Protein Alignment Tomosyn and stxbp5l

DIOPT Version :10

• Sequence 1: NP_727628.3
• Gene: Tomosyn / 32217
• FlyBaseID: FBgn0030412
• Length: 1470
• Species: Drosophila melanogaster
• Sequence 2: XP_005167782.1
• Gene: stxbp5l / 567605
• ZFINID: ZDB-GENE-041210-220
• Length: 1267
• Species: Danio rerio
• Alignment Length: 1527
• Identity: 558/1527 - (36%)
• Similarity: 779/1527 - (51%)
• Gaps: 319/1527 - (20%)

  Fly     1 MKKFTFKGVLDGFRQS--VQP---------------QATRQEQEIQEQLKADHFTLKKTFRHGFP 48
            |||  |:.||||...|  |.|               ..|.:|.||||.|.::||.:.||.|||||
Zfish     1 MKK--FRKVLDGLTTSSPVNPGGSPGCGSAAGTPSAAPTPRELEIQETLMSEHFQICKTVRHGFP 63

  Fly    49 YSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADD 113
            |.||:.|||||||:||||.:||.||||||||||.|::|  ||..|||..|||:|||||||..|||
Zfish    64 YQPTALAFDPVQKILAIGSRSGGIRILGRPGVDCHSQH--ESGAAVLQMQFLINEGALVTACADD 126

  Fly   114 TIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNK 178
            |:||||:||:.|.|:.||||.|||:|..|||..|||||||||:||.|:|:|::|.||||:|.|||
Zfish   127 TLHLWSLRQRLPAILHSLKFNRERITFCHLPFQSKWLYVGTERGNTHIVNIESFILSGYVIMWNK 191

  Fly   179 AIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGK 243
            |||:...:|||.|:.|.|:|.|..||||.||.|.:|:|||:||.|:.|....||:.|::||:||:
Zfish   192 AIELSTKTHPGPVVHLSDSPKDEGKLLIGFESGTIVMWDLRAKRADFRIYYDEAIHSVSWHHEGR 256

  Fly   244 YFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSA-TGETFTIFSGG 307
            .|:.||:|||:..|..:..|||.....||.|..:||..|.||||.||:.|:|. :.|.|.|||||
Zfish   257 QFMCSHSDGSLSMWNMRNTAKPFQVTFPHGKTQRDGRKESCKPILKVEYKTSRNSSEAFVIFSGG 321

  Fly   308 MPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLT 372
            :..:|..:...:|:|.|||.|||||::.:.||:.|||.|:..|.|||||:.|||:.|.|::||..
Zfish   322 LSYDKAGRRPTLTIMHGKAITVLEMDYPIVDFMVLCETPYLNEVQEPYAVVVLLEKDFVVVDLTQ 386

  Fly   373 PGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPA 437
            ..||.||:|||||:|||||||..|..|||.|::|..||:|  ...||:.:|.:|||:|||.|:..
Zfish   387 SNFPIFENPYPMDVHESPVTCTAYFADCPPDIIPVLYSIG--AKHKKTGYSHKEWPVSGGTWTVG 449

  Fly   438 SCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPE--SDNPLAVHLIF 500
            |.:|.||:||||.|||:||||:.|.|||:||||||:|:||||::.......:  .::|.||.:|.
Zfish   450 SQTYPEIIITGHADGSIKFWDATAITLQMLYKLKTSKVFEKPKTGDMGRSADLVEEDPYAVQMIS 514

  Fly   501 LCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTS-----PECDFLTGHQ 560
            .|.:||..||.|....|:|::|.|.::.:.:..||:.:..| .:|:...|     |.....:||.
Zfish   515 WCPQSRIFCVVGISAHVILYRFSKHDANTIITSLELRLQCE-MEDVISPSDTENTPCFSDPSGHS 578

  Fly   561 VQ------KAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVC-LTTGPNRRNVQVTSLCISSSYG 618
            .|      ::.:.||.:.:...|||::...|.|||:|::||. |.........|:|.|.::|:||
Zfish   579 PQPQPPSPRSNTPDSVRDSIPCLKVKDRMIRMPPGYQAELVVQLLWVDGEPPQQITCLDLNSAYG 643

  Fly   619 LMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKR------------IEN--KEE 669
            |:|.|...||.::|.:||..||.::..:|||:.||:.|..:||::            :.|  .:.
Zfish   644 LLALGNCNGLAVVDYLQKTILLCMSTLELYGSADPFQRLTRSPRKNRQSTSDFCMRGLSNFYSDS 708

