DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tomosyn and stxbp5l

DIOPT Version :9

Sequence 1:	NP_001162735.1	Gene:	Tomosyn / 32217	FlyBaseID:	FBgn0030412	Length:	1470	Species:	Drosophila melanogaster
Sequence 2:	XP_005167782.1	Gene:	stxbp5l / 567605	ZFINID:	ZDB-GENE-041210-220	Length:	1267	Species:	Danio rerio

Alignment Length:	1527	Identity:	558/1527 - (36%)
Similarity:	779/1527 - (51%)	Gaps:	319/1527 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKKFTFKGVLDGFRQS--VQP---------------QATRQEQEIQEQLKADHFTLKKTFRHGFP 48
            |||  |:.||||...|  |.|               ..|.:|.||||.|.::||.:.||.|||||
Zfish     1 MKK--FRKVLDGLTTSSPVNPGGSPGCGSAAGTPSAAPTPRELEIQETLMSEHFQICKTVRHGFP 63

  Fly    49 YSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADD 113
            |.||:.|||||||:||||.:||.||||||||||.|::|  ||..|||..|||:|||||||..|||
Zfish    64 YQPTALAFDPVQKILAIGSRSGGIRILGRPGVDCHSQH--ESGAAVLQMQFLINEGALVTACADD 126

  Fly   114 TIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNK 178
            |:||||:||:.|.|:.||||.|||:|..|||..|||||||||:||.|:|:|::|.||||:|.|||
Zfish   127 TLHLWSLRQRLPAILHSLKFNRERITFCHLPFQSKWLYVGTERGNTHIVNIESFILSGYVIMWNK 191

  Fly   179 AIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGK 243
            |||:...:|||.|:.|.|:|.|..||||.||.|.:|:|||:||.|:.|....||:.|::||:||:
Zfish   192 AIELSTKTHPGPVVHLSDSPKDEGKLLIGFESGTIVMWDLRAKRADFRIYYDEAIHSVSWHHEGR 256

  Fly   244 YFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDGNAEKCKPIYKVDLKSSA-TGETFTIFSGG 307
            .|:.||:|||:..|..:..|||.....||.|..:||..|.||||.||:.|:|. :.|.|.|||||
Zfish   257 QFMCSHSDGSLSMWNMRNTAKPFQVTFPHGKTQRDGRKESCKPILKVEYKTSRNSSEAFVIFSGG 321

  Fly   308 MPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLT 372
            :..:|..:...:|:|.|||.|||||::.:.||:.|||.|:..|.|||||:.|||:.|.|::||..
Zfish   322 LSYDKAGRRPTLTIMHGKAITVLEMDYPIVDFMVLCETPYLNEVQEPYAVVVLLEKDFVVVDLTQ 386

  Fly   373 PGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPA 437
            ..||.||:|||||:|||||||..|..|||.|::|..||:|  ...||:.:|.:|||:|||.|:..
Zfish   387 SNFPIFENPYPMDVHESPVTCTAYFADCPPDIIPVLYSIG--AKHKKTGYSHKEWPVSGGTWTVG 449

  Fly   438 SCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPE--SDNPLAVHLIF 500
            |.:|.||:||||.|||:||||:.|.|||:||||||:|:||||::.......:  .::|.||.:|.
Zfish   450 SQTYPEIIITGHADGSIKFWDATAITLQMLYKLKTSKVFEKPKTGDMGRSADLVEEDPYAVQMIS 514

  Fly   501 LCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEIPILYENFDDIYGTS-----PECDFLTGHQ 560
            .|.:||..||.|....|:|::|.|.::.:.:..||:.:..| .:|:...|     |.....:||.
Zfish   515 WCPQSRIFCVVGISAHVILYRFSKHDANTIITSLELRLQCE-MEDVISPSDTENTPCFSDPSGHS 578

  Fly   561 VQ------KAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVC-LTTGPNRRNVQVTSLCISSSYG 618
            .|      ::.:.||.:.:...|||::...|.|||:|::||. |.........|:|.|.::|:||
Zfish   579 PQPQPPSPRSNTPDSVRDSIPCLKVKDRMIRMPPGYQAELVVQLLWVDGEPPQQITCLDLNSAYG 643

  Fly   619 LMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKR------------IEN--KEE 669
            |:|.|...||.::|.:||..||.::..:|||:.||:.|..:||::            :.|  .:.
Zfish   644 LLALGNCNGLAVVDYLQKTILLCMSTLELYGSADPFQRLTRSPRKNRQSTSDFCMRGLSNFYSDS 708

  Fly   670 QSRSPSSDQLNDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAA 734
            :.|..:|.|.....|.:..||:.......|||           |.:|. :.|..|.|   |...:
Zfish   709 KKRIRTSYQSLTELSDNQVSLDLERSKSPTSA-----------PYTPS-LHNDPRGT---GEPVS 758

  Fly   735 TAAAAASAAVMM----AKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDME 795
            ...:...:.|..    .|.|.|..:.|.:|:                              ||::
Zfish   759 NLRSCHYSPVFYLLDNVKGPGRKLSLPTDLK------------------------------TDLD 793

