DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tomosyn and stxbp5a

DIOPT Version :9

Sequence 1:	NP_001162735.1	Gene:	Tomosyn / 32217	FlyBaseID:	FBgn0030412	Length:	1470	Species:	Drosophila melanogaster
Sequence 2:	XP_009293002.1	Gene:	stxbp5a / 556789	ZFINID:	ZDB-GENE-041001-161	Length:	1142	Species:	Danio rerio

Alignment Length:	1513	Identity:	551/1513 - (36%)
Similarity:	759/1513 - (50%)	Gaps:	416/1513 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKKFTFKGVLDGF----RQSVQPQATRQEQE-IQEQLKADHFTLKKTFRHGFPYSPTSFAFDPVQ 60
            ||||..:.||||.    ..|..||...:|.| |.|.|:::||.|.||.||||||.|:|.||||||
Zfish     1 MKKFNIRKVLDGLTAVSSPSPSPQLGAKENELIPETLQSEHFQLCKTVRHGFPYQPSSMAFDPVQ 65

  Fly    61 KLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSIRQKTP 125
            |:||||.:||.:|:.|||||:.:.:|  ||..||:..|||:||||||:..|||:||||::|||.|
Zfish    66 KILAIGTQSGALRLFGRPGVECYCQH--ESGAAVIQLQFLINEGALVSALADDSIHLWNLRQKRP 128

  Fly   126 RIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRTSHPGA 190
            .::.||||.:||:|..|||..|||||:|:|:||||:|::::|.||||.|.||||||:...:|||.
Zfish   129 AVLHSLKFNKERITFCHLPFQSKWLYIGSERGNIHIVNVESFTLSGYTIMWNKAIELSSKAHPGP 193

  Fly   191 VIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHTDGSIC 255
            |:.:.|||:|..||:|.||.|::||||||:|.|:.|:...||:.|:|||:|||.|:.||:||::.
Zfish   194 VVHISDNPMDEGKLIIGFESGIVVLWDLKSKRADYRYTYDEAIHSVAWHHEGKQFICSHSDGTLT 258

  Fly   256 SWPTKPQAKPQSQVCPHAKINKDG-NAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGSKSNCI 319
            .|..|..|||...:.||.|..||| ..|.||||.||:.|::..|:.|.|||||:..:...:..|:
Zfish   259 IWNLKSPAKPIQTITPHGKQPKDGKKPEPCKPILKVEYKTTRAGDPFMIFSGGLSYDTVGRRPCL 323

  Fly   320 TVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFESPYPM 384
            |||.||:|.||||::.:.||:||||.|:|.:.|||||:.|||:.|||:|||...|:|.||:||||
Zfish   324 TVMHGKSTAVLEMDYPIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVVIDLAQNGYPIFENPYPM 388

  Fly   385 DLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEIVITGH 449
            .:||||||||.||.|||.||:||.||||  :..|:..:|::|||:|||.|...:.||.||:||||
Zfish   389 MIHESPVTCCEYLADCPVDLIPALYSVG--SRQKRQGYSKKEWPVSGGNWGLGTQSYPEIIITGH 451

  Fly   450 QDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPE--SDNPLAVHLIFLCSESRRLCVAG 512
            .|||:||||:.|..||:||||||||:|||.|........|  .::|.|:.|:..|.|||.|||||
Zfish   452 ADGSVKFWDASAIMLQVLYKLKTAKVFEKARCKEEKPSTEIVDEDPFAIQLMSWCPESRMLCVAG 516

  Fly   513 AMGQVMLFKFRKVESTSEVL-VLEIPILYENFDDIY---------------GTSPECDFLTGHQV 561
            ....|::::|.|.|.|:||: :||:.:.:| .:|:.               .|||... ..|.|.
Zfish   517 VSAHVIIYRFSKQEITTEVVQLLELRLQHE-LNDLESPEGGGDQTPAPPTPSTSPSPQ-TAGPQT 579

  Fly   562 QKAESSDSD----KTAECSLKVRNGAQRKPPGFQ----SQLVCLTTGPNRRNVQVTSLCISSSYG 618
            ..:.||:|.    ....| ||||:...::.||:|    .|||.::..|.:   |::||.|:|.||
Zfish   580 HTSSSSNSSDGMRDNVPC-LKVRSSPLKQSPGYQVELVVQLVWVSGEPPQ---QISSLAINSCYG 640

  Fly   619 LMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQLNDTT 683
            |:.:|...||.::|.:||..||::...:|||::|||.|.|:||::       ||.||
Zfish   641 LVVFGNSNGLAVVDYLQKTVLLNLGTVELYGSNDPYQRQPRSPRK-------SRQPS-------- 690

  Fly   684 SPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAASAAVMMAK 748
                        .|....::|.|| :..|..||..|:
Zfish   691 ------------GGLCDINEGSAT-SEDRCKSPTSVL---------------------------- 714

