DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tomosyn and Stxbp5l

DIOPT Version :9

Sequence 1:	NP_001162735.1	Gene:	Tomosyn / 32217	FlyBaseID:	FBgn0030412	Length:	1470	Species:	Drosophila melanogaster
Sequence 2:	NP_001258179.1	Gene:	Stxbp5l / 288080	RGDID:	1311561	Length:	1185	Species:	Rattus norvegicus

Alignment Length:	1520	Identity:	535/1520 - (35%)
Similarity:	754/1520 - (49%)	Gaps:	387/1520 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKKFTFKGVLDGFRQ-------------------SVQPQATR--QEQEIQEQLKADHFTLKKTFR 44
            ||||.|:.||||...                   ||.|..|.  ..:.|||.|.::.|.:.||.|
  Rat     1 MKKFNFRKVLDGLTASSPGSGSSSGSNSGGAGSGSVHPGGTAGVLREGIQESLISECFQICKTVR 65

  Fly    45 HGFPYSPTSFAFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTV 109
            |||||.||:.|||||||:||||.::|.||||||||||.:.:|  ||..|||..|||:||||||:.
  Rat    66 HGFPYQPTALAFDPVQKILAIGTRTGAIRILGRPGVDCYCQH--ESGAAVLQLQFLINEGALVSA 128

  Fly   110 TADDTIHLWSIRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYII 174
            ::|||:|||::|||.|.|:.||||.|||:|..|||..|||||||||:||.|:|:|::|.||||:|
  Rat   129 SSDDTLHLWNLRQKRPAILHSLKFNRERITYCHLPFQSKWLYVGTERGNTHIVNIESFILSGYVI 193

  Fly   175 NWNKAIEVVRTSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWH 239
            .||||||:...:|||.|:.|.|:|.|..||||.:|.|.:|.||||:|.||||....||:.|:.||
  Rat   194 MWNKAIELSTKTHPGPVVHLSDSPRDEGKLLIGYENGTVVFWDLKSKRAELRVYYDEAIHSIDWH 258

  Fly   240 YEGKYFVSSHTDGSICSWPTKPQAKPQSQVCPHAKINKDG-NAEKCKPIYKVDLKSSATGETFTI 303
            :|||.|:.||:|||:..|..|..::|.....||.|..::| .:|.||||.||:.|:....|.|.|
  Rat   259 HEGKQFMCSHSDGSLTLWNLKSPSRPFQTTVPHGKSQREGRKSESCKPILKVEYKTCRNSEPFII 323

  Fly   304 FSGGMPSEKGSKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLI 368
            ||||:..:|..:...:|:|.|||.|||||:|.:.:|:||||.|:|.|.|||||:||||:.||:::
  Rat   324 FSGGLSYDKACRRPSLTIMHGKAITVLEMDHPIVEFLTLCETPYPNEFQEPYAVAVLLEKDLIVV 388

  Fly   369 DLLTPGFPCFESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGE 433
            ||....||.||:|||||:|||||||..|..|||.||:...||:|  ...||..:|.:|||:|||.
  Rat   389 DLTQTNFPIFENPYPMDIHESPVTCTAYFADCPPDLILVLYSIG--VKHKKQGYSNKEWPVSGGA 451

  Fly   434 WSPASCSYSEIVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPE--SDNPLAV 496
            |:..:.:|.||:||||.||::||||:.|.|||:||||||:|:|||.:........|  .::|.||
  Rat   452 WNLGAQTYPEIIITGHADGTIKFWDASAMTLQMLYKLKTSKVFEKQKVGDGKQTCEIVEEDPFAV 516

  Fly   497 HLIFLCSESRRLCVAGAMGQVMLFKFRKVESTSEVLVLEI-------------PILYENFDDIYG 548
            .:|:.|.|||..||:|....|:::||.:.|.|:|::.||:             |.....|.|:..
  Rat   517 QMIYWCPESRIFCVSGVSAYVIIYKFSRHEITAEIVSLEVRLQCDVEDIITPEPETSPPFPDLSS 581

  Fly   549 TSPECDFLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQS----QLVCLTTGPNRRNVQVT 609
            ..|....|:| ......|....|.:...|.|:....|.|||:|:    |||.:...|.:   |:|
  Rat   582 QLPSSRSLSG-STNTVSSEGVTKDSIPCLSVKTRPVRMPPGYQADLVIQLVWVDGEPPQ---QIT 642

  Fly   610 SLCISSSYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSP 674
            ||.:||:||::|:|...|||::|.|||..|||:...|||.:.|.|.|.|:||::
  Rat   643 SLAVSSAYGIVAFGNCTGLVVVDFIQKTVLLSMGTIDLYRSSDLYQRQPRSPRK----------- 696

  Fly   675 SSDQLNDTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAA 739
                          :.:|:.:
  Rat   697 --------------NRQFIAD-------------------------------------------- 703

  Fly   740 ASAAVMMAKSPQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFY 804
                                                               |..|..|.|   ||
  Rat   704 ---------------------------------------------------NFCMRGLSN---FY 714

