DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Tomosyn and stxbp5

DIOPT Version :9

Sequence 1:	NP_001162735.1	Gene:	Tomosyn / 32217	FlyBaseID:	FBgn0030412	Length:	1470	Species:	Drosophila melanogaster
Sequence 2:	XP_012819238.1	Gene:	stxbp5 / 100488413	XenbaseID:	XB-GENE-1012360	Length:	1151	Species:	Xenopus tropicalis

Alignment Length:	1512	Identity:	546/1512 - (36%)
Similarity:	775/1512 - (51%)	Gaps:	405/1512 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKKFT-FKGVLDGF----------RQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSF 54
            ||||. .:.||||.          ..|.||  ..:|.||.|.|:::||.|.||.||||||.|::.
Frog     1 MKKFNPIRKVLDGLTAVSSSSSSSSSSCQP--GNKELEITETLQSEHFQLCKTVRHGFPYQPSAL 63

  Fly    55 AFDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWS 119
            |||||||:||||.::|.:|:.|||||:.:.:|  :|..||:..|||:||||||:...|||:|||:
Frog    64 AFDPVQKILAIGTQTGALRLFGRPGVECYCQH--DSGAAVIQLQFLINEGALVSALTDDTLHLWN 126

  Fly   120 IRQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVR 184
            :|||.|.::.||||.|||:|..|||..|||||||||:||||:|::::|.||||:|.||||||:..
Frog   127 LRQKRPAVLHSLKFSRERITFCHLPFQSKWLYVGTERGNIHIVNVESFTLSGYVIMWNKAIELSS 191

  Fly   185 TSHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSH 249
            .||||.|:.:.|||:|..||||.||.|::||||||:|.|:.|:...||:.|:|||:|||.|:.||
Frog   192 KSHPGPVVHISDNPMDEGKLLIGFESGIVVLWDLKSKKADYRYTYDEAIHSVAWHHEGKQFICSH 256

  Fly   250 TDGSICSWPTKPQAKPQSQVCPHAKINKDG-NAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKG 313
            :||::..|..:..||....:.||.|..||| ..|.||||.||:.|::..|:.|.|.|||:..:..
Frog   257 SDGTLTIWNVRSPAKCIQTIVPHGKQIKDGKKPEPCKPILKVEYKTTRYGDPFIILSGGLSYDTV 321

  Fly   314 SKSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCF 378
            .:..|:|||.||:|.||||::.:.||:||||.|:|.:.|||||:.|||:.|||:|||...|:|.|
Frog   322 GRRPCLTVMHGKSTAVLEMDYPIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVVIDLAQNGYPIF 386

  Fly   379 ESPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSE 443
            |:|||:.:||||||||.|..|||.||:||.||||  ...|:..:|::|||||||.|...:.||.|
Frog   387 ENPYPLSIHESPVTCCEYFADCPVDLIPALYSVG--ARQKRQGYSKKEWPISGGNWGVGTQSYPE 449

  Fly   444 IVITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESD----NPLAVHLIFLCSE 504
            |:||||.|||:||||:.|.|||:||||||||:|||.|:  ....|.:|    :|.|:.:|..|.|
Frog   450 IIITGHADGSIKFWDASAITLQVLYKLKTAKVFEKSRN--KDDKPSTDIVDEDPYAIQMISWCPE 512

  Fly   505 SRRLCVAGAMGQVMLFKFRKVESTSEVL-VLEIPILYENFDDIYGTSPECD-------------- 554
            ||.|||||....|::::|.|.|.|:||: :||:.:||| .:|:  .||:.:
Frog   513 SRVLCVAGVSAHVIIYRFSKQEVTTEVVSMLEVRLLYE-INDL--ESPDIEQVPTPSGGPNSQPI 574

  Fly   555 FLTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQ----SQLVCLTTGPNRRNVQVTSLCISS 615
            ....|....:.|||..:.....|:|::.|.::..|:|    .|||.::..|.:   |:|||.::|
Frog   575 VFQSHPSTSSSSSDGIRDNVPCLRVKSTALKQSAGYQVELVIQLVWVSGEPPQ---QITSLAVNS 636

  Fly   616 SYGLMAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQLN 680
            :|||:.:|...|:.|:|.|||..||:::..:|||::|||.|.|:||::       :|.||...|:
Frog   637 AYGLVVFGNCNGIAIVDYIQKAVLLNLSTVELYGSNDPYQRQPRSPRK-------TRQPSGAGLS 694

  Fly   681 DTTSPDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAASAAVM 745
            |....                    ..|:..|..||                  |:...::::
Frog   695 DINES--------------------TVASEDRCKSP------------------TSGCTSNSS-- 719