  Fly   749 SPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFYTPKEVSPEA 813
                                                            :.|...||
Zfish   715 ------------------------------------------------MSNHQHFY--------- 722

  Fly   814 ATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTDSPDEGASSSIASGI 878
                                       |.|.
Zfish   723 ---------------------------NGGG---------------------------------- 726

  Fly   879 RRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQRLLSV-PNIKYQPRDSRGGARNL 942
                    .|.::.|..:|     |..|||          |..|||| |.....|.|:|
Zfish   727 --------RGAKMSRKLSL-----PTELKP----------DLVLLSVCPPRGSIPLDNR------ 762

  Fly   943 KNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSSIDMSSSESVTCLAF 1007
                                                 |.:||||||||::|||..:.|::|...|
Zfish   763 -------------------------------------DNSFSRSRSSSVTSIDKETREAITSFYF 790

  Fly  1008 IESYAKRNDILTLVPTLWIGTSFGSILTLFITMPER-DVRKSQPVLITINGGPVVRLKGSITSMS 1071
            .|:|.::.| ..|.|:||:|||.|::|.:.:.||.. :.|..|||::: ..|.:.|||||:..|:
Zfish   791 CETYTRKAD-GGLTPSLWVGTSLGTVLAISLNMPPAGEQRLLQPVIVS-PCGTLFRLKGSVVRMA 853

  Fly  1072 FLDSYGSIIPYTFETWRD-------EKRDN-KDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGAS 1128
            ||||.|||:|..||.|.:       |:||. :.|.|...|..||
Zfish   854 FLDSTGSILPSPFEPWYEPNAPANGEERDKVRKRRPVSVSPSTS--------------------- 897

  Fly  1129 AGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGS 1193

Zfish   898 ----------------------------------------------------------------- 897

  Fly  1194 VSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNG 1258
                 :.|.:.||.|:.||||.||..:.:|.|:.:..::|..|.::|:.:.|..|.|:|.:.:||
Zfish   898 -----QELNENQYAVVCSEKQAKVVILPSQTCVFKHNITETSFVLRADVVQMNAGICVACFCANG 957

  Fly  1259 HLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKG 1323
            |:|..|||.|:.|||.::|||.::                             :|::.|||::.|
Zfish   958 HIMTLSLPGLRPLLDVNYLPLTDM-----------------------------RIARTFCFTNLG 993

  Fly  1324 HGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGE 1388
            ..||:.||||||:.|.|.|.|:.:.||:|||:|..|.||.|..|||||||||||:.|||.|||||
Zfish   994 QALYLTSPTEIQRITYSQEVCENLQEMLGELFTPVETPEAPNRGFFKGLFGGGAQSLDRMELFGE 1058

  Fly  1389 Q-SGKPNRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDF 1451
            . :||..||:|:||||| .:|.:...||....:::||.....|||:||:.:|||...|..:|..|
Zfish  1059 TGAGKAPRSLAQHIPGPGGIEGMKGAASGIVGDLARARIALDERGQKLSEVEERTAAMMASADSF 1123

  Fly  1452 SGTAHQLMLKYKDKKWYQ 1469
            |..||::|||.|||||||
Zfish  1124 SKHAHEMMLKCKDKKWYQ 1141

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_001162735.1	WD40	52..257	CDD:295369	116/204 (57%)
		WD40	<55..257	CDD:225201	115/201 (57%)
		WD40 repeat	95..133	CDD:293791	22/37 (59%)
		WD40 repeat	138..184	CDD:293791	28/45 (62%)
		WD40 repeat	192..226	CDD:293791	18/33 (55%)
		LLGL	269..375	CDD:285555	54/106 (51%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	24/60 (40%)
stxbp5a	XP_009293002.1	WD40	<42..270	CDD:225201	132/229 (58%)
		WD40	57..>275	CDD:295369	121/219 (55%)
		WD40 repeat	57..90	CDD:293791	20/32 (63%)
		WD40 repeat	98..136	CDD:293791	22/37 (59%)
		WD40 repeat	141..187	CDD:293791	28/45 (62%)
		WD40 repeat	195..231	CDD:293791	19/35 (54%)
		WD40 repeat	236..271	CDD:293791	17/34 (50%)
		LLGL	272..379	CDD:285555	54/106 (51%)
		R-SNARE_STXBP5	1075..1135	CDD:277246	23/59 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170594297
Domainoid	1	1.000	313	1.000	Domainoid score	I1272
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H16402
Inparanoid	1	1.050	937	1.000	Inparanoid score	I338
OMA	1	1.010	-	-		QHG49198
OrthoDB	1	1.010	-	-		D53111at33208
OrthoFinder	1	1.000	-	-		FOG0000702
OrthoInspector	1	1.000	-	-		otm26250
orthoMCL	1	0.900	-	-		OOG6_104979
Panther	1	1.100	-	-	LDO	PTHR10241
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2301
SonicParanoid	1	1.000	-	-		X453
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	15	14.940