  Fly   805 TPKEVSPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTD----- 864
                  |:......|..::..|      |:|             ||.|....:.:|.|:|
  Rat   715 ------PDLTKRIRTSYQSLTE------LND-------------SPVPLELERCKSPTSDHVNGH 754

  Fly   865 --SPDEGASSSIASGIRRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKF-KEKSRDQRLLSVP 926
              ||   .|.|.:||.|.      :..:|.:.|.         ..|.|..| |.:|.....:|:|
  Rat   755 CTSP---TSQSCSSGKRL------SSADVSKVNR---------WGPGRPPFRKAQSAACMEISLP 801

  Fly   927 NIKYQPRDSRGGARNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSM 991
            ....:.|::                                              :::||||||:
  Rat   802 VTTEETREN----------------------------------------------SYNRSRSSSI 820

  Fly   992 SSIDMSSSESVTCLAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPERDVRKSQPVLITIN 1056
            ||||..|.|::|.|.|:||:|::|| .|:.|.|::|||.|.::.:.:.:|..|.::....::.:.
  Rat   821 SSIDKDSKEAITALYFMESFARKND-STVSPCLFVGTSLGMVILISLNLPSSDEQRFTEPVVVLP 884

  Fly  1057 GGPVVRLKGSITSMSFLDSYGSIIPYTFETWRDEKRDNKDRTPTKSSSRTSPTFTPTTANTLSNT 1121
            .|..:.|||::.:.|.:|..|:::...:|.|||                  |..|.....|....
  Rat   885 SGTFLSLKGAVLTFSCMDRTGNLMQPPYEVWRD------------------PNNTDENEKTWKRK 931

  Fly  1122 SVAGGASAGGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSAS 1186
            .|                                                        |:.||:|
  Rat   932 LV--------------------------------------------------------MNYSSSS 940

  Fly  1187 SSGISGSVSTGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCL 1251
                        :.:.|.||.:|.||||.|||.:.:|.|:....::|..|.::|:.:.|.:.:||
  Rat   941 ------------QEMGDHQYTIICSEKQAKVFSLPSQTCLYVHNITETSFILQADVVVMCNSACL 993

  Fly  1252 ATYLSNGHLMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKI 1316
            |.:.:|||:|:.|||||:.:||.::|||.::                             :|::.
  Rat   994 ACFCANGHIMIMSLPSLRPMLDVNYLPLTDM-----------------------------RIART 1029

  Fly  1317 FCFSHKGHGLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLD 1381
            |||:::|..||:.||||||:.|.|.|.|..|.:|:|:|:|..|.||....||.||||||..:..|
  Rat  1030 FCFTNEGQALYLVSPTEIQRLTYSQEMCDNIQDMLGDLFTPIETPEAQNRGFLKGLFGGSGQTFD 1094

  Fly  1382 REELFGEQS-GKPNRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERM 1444
            |||||||.| ||.:||:|:||||| ::|.:...|.....|::||.....|||::|..|||:...|
  Rat  1095 REELFGEASAGKASRSLAQHIPGPGSIEGMKGAAGGVMGELTRARIALDERGQRLGELEEKTAGM 1159

  Fly  1445 ANTAQDFSGTAHQLMLKYKDKKWYQ 1469
            ..:|:.||..||:||||||||||||
  Rat  1160 MTSAEAFSKHAHELMLKYKDKKWYQ 1184

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_001162735.1	WD40	52..257	CDD:295369	124/204 (61%)
		WD40	<55..257	CDD:225201	123/201 (61%)
		WD40 repeat	95..133	CDD:293791	23/37 (62%)
		WD40 repeat	138..184	CDD:293791	30/45 (67%)
		WD40 repeat	192..226	CDD:293791	18/33 (55%)
		LLGL	269..375	CDD:285555	52/106 (49%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	24/60 (40%)
Stxbp5l	NP_001258179.1	WD40	73..>280	CDD:295369	125/208 (60%)
		WD40 repeat	73..106	CDD:293791	23/32 (72%)
		WD40	108..503	CDD:225201	217/396 (55%)
		WD40 repeat	114..152	CDD:293791	23/37 (62%)
		WD40 repeat	210..247	CDD:293791	21/36 (58%)
		WD40 repeat	252..288	CDD:293791	15/35 (43%)
		LLGL	288..395	CDD:285555	52/106 (49%)
		R-SNARE_STXBP5	1118..1178	CDD:277246	23/59 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166352362
Domainoid	1	1.000	344	1.000	Domainoid score	I1024
eggNOG	1	0.900	-	-		E1_KOG1983
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	953	1.000	Inparanoid score	I317
OMA	1	1.010	-	-		QHG49198
OrthoDB	1	1.010	-	-		D53111at33208
OrthoFinder	1	1.000	-	-		FOG0000702
OrthoInspector	1	1.000	-	-		otm44543
orthoMCL	1	0.900	-	-		OOG6_104979
Panther	1	1.100	-	-	O	PTHR10241
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X453
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.770