  Fly   796 SLKNSSIFYTPKEVSPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRS 860
            .:....       .|| .:.|..:.....|.|.|...|            |...|.   |..||.
Zfish   794 HVNGHC-------TSP-TSQPCPSGRARVPGGPEGPRL------------ARRGPG---RPPFRK 835

  Fly   861 STTDSPDEGA--SSSIASGI--RRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQR 921
            :.:.:..|.:  .||:..|.  ||...:|..||.:                        .|.|:.
Zfish   836 AQSAACMEVSLPVSSLTEGYPSRRAMLQQLHGVSM------------------------YSHDEH 876

  Fly   922 LLSVPNIKYQPRDSRGGARNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRS 986
              .:.|..:..|:||                                           :.:||||
Zfish   877 --HITNYSWSERESR-------------------------------------------ENSFSRS 896

  Fly   987 RSSSMSSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMP-ERDVRKSQP 1050
            ||||:||||..:.|:||.|.|.|||.:::|.|. .|.||:|||.|.:|.:.:::| :...|:..|
Zfish   897 RSSSVSSIDRETKEAVTTLQFAESYGRKSDSLP-TPCLWVGTSLGLVLIIPMSIPTDEQERQEDP 960

  Fly  1051 VLITINGGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKR-DNKDRTPTKSSSRTSPTFTPTT 1114
            |.:... |.|:.||||:....|||..|::|...:|.|||:.. |:.|| |.|   |....|:|
Zfish   961 VTVAPT-GTVLMLKGSVLRFGFLDCGGALINSPYEVWRDQHAPDDPDR-PRK---RKLVNFSP-- 1018

  Fly  1115 ANTLSNTSVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGG 1179

Zfish  1019 ----------------------------------------------------------------- 1018

  Fly  1180 MSSSSASSSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETIT 1244
              |||..:.|             |....|:.||:|.|||.:.:|.|:....::|..|.::|:.::
Zfish  1019 --SSSQEACG-------------DGHLAVVCSERQAKVFYMPSQACLYVHNITESSFVLRADVVS 1068

  Fly  1245 MKDGSCLATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHED 1309
            :.:..|||.:.:|||:|:.|||||:.|||..:|||.::
Zfish  1069 VSNSVCLACFCANGHIMILSLPSLRPLLDVSYLPLTDM--------------------------- 1106

  Fly  1310 TTQISKIFCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFG 1374
              :|::.|||::.|..||:.||||||:.|.|.|.|..:.|.:|||:|..|.||....||.||.||
Zfish  1107 --RIARTFCFTNGGQALYLCSPTEIQRITYSQEMCVNLQETLGELFTPIETPEAQNRGFLKGFFG 1169

  Fly  1375 GGAKLLDREELFGEQS-GKPNRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLL 1437
            |.|:..||||||||.| ||.:||:|:||||. .:|.:...|.....:::||.....|||::|..|
Zfish  1170 GNAQTFDREELFGEASAGKASRSLAQHIPGQGGIEGMKAAAGGVVGDLARARIALDERGQRLGEL 1234

  Fly  1438 EERAERMANTAQDFSGTAHQLMLKYKDKKWYQ 1469
            |||...|..:|:.||..||:||||.|||||||
Zfish  1235 EERTALMMTSAETFSKHAHELMLKCKDKKWYQ 1266

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_001162735.1	WD40	52..257	CDD:295369	126/204 (62%)
		WD40	<55..257	CDD:225201	125/201 (62%)
		WD40 repeat	95..133	CDD:293791	25/37 (68%)
		WD40 repeat	138..184	CDD:293791	30/45 (67%)
		WD40 repeat	192..226	CDD:293791	18/33 (55%)
		LLGL	269..375	CDD:285555	51/106 (48%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	23/60 (38%)
stxbp5l	XP_005167782.1	WD40	67..471	CDD:225201	230/407 (57%)
		WD40	67..>273	CDD:295369	127/207 (61%)
		WD40 repeat	67..103	CDD:293791	26/37 (70%)
		WD40 repeat	108..146	CDD:293791	25/37 (68%)
		WD40 repeat	151..199	CDD:293791	30/47 (64%)
		WD40 repeat	207..241	CDD:293791	19/33 (58%)
		WD40 repeat	246..284	CDD:293791	15/37 (41%)
		LLGL	282..389	CDD:285555	51/106 (48%)
		R-SNARE_STXBP5	1201..1260	CDD:277246	22/58 (38%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170594296
Domainoid	1	1.000	534	1.000	Domainoid score	I267
eggNOG	1	0.900	-	-		E1_KOG1983
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	937	1.000	Inparanoid score	I338
OMA	1	1.010	-	-		QHG49198
OrthoDB	1	1.010	-	-		D53111at33208
OrthoFinder	1	1.000	-	-		FOG0000702
OrthoInspector	1	1.000	-	-		otm26250
orthoMCL	1	0.900	-	-		OOG6_104979
Panther	1	1.100	-	-	O	PTHR10241
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2301
SonicParanoid	1	1.000	-	-		X453
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	15	14.800