  Fly   670 QSRSPSSDQLNDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAA 734
            :.|..:|.|.....|.:..||:.......|||           |.:|. :.|..|.|   |...:
Zfish   709 KKRIRTSYQSLTELSDNQVSLDLERSKSPTSA-----------PYTPS-LHNDPRGT---GEPVS 758

  Fly   735 TAAAAASAAVMM----AKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDME 795
            ...:...:.|..    .|.|.|..:.|.:|:                              ||::
Zfish   759 NLRSCHYSPVFYLLDNVKGPGRKLSLPTDLK------------------------------TDLD 793

  Fly   796 SLKNSSIFYTPKEVSPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRS 860
            .:....       .|| .:.|..:.....|.|.|...|            |...|.   |..||.
Zfish   794 HVNGHC-------TSP-TSQPCPSGRARVPGGPEGPRL------------ARRGPG---RPPFRK 835

  Fly   861 STTDSPDEGA--SSSIASGI--RRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQR 921
            :.:.:..|.:  .||:..|.  ||...:|..||.:                        .|.|:.
Zfish   836 AQSAACMEVSLPVSSLTEGYPSRRAMLQQLHGVSM------------------------YSHDEH 876

  Fly   922 LLSVPNIKYQPRDSRGGARNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRS 986
              .:.|..:..|:||                                           :.:||||
Zfish   877 --HITNYSWSERESR-------------------------------------------ENSFSRS 896

  Fly   987 RSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMP-ERDVRKSQP 1050
            ||||:||||..:.|:||.|.|.|||.:::|.|. .|.||:|||.|.:|.:.:::| :...|:..|
Zfish   897 RSSSVSSIDRETKEAVTTLQFAESYGRKSDSLP-TPCLWVGTSLGLVLIIPMSIPTDEQERQEDP 960

  Fly  1051 VLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKR-DNKDRTPTKSSSRTSPTFTPTT 1114
            |.:... |.|:.||||:....|||..|::|...:|.|||:.. |:.|| |.|   |....|:|
Zfish   961 VTVAPT-GTVLMLKGSVLRFGFLDCGGALINSPYEVWRDQHAPDDPDR-PRK---RKLVNFSP-- 1018

  Fly  1115 ANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGG 1179
                                                                             
Zfish  1019 ----------------------------------------------------------------- 1018

  Fly  1180 MSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETIT 1244
              |||..:.|             |....|:.||:|.|||.:.:|.|:....::|..|.::|:.::
Zfish  1019 --SSSQEACG-------------DGHLAVVCSERQAKVFYMPSQACLYVHNITESSFVLRADVVS 1068

  Fly  1245 MKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHED 1309
            :.:..|||.:.:|||:|:.|||||:.|||..:|||.::
Zfish  1069 VSNSVCLACFCANGHIMILSLPSLRPLLDVSYLPLTDM--------------------------- 1106

  Fly  1310 TTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFG 1374
              :|::.|||::.|..||:.||||||:.|.|.|.|..:.|.:|||:|..|.||....||.||.||
Zfish  1107 --RIARTFCFTNGGQALYLCSPTEIQRITYSQEMCVNLQETLGELFTPIETPEAQNRGFLKGFFG 1169

  Fly  1375 GGAKLLDREELFGEQS-GKPNRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLL 1437
            |.|:..||||||||.| ||.:||:|:||||. .:|.:...|.....:::||.....|||::|..|
Zfish  1170 GNAQTFDREELFGEASAGKASRSLAQHIPGQGGIEGMKAAAGGVVGDLARARIALDERGQRLGEL 1234

  Fly  1438 EERAERMANTAQDFSGTAHQLMLKYKDKKWYQ 1469
            |||...|..:|:.||..||:||||.|||||||
Zfish  1235 EERTALMMTSAETFSKHAHELMLKCKDKKWYQ 1266

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_727628.3	WD40	<41..257	CDD:441893	135/215 (63%)
		WD40 repeat	95..133	CDD:293791	25/37 (68%)
		WD40 repeat	138..184	CDD:293791	30/45 (67%)
		WD40 repeat	192..226	CDD:293791	18/33 (55%)
		LLGL	268..376	CDD:462446	51/108 (47%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	23/60 (38%)
stxbp5l	XP_005167782.1	WD40	<67..274	CDD:441893	127/208 (61%)
		WD40 repeat	67..103	CDD:293791	26/37 (70%)
		WD40 repeat	108..146	CDD:293791	25/37 (68%)
		WD40 repeat	151..199	CDD:293791	30/47 (64%)
		WD40 repeat	207..241	CDD:293791	19/33 (58%)
		WD40 repeat	246..284	CDD:293791	15/37 (41%)
		LLGL	281..390	CDD:462446	51/108 (47%)
		R-SNARE_STXBP5	1201..1260	CDD:277246	22/58 (38%)