DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Tomosyn and SRO77

DIOPT Version :9

Sequence 1:	NP_001162735.1	Gene:	Tomosyn / 32217	FlyBaseID:	FBgn0030412	Length:	1470	Species:	Drosophila melanogaster
Sequence 2:	NP_009444.2	Gene:	SRO77 / 852168	SGDID:	S000000202	Length:	1010	Species:	Saccharomyces cerevisiae

Alignment Length:	692	Identity:	145/692 - (20%)
Similarity:	253/692 - (36%)	Gaps:	173/692 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 EIQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESE 91
            ::...:.:..|..||...:|.....|...||..|.|||:...:|.|.:.|:..::
Yeast    22 DVSNGINSKFFDTKKICTYGINGRITVTTFDYTQSLLAVATTAGEIHVYGQKQIE---------- 76

  Fly    92 CAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTPRIVQSLK--------FQRERVTCIHLPVGSK 148
              |:|.  |.|...:..:.....|:|.::.:|:..||.|:.        |....:|||.......
Yeast    77 --VVFT--LKNRPQIKHMRFIKGIYLIAVDEKSNIIVLSVHSKQILTTVFCPNSITCIETDPSLD 137

  Fly   149 WLYVGTEKGNIHVVHIDTFALSGY-IINWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGL 212
            |:.:|.|.|:|.:..:|...:|.. |.|:.|::.:.: .....||::..||.|...:||::| .:
Yeast   138 WMLIGLESGSILIYDVDRNQMSKLKIENFQKSVFLPK-ERLSPVISIQWNPRDIGTILISYE-HI 200

  Fly   213 LVLW----------------------DLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSIC 255
            .|::                      ||.....:.|  ..:.::|| :|....:.::.|.|.|:.
Yeast   201 TVIYSFIDYKVKQHFFYQLEPYAPGGDLSTNIEKKR--TPKVIQSL-YHPNSLHILTVHEDNSLV 262

  Fly   256 SWPTKPQAKPQSQVCPHAKIN------KDGNAEKCKPIYKVD-------------LKSSAT---- 297
            .|.........::......:|      ||.:..:...|:||.             :.:.||
Yeast   263 FWDVNSGKLIHARSIFETHVNFPNPALKDCSFTETPAIFKVSWLCQRNPEYTSLLIATKATENPC 327

  Fly   298 --GETFTIFSGGMPSEKGSKSNCIT------------VMVGKATTVLEMEHAVCDFITLCENPWP 348
              .|...|..||.|....:..:.::            .::|||..:               |..|
Yeast   328 LPQEITMIDLGGTPMYSVTSFDAMSKYYAKPVQQKLFSLIGKAPLI---------------NFLP 377

  Fly   349 CETQEPY-------AIAVLLQYDLVLIDLLTP--GFPCFESPYPMDLHESPVTCCTYLTDCPSDL 404
            .....||       .:.:||..|..|..|:.|  .|....|.:|..|.....|    :|.|.:..
Yeast   378 LPKASPYFGGCHDTNLILLLLEDGELETLIYPAGSFSSKASIFPRSLAWVRPT----VTTCIAQS 438

  Fly   405 VPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSY-----SEIVITGHQDGSLKFWDSGAG-- 462
            |.....:|..|.::...|      :.||  .||:.:.     ...::|||.:||::.||:...
Yeast   439 VQKNLWLGMMTIAQSESF------LKGG--IPATRNIRRHETRSALLTGHSNGSVRIWDASHSEV 495

  Fly   463 TLQILYKLKTAKMFEKPRSHVAHSHPESDNPLAVHLIFLCSESRRLCVAGAMGQVMLFKFRKVES 527
            |...::::.|||:..:..:            ||:..|...||:..|.|:..:|.|:||||..
Yeast   496 TDNAVFEVNTAKVLNRATN------------LAIKNISFASETLELAVSSEVGDVILFKFET--- 545

  Fly   528 TSEVLVLEIPILYENFDDIYGTSPECDFLTGHQVQKAE-SSDSDKTAECSLKVRNGAQRKPPGFQ 591
                            :..||..|:.|.|   |::.:. |.|..||....:..|.....|.....
Yeast   546 ----------------NKFYGQLPKSDAL---QLKFSRFSLDDSKTILVDVSDRGPTNVKQGFIP 591

  Fly   592 SQLVCLTTGPNRRNVQVTSLCISSSYGLMAYG-TEYGLVIID 632
            |.::....|       ..|..::|:.|.:|.| .|..|:|:|
Yeast   592 STVIHAKKG-------AVSAIMNSNIGFVAVGFIEGTLIILD 626

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_001162735.1	WD40	52..257	CDD:295369	52/235 (22%)
		WD40	<55..257	CDD:225201	51/232 (22%)
		WD40 repeat	95..133	CDD:293791	9/45 (20%)
		WD40 repeat	138..184	CDD:293791	13/46 (28%)
		WD40 repeat	192..226	CDD:293791	10/55 (18%)
		LLGL	269..375	CDD:285555	26/151 (17%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226
SRO77	NP_009444.2	WD40_like	<48..>151	CDD:407205	28/116 (24%)
		WD40 repeat	88..122	CDD:293791	6/33 (18%)
		WD40 repeat	127..167	CDD:293791	12/39 (31%)
		WD40 repeat	180..233	CDD:293791	11/53 (21%)
		WD40 repeat	240..264	CDD:293791	6/24 (25%)
		Lgl_C	514..916	CDD:400768	36/154 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157345886
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1983
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0.861654	Normalized mean entropy	S4092
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm46530
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10241
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2301
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	8	7.820