	74..>281	CDD:421866	125/208 (60%)
		WD 1	74..107		23/32 (72%)
		WD40 repeat	74..107	CDD:293791	23/32 (72%)
		WD 2	114..153		24/38 (63%)
		WD40 repeat	115..153	CDD:293791	23/37 (62%)
		WD 3	158..194		23/35 (66%)
		WD40 repeat	211..248	CDD:293791	21/36 (58%)
		WD 4	213..247		19/33 (58%)
		WD40	249..>552	CDD:225201	148/304 (49%)
		WD 5	253..285		14/31 (45%)
		WD40 repeat	253..279	CDD:293791	13/25 (52%)
		LLGL	288..396	CDD:400597	51/107 (48%)
		WD 6	307..349		18/41 (44%)
		WD 7	357..391		18/33 (55%)
		WD 8	413..490		43/78 (55%)
		WD 9	518..629		32/111 (29%)
		WD 10	643..705		30/181 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	748..771		9/31 (29%)
		WD 11	832..889		20/58 (34%)
		WD 12	898..969		28/156 (18%)
		WD 13	974..1018		18/43 (42%)
		WD 14	1032..1055		12/22 (55%)
		R-SNARE_STXBP5	1119..1179	CDD:277246	23/59 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165158372
Domainoid	1	1.000	351	1.000	Domainoid score	I1019
eggNOG	1	0.900	-	-		E1_KOG1983
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1008	1.000	Inparanoid score	I288
Isobase	1	0.950	-	0.861654	Normalized mean entropy	S4092
OMA	1	1.010	-	-		QHG49198
OrthoDB	1	1.010	-	-		D53111at33208
OrthoFinder	1	1.000	-	-		FOG0000702
OrthoInspector	1	1.000	-	-		otm40406
orthoMCL	1	0.900	-	-		OOG6_104979
Panther	1	1.100	-	-	O	PTHR10241
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2301
SonicParanoid	1	1.000	-	-		X453
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.750