DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tomosyn and Stxbp5

DIOPT Version :9

Sequence 1:	NP_001162735.1	Gene:	Tomosyn / 32217	FlyBaseID:	FBgn0030412	Length:	1470	Species:	Drosophila melanogaster
Sequence 2:	NP_848035.1	Gene:	Stxbp5 / 81022	RGDID:	708517	Length:	1152	Species:	Rattus norvegicus

Alignment Length:	1516	Identity:	545/1516 - (35%)
Similarity:	764/1516 - (50%)	Gaps:	412/1516 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKKFTFKGVLDGF-----------RQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSF 54
            |:||..:.||||.           :|...|...| |.||||.|:::||.|.||.||||||.|::.
  Rat     1 MRKFNIRKVLDGLTAGSSSASQQQQQQQHPPGNR-EPEIQETLQSEHFQLCKTVRHGFPYQPSAL 64

  Fly    55 AFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWS 119
            |||||||:||:|.::|.:|:.|||||:.:.:|  :|..||:..|||:||||||:..||||:|||:
  Rat    65 AFDPVQKILAVGTQTGALRLFGRPGVECYCQH--DSGAAVIQLQFLINEGALVSALADDTLHLWN 127

  Fly   120 IRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVR 184
            :|||.|.::.||||.|||||..|||..|||||||||:||||:|::::|.||||:|.||||||:..
  Rat   128 LRQKRPAVLHSLKFCRERVTFCHLPFQSKWLYVGTERGNIHIVNVESFTLSGYVIMWNKAIELSS 192

  Fly   185 TSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSH 249
            .||||.|:.:.|||:|..||||.||.|.:||||||:|.|:.|:...||:.|:|||:|||.|:.||
  Rat   193 KSHPGPVVHISDNPMDEGKLLIGFESGTVVLWDLKSKKADYRYTYDEAIHSVAWHHEGKQFICSH 257

  Fly   250 TDGSICSWPTKPQAKPQSQVCPHAKINKDG-NAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKG 313
            :||::..|..:...||...:.||.|..||| ..|.||||.||:.|::.:||.|.|.|||:..:..
  Rat   258 SDGTLTIWNVRSPTKPVQTITPHGKQLKDGKKPEPCKPILKVEFKTTRSGEPFIILSGGLSYDTV 322

  Fly   314 SKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCF 378
            .:..|:|||.||:|.||||::::.||:||||.|:|.:.|||||:.|||:.|||||||...|:|.|
  Rat   323 GRRPCLTVMHGKSTAVLEMDYSIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVLIDLAQNGYPIF 387

  Fly   379 ESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSE 443
            |:|||:.:||||||||.|..|||.||:||.||||  ...|:..:|::||||:||.|...:.||.|
  Rat   388 ENPYPLSIHESPVTCCEYFADCPVDLIPALYSVG--ARQKRQGYSKKEWPINGGNWGLGAQSYPE 450

  Fly   444 IVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPE--SDNPLAVHLIFLCSESR 506
            |:||||.|||:||||:.|.|||:||||||:|:|||.|:.....:.:  .::|.|:.:|..|.|||
  Rat   451 IIITGHADGSIKFWDASAITLQVLYKLKTSKVFEKSRNKDDRQNTDIVDEDPYAIQIISWCPESR 515

  Fly   507 RLCVAGAMGQVMLFKFRKVESTSEVL-VLEIPILYENFDDI--------------YGTS------ 550
            .||:||....|::::|.|.|..:||: :||:.:||| .:|:              .|:|
  Rat   516 MLCIAGVSAHVIIYRFSKQEVVTEVIPMLEVRLLYE-INDVETPEGEQPPPLSTPVGSSTSQPIP 579

  Fly   551 PECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVC-LTTGPNRRNVQVTSLCIS 614
            |:     .|....:.|||..:.....|||:|...::.||:|::||. |.........|:|||.::
  Rat   580 PQ-----SHPSTSSSSSDGLRDNVPCLKVKNSPLKQSPGYQTELVIQLVWVGGEPPQQITSLALN 639

  Fly   615 SSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQL 679
            |||||:.:|...|:.::|.:||..||:::..:|||::|||.|.|:||::       ||.||...|
  Rat   640 SSYGLVVFGNSNGIAMVDYLQKAVLLNLSTIELYGSNDPYRREPRSPRK-------SRQPSGAGL 697

  Fly   680 NDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAASAAV 744
            .|.|..     ..|||.
  Rat   698 CDITEG-----TVVPED------------------------------------------------ 709

  Fly   745 MMAKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFYTPKEV 809
             ..|||....::|....|.:|                        ||..:|.:|..|
  Rat   710 -RCKSPTSGSSSPHNSDDEQK------------------------VNNFIEKVKTQS-------- 741

  Fly   810 SPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTDSPDEGASSSI 874
                                                          .||            |..:
  Rat   742 ----------------------------------------------RKF------------SKMV 748

  Fly   875 ASGIRRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQRLLSVPNIKYQPRDSRGGA 939
            ||.:.::                                      .|.||:|.
  Rat   749 ASDLAKM--------------------------------------SRKLSLPT------------ 763