  Fly   746 MAKSPQRDDAAPKELQDRRKSMSWKTFNLK-RQLSKVNMKIGGLNVNTDMESLKNSSIFYTPKEV 809
                   ||       ::.||...:...:| |:.||.        :.||:  .|.|.....|.|:
Frog   720 -------DD-------EQTKSKFIEKVKIKGRRFSKA--------IVTDL--AKMSRKLSLPTEL 760

  Fly   810 SPEAATPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTDSPDEGASSSI 874
            .||.
Frog   761 KPEL------------------------------------------------------------- 764

  Fly   875 ASGIRRVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQRLLSVPNIKYQPRDSRGGA 939
                                  :|..|.
Frog   765 ----------------------VPFLDV------------------------------------- 770

  Fly   940 RNLKNDVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSSIDMSSSESVTC 1004
                                                   .|.:||||||||::|||..|.|:::.
Frog   771 ---------------------------------------RDNSFSRSRSSSVTSIDKESREAISS 796

  Fly  1005 LAFIESYAKRNDILTLVPTLWIGTSFGSILTLFITMPE-RDVRKSQPVLITINGGPVVRLKGSIT 1068
            |.|.|::.::.|:.| .|.||:||:.|::|.:.:.:|. .:.|..|||::: ..|.::||||:|.
Frog   797 LHFCETFTRKPDVST-SPCLWVGTTLGTVLVIVLNLPPGGEQRLLQPVIVS-PSGTILRLKGAIL 859

  Fly  1069 SMSFLDSYGSIIPYTFETWRD----EKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASA 1129
            .|||||:.|::||...|:||:    |.:|:|||...:.....||
Frog   860 RMSFLDTLGALIPPASESWREPNVPEDKDDKDRLKKRRPVSVSP--------------------- 903

  Fly  1130 GGGAGGGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSV 1194
                                                                    |||
Frog   904 --------------------------------------------------------SSS------ 906

  Fly  1195 STGLETLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGH 1259
                :.:::.||.||.||||.||..:.:|.||.::.::|..|.::.:.::|.:.:|||.:.:|||
Frog   907 ----QEISENQYAVICSEKQAKVISLPSQNCIYKLNITETSFVLRGDIVSMSNSTCLACFCANGH 967

  Fly  1260 LMVHSLPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGH 1324
            :|..|||||:.|||.::|||..:                             :|::.|||::.|.
Frog   968 IMTFSLPSLRPLLDVNYLPLTNM-----------------------------RIARTFCFTNNGQ 1003

  Fly  1325 GLYMASPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGE- 1388
            .||:.||||||:.|.|.|.|:.:.||:|||:|..|.||.|..|||||||||||:.||||:||||
Frog  1004 ALYLVSPTEIQRITYSQETCENLQEMLGELFTPVETPEAPNRGFFKGLFGGGAQSLDREDLFGET 1068

  Fly  1389 QSGKPNRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFS 1452
            .||||:||:|:||||| .:|.:...||....|::||.....|||:|:..|||:...|..:|..||
Frog  1069 ASGKPSRSLAQHIPGPGGIEGVKGAASGVVGELARARWALDERGQKIGELEEKTTAMLASADSFS 1133

  Fly  1453 GTAHQLMLKYKDKKWYQ 1469
            ..||::|||||||||||
Frog  1134 KHAHEMMLKYKDKKWYQ 1150

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Tomosyn	NP_001162735.1	WD40	52..257	CDD:295369	117/204 (57%)
		WD40	<55..257	CDD:225201	117/201 (58%)
		WD40 repeat	95..133	CDD:293791	21/37 (57%)
		WD40 repeat	138..184	CDD:293791	30/45 (67%)
		WD40 repeat	192..226	CDD:293791	19/33 (58%)
		LLGL	269..375	CDD:285555	53/106 (50%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	24/60 (40%)
stxbp5	XP_012819238.1	WD40	61..>283	CDD:392136	123/223 (55%)
		WD40 repeat	61..94	CDD:293791	18/32 (56%)
		WD40 repeat	102..140	CDD:293791	21/37 (57%)
		WD40 repeat	145..191	CDD:293791	30/45 (67%)
		WD40 repeat	199..235	CDD:293791	20/35 (57%)
		WD40 repeat	240..275	CDD:293791	15/34 (44%)
		LLGL	275..383	CDD:369834	53/107 (50%)
		R-SNARE_STXBP5	1084..1144	CDD:277246	23/59 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	446	1.000	Domainoid score	I522
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H16402
Inparanoid	1	1.050	1003	1.000	Inparanoid score	I298
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D53111at33208
OrthoFinder	1	1.000	-	-		FOG0000702
OrthoInspector	1	1.000	-	-		otm47581
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X453
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	7	7.060