  Fly   940 RNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSSIDMSSSESVTC 1004
             :||.|                              :...|.:||||||||::|||..|.|:::.
  Rat   764 -DLKPD------------------------------LDVKDNSFSRSRSSSVTSIDKESREAISA 797

  Fly  1005 LAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMP-ERDVRKSQPVLITINGGPVVRLKGSIT 1068
            |.|.|::.::.| .:..|.||:||:.|:...:.:.:| ..:.|..|||::: ..|.::||||:|.
  Rat   798 LHFCETFTRKAD-SSPSPCLWVGTTVGTAFVITLNLPLGPEQRLLQPVIVS-PSGTILRLKGAIL 860

  Fly  1069 SMSFLDSYGSIIPYTFETW--------RDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAG 1125
            .|:|||:.|.::|..:|.|        :|||...|.|.|.
  Rat   861 RMAFLDAAGCLMPPAYEPWTEHNVPEEKDEKEKLKKRRPV------------------------- 900

  Fly  1126 GASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGI 1190
                                                                    |.|.|||
  Rat   901 --------------------------------------------------------SVSPSSS-- 907

  Fly  1191 SGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYL 1255
                    :.:::.||.||.||||.||..:..|.|..:..::|..|.::.:.:.:.:..|||.:.
  Rat   908 --------QEISENQYAVICSEKQAKVISLPTQNCAYKQNITETSFVLRGDIVALSNSVCLACFC 964

  Fly  1256 SNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFS 1320
            :|||:|..|||||:.|||..:|||..:                             :|::.|||:
  Rat   965 ANGHIMTFSLPSLRPLLDVYYLPLTNM-----------------------------RIARTFCFA 1000

  Fly  1321 HKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREEL 1385
            :.|..||:.||||||:.|.|.|.|:.:.||:|||:|..|.||.|..|||||||||||:.||||||
  Rat  1001 NSGQALYLVSPTEIQRLTYSQETCENLQEMLGELFTPVETPEAPNRGFFKGLFGGGAQSLDREEL 1065

  Fly  1386 FGE-QSGKPNRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTA 1448
            ||| .|||.:||:|:||||| .:|.:...||....|::||.....|||:||:.||||...|.::|
  Rat  1066 FGESSSGKASRSLAQHIPGPGGIEGVKGAASGVVGELARARLALDERGQKLSDLEERTAAMMSSA 1130

  Fly  1449 QDFSGTAHQLMLKYKDKKWYQ 1469
            ..||..||::|||||||||||
  Rat  1131 DSFSKHAHEMMLKYKDKKWYQ 1151

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_001162735.1	WD40	52..257	CDD:295369	118/204 (58%)
		WD40	<55..257	CDD:225201	118/201 (59%)
		WD40 repeat	95..133	CDD:293791	22/37 (59%)
		WD40 repeat	138..184	CDD:293791	31/45 (69%)
		WD40 repeat	192..226	CDD:293791	19/33 (58%)
		LLGL	269..375	CDD:285555	55/106 (52%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	26/60 (43%)
Stxbp5	NP_848035.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	14..35		3/21 (14%)
		WD40	<47..275	CDD:225201	132/229 (58%)
		WD40	62..280	CDD:295369	121/219 (55%)
		WD 1	62..95		17/32 (53%)
		WD40 repeat	62..95	CDD:293791	17/32 (53%)
		WD 2	102..141		23/38 (61%)
		WD40 repeat	103..141	CDD:293791	22/37 (59%)
		WD40 repeat	146..192	CDD:293791	31/45 (69%)
		WD 3	146..182		24/35 (69%)
		WD40 repeat	200..236	CDD:293791	20/35 (57%)
		WD 4	201..235		19/33 (58%)
		WD40	237..>539	CDD:225201	153/303 (50%)
		WD40 repeat	241..278	CDD:293791	15/36 (42%)
		WD 5	241..273		13/31 (42%)
		LLGL	277..384	CDD:285555	55/106 (52%)
		WD 6	295..337		18/41 (44%)
		WD 7	345..379		21/33 (64%)
		WD 8	401..478		46/78 (59%)
		WD 9	506..620		36/119 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	557..596		7/43 (16%)
		WD 10	634..696		29/68 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	675..731		23/140 (16%)
		WD 11	795..852		16/58 (28%)
		WD 12	861..935		30/164 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	879..907		10/108 (9%)
		WD 13	940..984		17/43 (40%)
		WD 14	998..1021		12/22 (55%)
		R-SNARE_STXBP5	1085..1145	CDD:277246	25/59 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166352363
Domainoid	1	1.000	344	1.000	Domainoid score	I1024
eggNOG	1	0.900	-	-		E1_KOG1983
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H16402
Inparanoid	1	1.050	953	1.000	Inparanoid score	I317
OMA	1	1.010	-	-		QHG49198
OrthoDB	1	1.010	-	-		D53111at33208
OrthoFinder	1	1.000	-	-		FOG0000702
OrthoInspector	1	1.000	-	-		otm44543
orthoMCL	1	0.900	-	-		OOG6_104979
Panther	1	1.100	-	-	LDO	PTHR10241
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X453
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	16	15.770